Valveless, Sealed Microfluidic Device for Automated Heterogeneous Immunoassay: Design and Operational Considerations

Dodge, Arash; Fluri, K.; Linder, Vincent; Lettieri, G.-L.; Lichtenberg, Jan; Verpoorte, Elisabeth; Rooij, N. F. de

doi:10.1007/978-94-017-2264-3_95

Cited by 4 publications

(2 citation statements)

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“…The approach taken in this study sees the immobilization of immunoreagents directly onto microchannel walls, taking advantage of the inherently high surface-to-volume ratio of micrometer-dimensioned channels. − The chamber consists of a microchannel segment nested within a microfluidic network for delivery of samples and reagents. Electrokinetic pumping was explored as a means of fluid propulsion through the channels, due to its suitability for miniaturization with flow rates on a nano- to picoliter per second scale.…”

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confidence: 99%

Electrokinetically Driven Microfluidic Chips with Surface-Modified Chambers for Heterogeneous Immunoassays

et al. 2001

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This article presents the first example of a microfluidic chip for heterogeneous bioassays using a locally immobilized biospecific layer and operated electrokinetically. The reaction chamber has picoliter dimensions and is integrated into a network of microchannels etched in glass. The high affinity of protein A (PA) for rabbit immunoglobulin G (rIgG) was exploited for chip testing, with PA being immobilized on microchannel walls and fluorescently labeled (Cy5) rIgG serving as sample. It was possible to operate the chip in an immunoaffinity chromatographic manner, using electrokinetically pumped solutions. Concentration of antibody from dilute solution onto the solid phase was demonstrated, with signal gains of approximately 30 possible. A dose-response curve for Cy5-rIgG was obtained for concentrations down to 50 nM, for an incubation time of 200 s. The flexibility of chip layout was demonstrated for competitive immunoassay of rIgG, using both a combined sample/tracer incubation and sequential addition of these solutions. With assay times generally below 5 min for this unoptimized device, the microfluidic approach described shows great potential for many high-throughput screening applications.

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Electrokinetically Driven Microfluidic Chips with Surface-Modified Chambers for Heterogeneous Immunoassays

et al. 2001

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“…The photoresist microlenses are fabricated by a resist melting technique [20], [21]. The microchip was designed for biochemical analysis using a heterogeneous immunoassay technique [22]. The microfluidic network is patterned by photolithography and etched with hydrofluoric acid Fig.…”

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confidence: 99%