Valorization of Potato Peels Starch for Efficient β‐Cyclodextrin Production and Purification through an Eco‐Friendly Process

Abstract

Potato peels (PP) are agro‐industrial co‐products generated in huge quantities by the potato processing industry. Obtaining value‐added products from PP is a current research challenge. As cyclic oligosaccharides is produced from starch by the action of cyclodextrin glucanotransferase (CGTase), β‐cyclodextrins (β‐CDs) are increasingly used in various industrial applications. In an attempt to develop a low‐cost process for β‐CDs production, starch is firstly extracted from PP. The productions of the US132 CGTase and β‐CDs are then optimized via experimental designs. The US132 CGTase production from the recombinant T7 express Escherichia coli strain is enhanced 20‐fold to reach 59.95 U mL−1. Subsequently, the β‐CDs production by the US132 CGTase is optimized to reach a high level (67.26 g L−1). The β‐CDs are then purified to homogeneity through a simple eco‐friendly crystallization method that provided a purification yield of 20%. This study reveals that β‐CDs production from valorized starch is a promising strategy used to valorize PP and minimize β‐CDs production cost.

Biokatalytische Entstehung von Sesquiterpenoiden

“…Produktionskosten konnte ein Prozess etabliert werden, in dem Kartoffelstärke mit einer heterolog produzierten Cyclodextrin-Glucanotransferase umgesetzt wird (Amara et al, 2022). Neben dem Einsatz als Agens zur Herstellung von Filmen für technische Anwendungen (Javanbakht & Namazi, 2017) wird Stärke vor allem für die Herstellung von Aerogelen und Schäumen genutzt und dient damit überwiegend der Verpackungsindustrie (Silva et al, 2020).…”

Systematic Review on Characterization of Tannase from Agricultural By-Products

“…Alternatively, polysaccharides may be converted into valuable biomolecules. It has been found that the enzymatic degradation of water-soluble polysaccharides in potato peel generated functional oligosaccharides (Ben Amara et al, 2022).…”

Molecular Mind Games: The Medicinal Action of Cyclodextrins in Neurodegenerative Diseases

“…Cyclodextrins (CDs) are cyclic oligosaccharides that can occur spontaneously in the natural environment by bacterial fermentation of starch [ 1 , 2 ] or be produced in a bioengineering facility by the action of enzymes on starch-rich substrates [ 3 , 4 , 5 ]. Naturally occurring cyclodextrins are usually named ‘native CDs’ and include α-CD, β-CD and γ-CD, having six, seven and eight glucose units, respectively.…”