Variability in nuclear DNA content within pigeonpea,Cajanus cajan (Fabaceae)

Ohri, Deepak; Jha, Shweta; Kumar, Sudarshan

doi:10.1007/bf00939727

Cited by 9 publications

(6 citation statements)

References 14 publications

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“…The similarity coefficient of accession 8081 with other accessions ranges from 0.394 to 0.467 while among other accessions it varies between 0.760 and 1.0 (Table 2). This variation is in accordance with 23.6% difference in 4C DNA amounts in 16 accessions (Ohri et al, 1994), besides morphological and phenological variations. Among the 10 species studied, the maximum (27) number of bands were present in C. goensis while C. volubilis showed minimum (13) number of bands.…”

Section: Resultssupporting

confidence: 63%

“…Cytologically, the chromosome homology expressed in successful crosses involving C. cajan with other wild species was no less than that expressed in crosses amongst the wild Cajanus species (Pundir & Singh, 1985c). The 4C DNA amounts of C. cajan and 7 other species of Cajanus are also in accordance with the crossability relationships as C. cajanifolius, C. goensis, C. lineatus and C. scarabaeoides (range 6.93-8.08 pg) have almost similar values as those of C. cajan (6.19-7.97 pg), while those of C. platycarpus (5.18 pg), C_ volubilis (4.65 pg) and C. acutifolius (11_69 pg) are highly divergent (Ohri et al, 1994;Ohri & Jha, unpubl.). Pundir and Singh (1985a) have suggested three possible pathways for the origin and evolution of cultivated pigeonpeas which might be through selection of gene mutations in C. cajanifolius, or the cultigen along with other wild species has evolved from a common gene pool, or it might be a polyphyletic origin by the selection of erect vigorous plants from an interspecific cross involving two such species as C. lineatus and C. scarabaeoides.…”

Section: Figsupporting

confidence: 67%

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Phylogenetic relationships of Cajanus cajan (L.) Millsp. (pigeonpea) and its wild relatives based on seed protein profiles

Jha

Ohri

1996

Genet Resour Crop Evol

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Total seed storage protein of 9 accessions of cultivated C. cajan and 10 wild Cajanus species was reported and compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis. A considerable variation was detected among the protein profiles of different accessions of C. cajan while those of wild species were very specific and distinctly different from each other. Relative similarities between various taxa were estimated by Jaccard's similarity index and cluster analysis was performed to produce a UPGMA dendrogram. The clustering of 10 wild species and C. cajan more or less agrees with their sectional classification and available data based on morphological characteristics, crossability, genome pairing in hybrids and nuclear RFLPs. The species closest to C. cajan is C. cajanifolia although the accessions of C. ca jan also share some bands present in the profiles of C. scarabaeoides, C. goensis, C. lineatus, C. acutifolius and C. volubilis. This points towards polyphyletic origin of the cultigen which has been discussed in this paper.

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Section: Resultssupporting

confidence: 63%

Section: Figsupporting

confidence: 67%

Phylogenetic relationships of Cajanus cajan (L.) Millsp. (pigeonpea) and its wild relatives based on seed protein profiles

Jha

Ohri

1996

Genet Resour Crop Evol

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“…Studies based on morphology (van der Maesen 1980(van der Maesen , 1986(van der Maesen , 1990, cytology and crossability (Pundir and Singh 1985a, b), isozymes (Krishna and Reddy 1982) and nuclear RFLPs (Nadimpalli et al 1993) suggest a mono-phyletic origin from C. cajanifolius. On the other hand, the seed storage protein profiles (Ladizinsky and Hamel 1980;Jha and Ohri 1996) and nuclear DNA amounts (Ohri et al 1994) suggest a polyphyletic origin of the cultigen.…”

Section: Introductionmentioning

confidence: 97%

Genomic relationships among 11 species in the genus Cajanus as revealed by seed protein (albumin and globulin) polymorphisms

Panigrahi¹,

Kumar²,

Mishra³

et al. 2007

Plant Biotechnol Rep

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SDS-PAGE analysis of seed albumins and globulins from two pigeonpea, Cajanus cajan, cultivars (DSLR-17 and BDN-2) and ten wild species, including C. cajanifolius, C. lineatus, C. sericeus, C. acutifolius, C. lanceolatus, C. reticulates, C. albicans, C. scarabaeoides, C. volubilis and C. platycarpus, resulted in 34 albumin and 27 globulin polypeptides. Proximity matrix analysis based on electrophoretic banding patterns of albumins and globulins jointly revealed C. cajanifolius to be closest to C. cajan having similarity coefficients of 0.595 and 0.676, respectively. Cluster analysis also exhibited the grouping of C. cajanifolius with C. cajan in one cluster. C. platycarpus has been out grouped. The present analysis more or less agreed with the sectional classification of the genus Cajanus and it has been hypothesized that cultivated pigeonpea has evolved through multi-genomic interaction involving C. cajanifolius and that it has experienced minor genomic reorganization during its divergence.

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“…Again in the present study, C. platycarpus is out grouped and justified its status in the tertiary gene pool [3]. However, the results of present study were at variance with crossability relationships and DNA content studies [8,14], and it might be due to the genetic divergence during the course of evolution under selection pressure. The evidences obtained from different studies till now, including the present study, unequivocally support C. cajanifolius to be the closest relative of cultivated pigeonpea [3,4,6,7,[9][10][11]13].…”

Section: Discussionmentioning

confidence: 45%

“…Studies on morphology [3,4,6], cytology and crossability [7,8], isozymes [9] and nuclear RFLPs [10], suggested a monophyletic origin from C. cajanifolius. On the other hand, studies on seed protein profiling [11][12][13] and nuclear DNA amounts [14], suggested a polyphyletic origin of the C. cajan.…”

Section: Introductionmentioning

confidence: 99%

Elucidation of Genetic Relationships in the Genus Cajanus Using Random Amplified Polymorphic DNA Marker Analysis

Mishra¹

2013

J Phylogen Evolution Biol

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In this present investigation, a total of 27 RAPD primers were used to elucidate the genetic relationships between cultivated Cajanus cajan cultivars and 10 allied species of primary, secondary and tertiary gene pool, including C. cajanifolius, C. scarabaeoides, C. platycarpus, C. albicans, C. volubilis, C. sericeus, C. acutifolius, C. lineatus, C. lanceolatus and C. reticulates. All primers showed polymorphism at species level and produced 215 unequivocal polymorphic bands, with an average of 7.96 bands per primer. These polymorphic primers exhibited variation with regard to average band informativeness, resolving power, and showed high polymorphism information content value. No single primer was able to distinguish between all the two cultivars of C. cajan and ten allied species of Cajanus, but several species specific amplified fragments were observed. The pair wise Jaccard's similarity coefficient values revealed high level of inter-specific genetic variation in the genus Cajanus. Cluster analysis exhibited the grouping of two C. cajan accessions with C. cajanifolius in one cluster, while except C. platycarpus, all the nine wild Cajanus species belonging to the secondary and tertiary gene pool form another cluster. The present analysis more or less agreed with the sectional classification of the genus Cajanus, and it has been hypothesized that cultivated pigeonpea has evolved through multi-genomic interaction through C. cajanifolius, and it has experienced minor genomic reorganization during its divergence. Again, identification of species specific amplification pattern substantiated the utility of RAPD markers on selection of suitable species to transfer the desirable trait into cultivated C. cajan, through marker aided breeding for its genetic augmentation, and also for the effective management of genetic resources of C. cajan.

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Variability in nuclear DNA content within pigeonpea,Cajanus cajan (Fabaceae)

Cited by 9 publications

References 14 publications

Phylogenetic relationships of Cajanus cajan (L.) Millsp. (pigeonpea) and its wild relatives based on seed protein profiles

Phylogenetic relationships of Cajanus cajan (L.) Millsp. (pigeonpea) and its wild relatives based on seed protein profiles

Genomic relationships among 11 species in the genus Cajanus as revealed by seed protein (albumin and globulin) polymorphisms

Elucidation of Genetic Relationships in the Genus Cajanus Using Random Amplified Polymorphic DNA Marker Analysis

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