Valorization of low-cost agro-wastes residues for the maximum production of protease and lipase haloextremozymes by Haloferax lucentensis GUBF-2 MG076078

Gaonkar, Sanket K.; Furtado, Irene

doi:10.1016/j.procbio.2020.10.019

Cited by 25 publications

(13 citation statements)

References 68 publications

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“…The procurement, processing, and characterization of FW and COC were done in accordance with Gaonkar and Furtado [7]. In outline, FW and COC were collected from local Goan chicken abattoirs and oil milling industries, respectively.…”

Section: Culturing Of Haloferax In Low Nacl Medium Containing Feather Waste (Fw) For Production Of Haloextremozyme Protease and Lipasementioning

confidence: 99%

“…The percentage raw feather/COC degraded by GUBF-2 in a reactor after fermentation was expressed as % degradation after fermentation; calculated according to the relation given by Gaonkar and Furtado [7] as shown in Eq. 1.…”

Section: Characterization Of Feather and Coc Hydrolysatementioning

confidence: 99%

“…While lipase from CFFH produced constant zone of 8.5 mm ± 1.50 compared to CFS when GUBF-2 grew in medium-5 (12.0 ± 1.32 mm) (Online resource 1b). It is reported that Haloferax lucentensis GUBF-2 MG076078 produced protease and lipase haloextremozymes in the presence of 5% to 35% NaCl with optimum production at 30% NaCl concentration [7].…”

Section: Haloextremozyme Protease and Lipase Secretion By Haloferax Lucentensis Gubf-2 Mg076878 During Growth In Low Salt On Feather-wastmentioning

confidence: 99%

“…Feathers represent 5-7% of the total weight of mature chicken and constitute 80-90% keratin and 12-15% nitrogen [7][8][9]. Feather hydrolysate produced by bacteria is used for the synthesis of bioplastic [10], nanoparticles [11] and in slow-release nitrogen fertilizers [3,[12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, Gaonkar and Furtado [7] reported the biodegradation of nine different agro-food wastes by Haloferax lucentensis GUBF-2 MG076878 (Haloferax sp. strain GUBF-2 ATCC BAA 645) for the simultaneous production of protease and lipase haloextremozymes in a shake flask under optimum conditions of pH 6, 42 °C, and 30% NaCl.…”

Section: Introductionmentioning

confidence: 99%

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Biorefinery-Fermentation of Agro-Wastes by Haloferax lucentensis GUBF-2 MG076878 to Haloextremozymes for use as Biofertilizer and Biosynthesizer of AgNPs

Gaonkar

Furtado

2021

Waste Biomass Valor

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Purpose The green biorefinery approach is an alternative to the chemical method of production of enzyme-rich hydrolysate from waste biomass. Specifically, feather and coconut oil cake (COC) hydrolysate manifests the generation of bioproducts like dietary fibers, biofertilizers, bioplastics, esters, nanoparticles, etc. Methods We scaled up the production of haloextremozyme-rich hydrolysates using Haloferax lucentensis GUBF-2 MG076878 in an economical bioreactor for valuation as biofertilizer for rice growth and biosynthesis of silver nanoparticles (AgNPs), showing antimicrobial activity. ResultsThe strain solubilizes 10% feather and COC with 78.75 ± 0.717% and 88.35 ± 0.654% degradation after 20 days, respectively. Moreover, tangential filtration aided co-concentration of both protease and lipase from feather/COC hydrolysate to >70% yield. SEM of feather/COC hydrolysate depicted particle size ranging 100-10000 nm. FTIR evidenced the functional groups of >C=O, -CH, -NH, -CH3, OH, and COO−. Priming seeds of rice with feather hydrolysate resulted in 100% germination energy and higher vigor index ( 1214), alongside increased shoot length (43.2 ± 0.58) under saline conditions. Biosynthesized AgNPs showed absorption maxima at 440 nm and vibrations of -CH, -OH, -NH, >C=C, and >C=O functionality in FTIR. AFM depicted the semi-oval morphology of AgNPs with a maximum height of 22 nm. Also, the presence of silver was confirmed by SEM-EDAX. AgNPs exhibited antimicrobial activity against human pathogens as; C. albicans>S. aureus>S. pyogenes>E. coli ATCC 8439>P.vulgaris, and S.typhi. Conclusion Conclusively, the production of haloextremozyme-rich feather and COC hydrolysate via the biorefinery approach employing Haloferax lucentensis GUBF-2; is lucrative, considering waste valorization and biosynthesis of bioproducts in biotechnological applications.

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Section: Culturing Of Haloferax In Low Nacl Medium Containing Feather Waste (Fw) For Production Of Haloextremozyme Protease and Lipasementioning

confidence: 99%

Section: Characterization Of Feather and Coc Hydrolysatementioning

confidence: 99%

Section: Haloextremozyme Protease and Lipase Secretion By Haloferax Lucentensis Gubf-2 Mg076878 During Growth In Low Salt On Feather-wastmentioning

confidence: 99%