Value of isotypic characterization of antibodies toEchinococcus granulosus by enzyme-linked immuno-filtration assay

Abstract

In order to establish prognostic criteria for patients with hydatid cysts and to identify functional antigenic components of Echinococcus granulosus 87 sera from 36 patients were studied by enzyme-linked immuno-filtration assay (ELIFA) to characterize the four main classes of specific immunoglobulins and to determine compared immunological profiles (CIP) between samples or subjects. This method uses filtration with labelled antibodies to reveal precipitating systems preformed by immuno-electro-diffusion on cellulose acetate strips. IgG antibodies were demonstrated in the arc 5 and in 11 others bands. Specific IgA, IgE and IgM to Echinococcus granulosus was found in 19%, 53% and 69% respectively of patients with hydatids. The presence of IgM and/or IgE antibodies one year after treatment always correlated with disease. Specific IgA was detected more often in patients with pulmonary cysts. Pre- and post-operative monitoring by CIP-ELIFA proved simple and rapid to perform and of decided prognostic value. Two antigens, corresponding to arc 5 and arc X, were frequently associated with the poly-isotypic immune response.

Diagnostic value of a synthetic peptide derived from Echinococcus granulosus recombinant protein.

“…This method has been applied to obtain structural and func- tional information for a large number of proteins (16) The isotypic profile of human antibody response to HCF Ag has been shown to have an interesting prognostic role in hydatidosis (29)(30)(31). According to Dessaint et al (5), a higher concentration of specific IgE antibodies is detected in patients with living E. granulosus cysts.…”

Recent Advances in the Immunology and Serological Diagnosis of Echinococcosis

“…Anti-oncospheral antibodies play a major role in parasite killing and are central to the protective immune response against E. granulosus (Read et al, 2009). In the chronic phase of CE, elevated antibody levels, particularly IgG, IgM and IgE, occur in humans (Khabiri et al, 2006;Daeki et al, 2000;Pinon et al, 1987;Craig, 1986;Dessaint et al, 1975) (Fig. 2), with IgG1 and IgG4 being predominant (Daeki et al, 2000;Sterla et al, 1999;Wen and Craig, 1994).…”

Mechanisms of Immunity in Hydatid Disease: Implications for Vaccine Development

“…As will be described below, these antioncospheral Abs play a major role in parasite killing and are central to the protective immune response against E. granulosus and E. multilocularis. In the chronic phase of CE, elevated levels of Ab, particularly IgG, IgM, and IgE, occur in humans (25)(26)(27)(28)(29) (Fig. 2), with IgG1 and IgG4 being predominant (26,30,31).…”