Valorization of Brewer’s spent grain to prebiotic oligosaccharide: Production, xylanase catalyzed hydrolysis,  in-vitro  evaluation with probiotic strains and in a batch human fecal fermentation model

Sajib, Mursalin; Falck, P; Sardari, Roya R.R.; Mathew, Sindhu; Grey, Carl; Karlsson, Eva Nordberg; Adlercreutz, Patrick

doi:10.1016/j.jbiotec.2018.01.005

Cited by 58 publications

(38 citation statements)

References 35 publications

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“…The results suggest that the utilization rate is more dependent on the type of prebiotic added, rather than the differences between the gut microbiota of the donors, similarly to the previous observation for SCFAs production. The combination of prebiotics with different fermentation rates can promote fermentation in large parts of the colon, thus potentiating the prebiotic effect (Sajib et al, 2018). Therefore, the supplementation with one or more prebiotic can potentially add value to a product.…”

Section: Resultsmentioning

confidence: 99%

“…Xylooligosaccharides (XOS) have been identified as potentially valuable food and feed prebiotic ingredients (Sajib et al, 2018) and are the only nutraceuticals that can be produced from cheap and abundant lignocellulosic biomass (Samanta et al, 2015). The gut microbiota uses prebiotics to multiply and consequently produce short-chain fatty acids (SCFAs), gases (mainly, hydrogen and carbon dioxide), lactate, and other products (Topping & Clifton, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, XOS presented high stability after a static in vitro digestion. However, this method is not elucidative of their prebiotic effect, which depends both on their degree of polymerization (DP) and degree of substitution (Sajib et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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In vitro assessment of prebiotic properties of xylooligosaccharides produced by Bacillus subtilis 3610

Amorim

Silvério

Cardoso

et al. 2020

Carbohydrate Polymers

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Xylooligosaccharides (XOS) are emergent prebiotics exhibiting high potential as food ingredients. In this work, in vitro studies were performed using human fecal inocula from two healthy donors (D 1 and D2) to evaluate the prebiotic effect of commercial lactulose and XOS produced in a single-step by recombinant Bacillus subtilis 3610. The fermentation of lactulose led to the highest production of lactate (D1: 33.7 ± 0.5 mM; D2:19.7 ± 0.3 mM) and acetate (D1: 77.5 ± 0.6 mM; D2: 81.0 ± 0.7 mM), while XOS led to the highest production of butyrate (D1: 9.0 ± 0.6 mM; D2: 10.5 ± 0.8 mM) and CO 2 (D1: 8.92 ± 0.02 mM; D2: 11.4 ± 0.3 mM). Microbiota analysis showed a significant decrease in the relative abundance of Proteobacteria for both substrates and an increase in Bifidobacterium and Lactobacillus for lactulose, and Bacteroides for XOS.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro assessment of prebiotic properties of xylooligosaccharides produced by Bacillus subtilis 3610

Amorim

Silvério

Cardoso

et al. 2020

Carbohydrate Polymers

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show abstract

“…brevis and B . adolescentis have shown to be able to utilize arabinoxylans and AXOS from brewer’s spent grain; however, the results showed a similar or slightly less efficiency compared to commercial XOS, FOS, and galactooligosaccharides (Sajib et al, ). Furthermore, AXOS from brewer’s spent grain showed a higher number of bifidobacteria compared to commercial FOS; however, lactobacilli growth was lower than FOS.…”

Section: Oligosaccharides As Prebiotic Compoundsmentioning

confidence: 98%

“…Some researchers have assessed the content of this OS in agro‐industrial by‐products; for example, barley flour contains 5.02% arabinoxylan and brewer's spent grain 14.75% arabinoxylan (Sajib et al, ). Also, Gomez, Miguez, Veiga, Parajo, and Alonso () reported that brewer's spent grain is composed of glucan (24.91%), xylan (13.94%), arabinan (6.68%), and acid insoluble residue (22.82%).…”

Section: Oligosaccharides As Prebiotic Compoundsmentioning

confidence: 99%

Prebiotic compounds from agro‐industrial by‐products

2018

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Prebiotics are nondigestible food components that have an impact on gut microbiota composition and activity, which in turn results in the improvement of health conditions. Nowadays, the production of prebiotics from agro‐industrial by‐products is under investigation. In this regard, polysaccharides are usually found in these sources and their potential use as prebiotics has been studied recently since these compounds act as substrates for the human gut microbiota, and they have the potential to modulate its composition through many mechanisms. Additionally, the use of agricultural by‐products is advantageous because it is a cheap and abundantly available material. This review focuses on the recent scientific literature regarding the prebiotic properties of polysaccharides from agro‐industrial by‐products. Practical applications Currently, the maintenance of gut homeostasis is a target for the improvement of human health. This review can broaden the perspective on the utilization of agro‐industrial by‐products that can compete in the market with the commercial ones or act as a source for new food ingredients.

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Novel Function of CtXyn5A from Acetivibrio thermocellus: Dual Arabinoxylanase and Feruloyl Esterase Activity in the Same Active Site**

et al. 2022

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Enzymes' uncharacterised side activities can have significant effects on reaction products and yields. Hence, their identification and characterisation are crucial for the development of successful reaction systems. Here, we report the presence of feruloyl esterase activity in CtXyn5A from Acetivibrio thermocellus, besides its well-known arabinoxylanase activity, for the first time. Activity analysis of enzyme variants mutated in the catalytic nucleophile, Glu279, confirmed removal of all activity for E279A and E279L, and increased esterase activity while removing xylanase activity for E279S, thus allowing the proposal that both reaction types are catalysed in the same active site in two subsequential steps. The ferulic acid substituent is cleaved off first, followed by hydrolysis of the xylan backbone. The esterase activity on complex carbohydrates was found to be higher than that of a designated ferulic acid esterase (E-FAERU). Therefore, we conclude that the enzyme exhibits a dual function rather than an esterase side activity.

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Valorization of Brewer’s spent grain to prebiotic oligosaccharide: Production, xylanase catalyzed hydrolysis, in-vitro evaluation with probiotic strains and in a batch human fecal fermentation model

Cited by 58 publications

References 35 publications

In vitro assessment of prebiotic properties of xylooligosaccharides produced by Bacillus subtilis 3610

In vitro assessment of prebiotic properties of xylooligosaccharides produced by Bacillus subtilis 3610

Prebiotic compounds from agro‐industrial by‐products

Novel Function of CtXyn5A from Acetivibrio thermocellus: Dual Arabinoxylanase and Feruloyl Esterase Activity in the Same Active Site**

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