Vancomycin release from poly(d,l-lactic acid) spray-coated hydroxyapatite fibers

Ravelingien, Matthieu; Mullens, Steven; Luyten, Jan; D׳Hondt, Matthias; Boonen, Jente; Spiegeleer, Bart De; Coenye, Tom; Vervaet, Chris; Remon, Jean Paul

doi:10.1016/j.ejpb.2010.08.010

Cited by 22 publications

(14 citation statements)

References 21 publications

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“…In a recent study on osteomyelitis [26], PDLLA/HA/vancomycin composites showed excellent, sustained antibacterial effects on methicillin-resistant Staphylococcus aureus (MRSA). The PDLLA scaffold containing 28 µm HA fiber coating steadily released up to 23% vancomycin after 28 days.…”

Section: Discussionmentioning

confidence: 99%

Preparation, Characterization, and In Vitro Cytotoxicity Evaluation of a Novel Anti-Tuberculosis Reconstruction Implant

Dong

Zhang

et al. 2014

PLoS ONE

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BackgroundReconstruction materials currently used in clinical for osteoarticular tuberculosis (TB) are unsatisfactory due to a variety of reasons. Rifampicin (RFP) is a well-known and highly effective first-line anti-tuberculosis (anti-TB) drug. Poly-DL-lactide (PDLLA) and nano-hydroxyapatite (nHA) are two promising materials that have been used both for orthopedic reconstruction and as carriers for drug release. In this study we report the development of a novel anti-TB implant for osteoarticular TB reconstruction using a combination of RFP, PDLLA and nHA.MethodsRFP, PDLLA and nHA were used as starting materials to produce a novel anti-TB activity implant by the solvent evaporation method. After manufacture, the implant was characterized and its biodegradation and drug release profile were tested. The in vitro cytotoxicity of the implant was also evaluated in pre-osteoblast MC3T3-E1 cells using multiple methodologies.ResultsA RFP/PDLLA/nHA composite was successfully synthesized using the solvent evaporation method. The composite has a loose and porous structure with evenly distributed pores. The production process was steady and no chemical reaction occurred as proved by Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray Diffraction (XRD). Meanwhile, the composite blocks degraded and released drug for at least 12 weeks. Evaluation of in vitro cytotoxicity in MC3T3-E1 cells verified that the synthesized composite blocks did not affect cell growth and proliferation.ConclusionIt is feasible to manufacture a novel bioactive anti-TB RFP/PDLLA/nHA composite by the solvent evaporation method. The composite blocks showed appropriate properties such as degradation, drug release and biosafety to MC3T3-E1 cells. In conclusion, the novel composite blocks may have great potential for clinical applications in repairing bone defects caused by osteoarticular TB.

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Section: Discussionmentioning

confidence: 99%

Preparation, Characterization, and In Vitro Cytotoxicity Evaluation of a Novel Anti-Tuberculosis Reconstruction Implant

Dong

Zhang

et al. 2014

PLoS ONE

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“…This release profile is recommended to have a complete eradication of the pathogen. 5 The interaction between HA and vancomycin can be explained because HA is mostly negatively charged, 32 and so cationic vancomycin can adsorb onto HA. Collagen is positively charged during vancomycin adsorption (acidic pH), 33 being unlikely that these two molecules interact strongly as they both have the same charge.…”

Section: Discussionmentioning

confidence: 99%

“…4 In a significant number of cases, the prosthesis must be removed and replaced by another, which implies a significant impact in terms of morbidity, mortality, patient discomfort, and medical costs. 5 The recommended treatment for osteomyelitis caused by S. aureus is the long-term parenteral administration of penicillin or vancomycin. In addition, the parenteral administration for this treatment involves the use of catheters, which have their own infection-associated risks and problems.…”

Section: Introductionmentioning

confidence: 99%

Heparinized nanohydroxyapatite/collagen granules for controlled release of vancomycin

Coelho

Sousa

Monteiro

2015

J. Biomed. Mater. Res.

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The purpose of this study was to develop a bone substitute material capable of preventing or treating osteomyelitis through a sustainable release of vancomycin and simultaneously inducing bone regeneration. Porous heparinized nanohydroxyapatite (nanoHA)/collagen granules were characterized using scanning electron microscopy, microcomputed tomography and attenuated total reflectance Fourier transform infrared spectroscopy. After vancomycin adsorption onto the granules, its releasing profile was studied by UV molecular absorption spectroscopy. The heparinized granules presented a more sustainable release over time, in comparison with nonheparinized nanoHA and nanoHA/collagen granules. Vancomycin was released for 360 h and proved to be bioactive until 216 h. Staphylococcus aureus adhesion was higher on granules containing collagen, guiding the bacteria to the material with antibiotic, improving their eradication. Moreover, cytotoxicity of the released vancomycin was assessed using osteoblast cultures, and after 14 days of culture in the presence of vancomycin, cells were able to remain viable, increasing their metabolic activity and colonizing the granules, as observed by scanning electron microscopy and confocal laser scanning microscopy. These findings suggest that heparinized nanoHA/collagen granules are a promising material to improve the treatment of osteomyelitis, as they are capable of releasing vancomycin, eliminating the bacteria, and presented morphological and chemical characteristics to induce bone regeneration.

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“…By combination of different coating thicknesses, the total release profile in vitro can be tuned as a function of the time. [16] useable scaffold for bone regeneration by a tissue engineering approach. Nevertheless the porous scaffold is only one element in a very complex procedure.…”

Section: Surface Modificationmentioning

confidence: 99%

Bone Engineering with Porous Ceramics and Metals

et al. 2011

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The aging of the population of the western countries increasingly necessitate the development of innovative health care approaches including bone replacement and regeneration. The demand for implants is not only intensified but due to the limited life time of these components, an increasing number of implants need revision. Despite the strong research efforts on biomaterials in general, large bone effects are still a big challenge for orthopedic surgeons. Conventionally, bone replacement can be solved by distraction, the use of auto-and allografts or synthetic produced implants. Nevertheless, over the last 20 years intensive research has been performed on a more elegant but more complex new approach called bone tissue engineering. Tissue engineering uses porous scaffolds with properties comparable to trabecular bone. Bone healing is enhanced by seeding these supports with human cells and by the addition of bone growth stimulating molecules. [1] Despite the very successful results obtained on twodimensional materials, it is still waiting on a real reproducible results on three-dimensional scaffolds. A wide variety of materials including ceramics, glass, metals, polymers and composites have been studied to be applied as a porous scaffold material.The self-healing capacity of bone for the reconstruction of small fractures is widely recognized. However, large bone defects still pose a big challenge for orthopedic surgeons. Tissue engineering using porous scaffolds is a new trend to solve this problem and to speed the healing process. When seeded with human cells and bone growth stimulating molecules, they enhance bone healing. Such porous supports try to mimic the properties of trabecular bone. The needed structural properties are: a high and interconnected porosity, pore sizes in the range of 100-500 mm, mechanical properties comparable to trabecular bone and a microporous surface to allow further coating, cell attachment and seeding. Starting from these requirements, it is clear that a cellular material has the ideal architecture for such biomedical scaffold. Polymeric, ceramic or metallic foam structures can be obtained by different manufacturing routes. In our group we optimized three methods, all starting from a powder suspension. They are the PU template technique, gelcasting and the 3D fiber deposition (3DFD) method. Ti and Ti-6Al-4V scaffolds produced by gelcasting and 3DFD can mimic the structural and mechanical properties of the trabecular bone. Hydroxyapatite (HA) and mixtures of hydroxyapatite and beta tricalcium phosphate (b-TCP) were tested for their rate of dissolution in simulated body fluid (SBF) and for their strength as function of their composition. Recently, it was proven that magnesium is a biocompatible and bio-absorbable material. Therefore, as a metallic scaffold it can be expected that it can fulfill both functions of the biomedical scaffold: to be load-bearing and to be bio-absorbable. Nevertheless, the realization of this ideal scaffold needs much more fundamental research. Some preliminar...

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Vancomycin release from poly(d,l-lactic acid) spray-coated hydroxyapatite fibers

Cited by 22 publications

References 21 publications

Preparation, Characterization, and In Vitro Cytotoxicity Evaluation of a Novel Anti-Tuberculosis Reconstruction Implant

Preparation, Characterization, and In Vitro Cytotoxicity Evaluation of a Novel Anti-Tuberculosis Reconstruction Implant

Heparinized nanohydroxyapatite/collagen granules for controlled release of vancomycin

Bone Engineering with Porous Ceramics and Metals

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