Valorization of agro-industrial wastes towards the production of rhamnolipids

Abstract

In this work, oil mill wastewater (OMW), a residue generated during olive oil extraction, was evaluated as an inducer of rhamnolipid production. Using a medium containing as sole ingredients corn steep liquor (10%, v/v), sugarcane molasses (10%, w/v) and OMW (25%, v/v), Pseudomonas aeruginosa #112 produced 4.5 and 5.1g of rhamnolipid per liter in flasks and reactor, respectively, with critical micelle concentrations as low as 13mg/l. Furthermore, in the medium supplemented with OMW, a higher proportion of more hydrophobic rhamnolipid congeners was observed comparing with the same medium without OMW. OMW is a hazardous waste which disposal represents a serious environmental problem; therefore, its valorization as a substrate for the production of added-value compounds such as rhamnolipids is of great interest. This is the first report of rhamnolipid production using a mixture of these three agro-industrial by-products, which can be useful for the sustainable production of rhamnolipids.

Utilization of mango kernel oil for the rhamnolipid production by Pseudomonas aeruginosa DR1 towards its application as biocontrol agent

“…In this study, rhamnolipids containing single fatty acid congeners including mono-rhamnolipid (Rha-C 10 , Rha-C 8 ) and di-rhamnolipid (Rha-Rha-C 10 , Rha-Rha-C 12:1 , Rha-Rha-C 8 ) were also detected. The occurrence of similar single fatty acid rhamnolipid congeners were also reported in the previous studies (Gudiña et al, 2016;Nie et al, 2010). The unsaturated rhamnolipids constituted 15.52% (MKO) and 19.39% (MKO with glucose) from the total rhamnolipid mixture (Table 3).…”

“…According to the literature various types of agriculture and industrial byproducts were used as carbon source for the rhamnolipid production (Da Silva et al, 2009;Gudiña et al, 2016). In the present study, mango kernel oil (MKO) and synthetic fatty acids (palmitic, stearic and oleic acid) were tested for their use as substrates for rhamnolipid production.…”

“…The surfactant properties of rhamnolipids are found to be influenced by media composition, employed microbial strain used and fermentation conditions. In addition to reducing the surface/interfacial tension and emulsification activity, rhamnolipids are found to show other properties like wetting and foaming based on their congeners (Gudiña et al, 2016) thereby increasing applicability in various fields.…”

Biosurfactant trehalose lipid‐enhanced ultrasound‐assisted micellar extraction and determination of the main antioxidant compounds from functional plant tea

“…Surfactants, due to the presence of hydrophilic and hydrophobic regions, can accumulate at the interfaces between water and hydrocarbons, and this accumulation can reduce surface are three kinds of widely used glycolipids. It is reported that these biosurfactants have been employed broadly for various applications covering industrial and environmental fields [7][8][9]. However, few extraction methods employing biosurfactants have been reported, limited to just the biodispersive liquid microextraction based on nano rhamnolipid aggregates [10] and the reverse micelle extraction of antibiotics using sophorolipids [11].…”

Advances in applications of rhamnolipids biosurfactant in environmental remediation: A review

“…Using renewable resources has advantages of reducing the cost of production and alleviating the environmental problems related to the residue disposal. The residues, for example, waste frying oils (George & Jayachandran, 2013;Luo et al, 2013), waste cooking oil (Lan et al, 2015), sugarcane molasses (Gudiña et al, 2016) and oilcontaining wastewater (Yin et al, 2009) have been proposed as alternative substrates to produce rhamnolipids.…”