Variability of <i>Pyrenophora tritici-repentis</i> isolated from different wheat areas of Tunisia: Morpho-cultural characterization, pathogenic analysis and virulence effector genes

Tissaoui, Salma; Mougou‐Hamdane, Amira; Omri-Benyoussef, Noura; Nasraoui, B.

doi:10.1080/03235408.2021.1991127

Cited by 3 publications

(4 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two weeks old, dark colonies usually form black spherical aggregations in the medium (Hosford, 1971;Benslimane et al 2017). Results of this study revealed that morphological traits of Ptr have a similar range of variation to isolates from Tunisia (Tissaoui et al 2021) and had wider variation than previously reported by Hosford (1971) and Benslimane et al (2017) and. This diversity of phenotypes could also indicate genotypic diversity of the pathogen; however, a clear connection between these characteristics has not been found for Ptr.…”

Section: Discussionsupporting

confidence: 67%

Diversity in morphotypes and necrotrophic effectors (Nes) of Pyrenophora tritici-repentis strains in Latvia and Belarus

Kaņeps

Moročko‐Bičevska

Bankina

et al. 2022

CEREAL RESEARCH COMMUNICATIONS

View full text Add to dashboard Cite

Pyrenophora tritici-repentis (Ptr), family Pleosporaceae, is a common wheat pathogen in all wheat-growing regions around the globe. It is widely studied in North America, South America, and North Africa, while data about the fungus genetic diversity in Europe is still insufficient. This study aimed to describe the variation of morphological traits and toxin production of strains collected in Latvia and Belarus. Twenty-one isolates from Latvia, and 12 from Belarus were sampled in 2019 for morphological evaluation in culture and necrotrophic effector gene determination by PCR. All isolates were grouped into nine different morphotypes. Five of these morphotypes were unique for isolates from Latvia, one for Belarus, and three morphotypes were occurring in both countries. No association between the host and the pathogen morphotype was observed. ToxA gene was detected in 44% of the analysed isolates. For 52% of the isolates, PCR did not confirm the presence of any known effector genes of Pyrenophora tritici-repentis. ToxB and toxb were found only in one isolate from Latvia. The studies need to be continued to evaluate the diversity of the pathogen depending on the host species.

show abstract

Section: Discussionsupporting

confidence: 67%

Diversity in morphotypes and necrotrophic effectors (Nes) of Pyrenophora tritici-repentis strains in Latvia and Belarus

Kaņeps

Moročko‐Bičevska

Bankina

et al. 2022

CEREAL RESEARCH COMMUNICATIONS

View full text Add to dashboard Cite

show abstract

“…All the strains possess ToxB gene, only two strains possess ToxA gene, and three strains contain the homolog Toxb . These profiles of fungi were identified on investigation of Kamel et al [ 6 ] and Laribi et al [ 36 ] in Tunisia. These five tested strains of Ptr exhibited different levels of virulence on the most cultivated durum wheat varieties used by Tunisian farmers.…”

Section: Discussionmentioning

confidence: 99%

“…The nonionic and polar molecule Ptr ToxC is controlled by the insensitivity gene Tsc 1 which is located in the short arm of chromosome 1A [ 35 ]. In Tunisia, the distinct profile of pathogen effector genes [ 6 , 36 ], and several races were discovered [ 6 , 37 ] in strains collected from different areas of Northern Tunisia. Prevalence, incidence, and severity percentages varied between geographical region and cultivated varieties [ 38 , 39 ] but the response of commercialized wheat varieties to tan spot pathogen, and their resistance level remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Varietal Screening of Durum Wheat Varieties for Resistance to Pyrenophora tritici-repentis (Tan Spot) under Field Conditions

Tissaoui

Hassine

Mougou‐Hamdane

et al. 2022

BioMed Research International

View full text Add to dashboard Cite

Tan spot disease caused by Pyrenophora tritici-repentis was becoming more bred in Tunisia during the last decade. The search for resistant varieties against the increased virulence diversity of P. tritici-repentis is presently considered as a priority. Seven of the most commercialized durum wheat varieties in Tunisia (cvs. Maâli, Salim, Razzak, Monastir, Khiar, Inrat100, and Sculptur) were inoculated with five characterized fungal strains under field conditions, during two seasons. The variance analysis revealed that strains Ech8F6 and B4.8 used in inoculation are the most virulent ones. These strains hosting ToxB gene caused chlorosis symptom on the tested varieties. The other strains induced necrosis with yellow halo and host ToxA gene were less virulent. The area under disease progress curve values revealed that Maâli is the most vulnerable genotype compared to the new selected varieties Monastir and Inrat100. A variable tolerance rate of the varieties to tan spot disease was also highly visible on yield components. The losses were about 22.2% of the thousand kernel weight in Maâli variety, 35% of spikes/m2 in Inrat100 variety, 32.5% of kernel number/spike, and 25.2% of yield grain in Monastir variety. This effect evaluation of the strains harbouring ToxA and ToxB genes could be responsible for the identification of potentially susceptible genes Tsn1 and Tsc2 representing resistance sources for breeding programs.

show abstract

“…In Tunisia, tan spot disease is an emerging threat to wheat production [ 11 , 14 ]. Although no studies were carried out to assess the extent of yield losses, recent studies about Ptr in Tunisia revealed a high morphological, phenotypic, and genotypic diversity [ 19 , 24 , 25 , 39 ]. Six races, namely, 2, 4, 5, 6, 7, and 8, were identified in addition to atypical isolates that were able to cause necrosis on the differential line ‘Glenlea’ but lacked the expected ToxA gene [ 19 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Mediterranean Durum Wheat for Resistance to Pyrenophora tritici-repentis

Laribi

Yahyaoui

Abdedayem

et al. 2022

Genes

View full text Add to dashboard Cite

Tan spot (TS), caused by the fugus Pyrenophora tritici-repentis (Ptr), has gained significant importance in the last few years, thereby representing a threat to wheat production in all major wheat-growing regions, including Tunisia. In this context, we evaluated a Mediterranean collection of 549 durum wheat accessions under field conditions for resistance to Ptr over two cropping seasons in Jendouba (Tunisia), a hot spot for Ptr. The relative disease severities showed significant phenotypic variation from resistance to susceptibility. The correlation between disease scores over the two trials was significant, as 50% of the accessions maintained good levels of resistance (resistant–moderately resistant). Seedling- and adult-stage reactions were significantly correlated. The ANOVA analysis revealed that the genotype term is highly significant at the adult stage, thus emphasizing the high genetic variability of the tested accessions. Reaction-type comparison among and between countries revealed a high diversity of TS resistance. Plant height (PH) was negatively correlated to disease scores, indicating that PH might either have a significant effect on TS severity or that it can be potential disease escape traits. The evaluation of this collection allowed for the identification of potential diverse resistance sources to Ptr that can be incorporated in breeding programs.

show abstract

Variability of Pyrenophora tritici-repentis isolated from different wheat areas of Tunisia: Morpho-cultural characterization, pathogenic analysis and virulence effector genes

Cited by 3 publications

References 37 publications

Diversity in morphotypes and necrotrophic effectors (Nes) of Pyrenophora tritici-repentis strains in Latvia and Belarus

Diversity in morphotypes and necrotrophic effectors (Nes) of Pyrenophora tritici-repentis strains in Latvia and Belarus

Varietal Screening of Durum Wheat Varieties for Resistance to Pyrenophora tritici-repentis (Tan Spot) under Field Conditions

Characterization of Mediterranean Durum Wheat for Resistance to Pyrenophora tritici-repentis

Contact Info

Product

Resources

About