Value of bronchoalveolar lavage and gastric lavage in the diagnosis of pulmonary tuberculosis in children

Somu, N; Swaminathan, Soumya; Paramasivan, C. N.; Vijayasekaran, D; Chandrabhooshanam, A.; Vk, Vijayan; Prabhakar, R

doi:10.1016/s0962-8479(05)80027-9

Cited by 85 publications

(48 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A further seven samples were culture-positive but smear-negative for MTBC organisms; however, five of these were gastric lavage samples. Gastric lavage may be the only useful strategy in attempting the laboratory diagnosis of respiratory tuberculosis in young children but this procedure is unlikely to be helpful in older children and adults [18]. Although it is interesting that one gastric washing sample resulted in a positive test by ProbeTec, we consider that such specimens are, in general, inappropriate for molecular tests.…”

Section: Discussionmentioning

confidence: 89%

An evaluation of the BD ProbeTec ET system for the direct detection of Mycobacterium tuberculosis in respiratory samples

Barrett

Magee

Freeman

2002

Journal of Medical Microbiology

View full text Add to dashboard Cite

In controlling the spread of tuberculosis, early detection of disease caused by organisms of the Mycobacterium tuberculosis complex (MTBC) is vital. The BD ProbeTec ET system provides a method for the direct detection of MTBC by strand displacement amplification. Two hundred and five respiratory samples from patients with a high probability of tuberculosis were assessed by ProbeTec and by microscopy and culture for mycobacteria. ProbeTec positive results were obtained with 101 of 109 samples from which MTBC organisms were isolated. ProbeTec correctly signalled 78 of 81 samples that gave growths of mycobacteria other than tubercle bacilli (MOTT) as negative. Three samples gave false-positive results, corrected on repeat testing. Positive and negative predictive values (PPV, NPV) were 0.97 and 0.90 and the system showed a sensitivity and specificity of 92.7% and 96.0%, respectively. These values rose to PPV 0.97, NPV 0.96, sensitivity 97.1% and specificity 96.0% when data from the small number of gastric lavage samples tested were removed from the analysis. The BD ProbeTec ET system offers a robust and reliable molecular biological approach to the detection of MTBC organisms in respiratory samples in a semi-automated format.

show abstract

Section: Discussionmentioning

confidence: 89%

An evaluation of the BD ProbeTec ET system for the direct detection of Mycobacterium tuberculosis in respiratory samples

Barrett

Magee

Freeman

2002

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…As with intestinal TB samples, untreated-pulmonary-TB samples were positive by fecal PCR, likely due to swallowed M. tuberculosis. Gastric lavage is used widely to diagnose infection in adults and children with pulmonary TB who do not expectorate sputum (15,18). This study suggests that fecal PCR will achieve the same end.…”

mentioning

confidence: 85%

PCR Amplification of the IS 6110 Insertion Element of Mycobacterium tuberculosis in Fecal Samples from Patients with Intestinal Tuberculosis

Balamurugan¹,

Subramanian²,

John

et al. 2006

J Clin Microbiol

View full text Add to dashboard Cite

“…The quantity to be collected was defined through a pilot laboratory test, since this quantity ranges from 5 to 50 mL in the literature. (6,7,14,15) Positive GL samples were defined as those in which Mtb grew in one or more cultures of lavage specimens. Culture results for GL samples collected Contact with an adult index case occurred in almost all cases studied, and the rates were similar in both groups.…”

Section: Resultsmentioning

confidence: 99%

Acurácia do lavado gástrico realizado em ambiente hospitalar e ambulatorial no diagnóstico da tuberculose pulmonar em crianças

et al. 2008

View full text Add to dashboard Cite

Objective: To compare gastric lavage (GL) performed in inpatients with GL performed in outpatients in terms of its accuracy in diagnosing pulmonary tuberculosis (TB) in children. Methods: A prospective study was carried out in the state of Espírito Santo, Brazil, from 1999 to 2003. A total of 230 children suspected of having TB (103 inpatients and 127 outpatients) were selected to undergo GL. Those thus diagnosed with TB (n = 53) were divided into two groups: inpatient GL (n = 30) and outpatient GL (n = 23). All 53 children were monitored for 6 months in order to evaluate the accuracy of the diagnosis. Accuracy was determined based on any change in diagnosis, cure rate, and the percentage of positive cultures in the two groups studied. Results: The cure rate was 100% in both groups, and there was no change in diagnosis in the 53 children studied. No significant difference was found between the two groups studied in terms of detection of Mycobacterium tuberculosis (RR = 1.47; 95% CI: 0.95-2.27; p = 0.095), although the outpatient group presented a greater rate of positive cultures. Conclusions: Our results show that the accuracy of GL performed in an inpatient setting is similar to that of GL performed in an outpatient setting. This indicates that hospitalization is required only in more severe cases in which GL cannot be performed as an outpatient procedure.Keywords: Gastric lavage; Tuberculosis; Diagnosis. ResumoObjetivo: Comparar a acurácia do lavado gástrico (LG) realizado em ambiente hospitalar e ambulatorial no diagnóstico da tuberculose (TB) pulmonar em crianças. Métodos: Estudo retrospectivo realizado no Estado do Espírito Santo, Brasil, de 1999 a 2003. Um total de 230 crianças com suspeita de TB foi selecionado para realizar exame de LG em ambiente hospitalar (n = 103) ou em ambiente ambulatorial (n = 27). Desse total, 53 foram diagnosticadas como casos de TB e divididas em dois grupos: LG hospitalar (n = 30) e LG ambulatorial (n = 23). Todas as 53 crianças foram monitoradas por 6 meses para avaliação da acurácia do diagnóstico. A acurácia foi determinada com base na mudança do diagnóstico, na taxa de cura e no percentual de culturas positivas nos dois grupos estudados. Resultados: A taxa de cura foi de 100% nos dois grupos, e não houve mudança de diagnóstico nas 53 crianças estudadas. Nenhuma diferença significativa foi encontrada entre os dois grupos estudados em relação ao achado do Mycobacterium tuberculosis (RR = 1,47; IC95%: 0,27; p = 0,095), apesar de o grupo LG ambulatorial ter apresentado o maior índice de cultura positivas. Conclusões: Nossos resultados mostram que a acurácia do LG realizado em ambiente hospitalar é semelhante à do realizado em ambiente ambulatorial, o que indica que a internação é necessária apenas em casos mais graves nos quais não se pode realizar o procedimento em ambiente ambulatorial.

show abstract

Value of bronchoalveolar lavage and gastric lavage in the diagnosis of pulmonary tuberculosis in children

Cited by 85 publications

References 11 publications

An evaluation of the BD ProbeTec ET system for the direct detection of Mycobacterium tuberculosis in respiratory samples

An evaluation of the BD ProbeTec ET system for the direct detection of Mycobacterium tuberculosis in respiratory samples

PCR Amplification of the IS 6110 Insertion Element of Mycobacterium tuberculosis in Fecal Samples from Patients with Intestinal Tuberculosis

Acurácia do lavado gástrico realizado em ambiente hospitalar e ambulatorial no diagnóstico da tuberculose pulmonar em crianças

Contact Info

Product

Resources

About