Variabilidad morfo-agronómica en genotipos de arroz en el Pacífico Central, Costa Rica

Zúñiga-Orozco, Andrés; González, Ayerin Carrodeguas

doi:10.19053/01228420.v19.n1.2022.12567

Cited by 3 publications

References 13 publications

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Evaluación de cultivares de arroz (Oryza sativa L.) de Vietnam, para su introducción en Cuba

Pérez León,

Díaz López,

Rodríguez Díaz

et al. 2023

Rev. colomb. biotecnol.

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Con el objetivo de evaluar la diversidad fenotípica de ocho cultivares de arroz (Oryza sativa L.) procedentes de Vietnam, para su introducción en los programas de mejora y en la producción en Cuba, apoyados en caracteres morfoagronómicos y técnicas de análisis multivariado, fue desarrollado este trabajo en la Unidad Científico Tecnológica de Base “Los Palacios”, perteneciente al Instituto Nacional de Ciencias Agrícolas de Cuba. Los cultivares vietnamitas se sembraron en campo junto a los comerciales INCA LP-5 e INCA LP-7, para ser caracterizados agronómicamente con la utilización de 29 descriptores cualitativos y 22 cuantitativos. Los resultados mostraron que 16 caracteres cualitativos resultaron homogéneos para los ocho cultivares procedentes de Vietnam y los dos cultivares comerciales cubanos, la asociación entre varios caracteres cuantitativos reafirma la influencia que ejercen algunos componentes sobre el rendimiento, los que son considerados como marcadores para la selección, tres cultivares de procedencia vietnamita (GL301, LCG3-4 y DS1) no pueden ser utilizados en la producción arrocera en Cuba, por su susceptibilidad a Tagosodes. Todos los cultivares mostraron resistencia a Rhizoctonia solani y Sarocladium oryzae y dos (GL 301 y LTH 31) fueron susceptibles a Pyricularia grisea. Los cultivares OM6976, OM5451 y OM8087 alcanzaron valores altos de rendimiento, por lo que pudieran ser utilizados, como progenitores en los programas de mejora, así como cultivares comerciales.

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Evaluación de cultivares de arroz (Oryza sativa L.) de Vietnam, para su introducción en Cuba

Pérez León,

Díaz López,

Rodríguez Díaz

et al. 2023

Rev. colomb. biotecnol.

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Tolerance to aryloxy-phenoxy-propionate (APP) as a model for Lazarroz FL rice in vitro gamma irradiation variability selection

Hernández-Soto

Méndez-Navarro

Pérez

et al. 2022

Preprint

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In vitro gamma ray (60Co) mutagenesis is a powerful tool to achieve variability in commercial rice lines used by farmers, such as Lazarroz FL. We previously reported the optimized in vitro gamma mutagenesis system for Lazarroz FL Indica callus. As a continuation, in the present study, we targeted the ACC2 gene mutagenesis that provides tolerance to aryloxy-phenoxy-propionate (APP) fluazifop-P-butyl as a model to show the system's potential to create variability while providing a solution for weed management. The DL50 of fluazifop-P-butyl was calculated in calli as DL50 = 6,93 mg/L (0,425 mg/L − 15,743 mg/L, R2 = 0,402, 1000n) and regenerated vitroplants at an LD50 of 3.771 mg/L (R2 = 1, 290n). We used 5 mg/L fluazifop-P-butyl as a selection agent and the second round of selection of 10 mg/L (3000 vitroplants) resulted in one survivor plant when using calli as a starting material. The putative tolerant plant also tolerated 150 mg/L in the greenhouse. The ACC2 gene was sequenced, and a heteroecious mutation, T2222I/T2222M, was discovered that may be linked to tolerance. We improved the in vitro system by using seeds as a gamma irradiation starting point instead of embryogenic calli, followed by calli induction, regeneration, and exposure to the selection agent. The modification allowed higher gamma doses with an LD50 of 350 Gy and one to thirty-one putative tolerant plants. The in vitro model showed that gamma-ray mutants from recalcitrant indica rice materials are possible, and the use of selection agents such APP can help create variability useful for breeding a more resilient rice.

show abstract

Tolerance to aryloxy-phenoxy-propionate (APP) as a model for Lazarroz FL rice in vitro gamma irradiation variability selection

Hernández-Soto

Méndez-Navarro

Pérez

et al. 2022

Preprint

View full text Add to dashboard Cite

In vitro gamma ray (60Co) mutagenesis is a powerful tool to achieve variability in commercial rice lines used by farmers, such as Lazarroz FL. We previously reported the optimized in vitro gamma mutagenesis system for Lazarroz FL Indica callus. As a continuation, in the present study, we targeted the ACC2 gene mutagenesis that provides tolerance to aryloxy-phenoxy-propionate (APP) fluazifop-P-butyl as a model to show the system's potential to create variability while providing a solution for weed management. The DL50 of fluazifop-P-butyl was calculated in calli as DL50= 6,93 mg/L (0,425 mg/L - 15,743 mg/L, R2 = 0,402, 1000n) and regenerated vitroplants at an LD50 of 3.771 mg/L (R2 = 1, 290n). We used 5 mg/L fluazifop-P-butyl as a selection agent and the second round of selection of 10 mg/L (3000 vitroplants) resulted in one survivor plant when using calli as a starting material. The putative tolerant plant also tolerated 150 mg/L in the greenhouse. The ACC2gene was sequenced, and a heteroecious mutation, T2222I/T2222M, was discovered that may be linked to tolerance. We improved the in vitro system by using seeds as a gamma irradiation starting point instead of embryogenic calli, followed by calli induction, regeneration, and exposure to the selection agent. The modification allowed higher gamma doses with an LD50 of 350 Gy and one to thirty-one putative tolerant plants. The in vitro model showed that gamma-ray mutants from recalcitrant indica rice materials are possible, and the use of selection agents such APP can help create variability useful for breeding a more resilient rice.

show abstract

Variabilidad morfo-agronómica en genotipos de arroz en el Pacífico Central, Costa Rica

Cited by 3 publications

References 13 publications

Evaluación de cultivares de arroz (Oryza sativa L.) de Vietnam, para su introducción en Cuba

Evaluación de cultivares de arroz (Oryza sativa L.) de Vietnam, para su introducción en Cuba

Tolerance to aryloxy-phenoxy-propionate (APP) as a model for Lazarroz FL rice in vitro gamma irradiation variability selection

Tolerance to aryloxy-phenoxy-propionate (APP) as a model for Lazarroz FL rice in vitro gamma irradiation variability selection

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