Variability of nuclear and mitochondrial ribosomal DNA of a truffle species (Tuber aestivum)

Guillemaud, Thomas; Raymond, M.; Callot, G.; Cleyet-Marel, Jean-Claude; Fernandez, Diana

doi:10.1016/s0953-7562(96)80007-4

Cited by 22 publications

(21 citation statements)

References 9 publications

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“…The majority (85.2%) of T. aestivum/T. Considering the additional MspI pattern identified in previous studies [3,9], all the ITS T. aestivum/T. Moreover, truffles showing the MspI-2 pattern displayed a further poly-morphism when RFLP analysis was performed with the enzymes TaqI and MseI (Figs.…”

Section: Amplification and Rflp Analysis Of The Itsmentioning

confidence: 73%

“…Length polymorphism was detected between T. aestivum/T. The combination of three restriction enzymes therefore allowed us to discover more ITS/RFLP classes than had been identified previously [3,9]. Fig 1(a)).…”

Section: Amplification and Rflp Analysis Of The Itsmentioning

confidence: 89%

“…Fruit bodies belonging to Tuber aestivum Vittad., Tuber uncinatum Chatin or to the black Asian truffle species Tuber indicum Cooke and Massee and Tuber himalayense Zhang and Minter, generally display a large set of sporal configurations associated with quite pronounced molecular variability [1][2][3][4][5]. Some species belonging to this genus are economically important because they produce edible truffles with a distinctive flavour.…”

Section: Introductionmentioning

confidence: 99%

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Tuber aestivum and Tuber uncinatum: two morphotypes or two species?

Paolocci

Rubini

Riccioni

et al. 2004

FEMS Microbiology Letters

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Tuber spp. are ectomycorrhizal fungi that establish symbioses with shrubs and trees. Because of their different smell and taste, Tuber uncinatum and Tuber aestivum are two truffle morphotypes with a different market value, but whether or not T. uncinatum and T. aestivum are different taxa is still an open debate among mycologists. In order to identify molecular keys characterizing both T. aestivum and T. uncinatum morphotypes, ITS/RFLPs analyses were carried out on a large collection of samples from all over Italy and from other European countries, followed by a study of the phylogenesis of ITS, beta-tubulin and EF 1-alpha genes, on representative samples. The present study provides compelling evidence that: (i) T. uncinatum and T. aestivum belong to the same species, (ii) neither morphotype presents a specific molecular fingerprint, but they may even share identical alleles at any of the loci analysed; (iii) T. aestivum is most likely under a selfing reproductive mode. Our findings suggest that ecological, rather than genetic causes may account for differences in sporal morphology, taste and smell between T. aestivum and T. uncinatum truffles.

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Section: Amplification and Rflp Analysis Of The Itsmentioning

confidence: 73%

Section: Amplification and Rflp Analysis Of The Itsmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Tuber aestivum and Tuber uncinatum: two morphotypes or two species?

Paolocci

Rubini

Riccioni

et al. 2004

FEMS Microbiology Letters

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“…The spatial distribution and ecological requirements of these symbiotic fungi vary by species. Some species are widely distributed and have pronounced morphological and molecular variability (9,11,22,25,27,32), while others have a more restricted distribution and little intraspecific polymorphism in either morphological or genetic traits (3,4,8,9,26). Tuber magnatum Pico, which produces white ascomata, is harvested only in Italy and some countries on the Balkan Peninsula.…”

mentioning

confidence: 99%

Genetic and Phylogeographic Structures of the Symbiotic Fungus Tuber magnatum

Rubini

Paolocci

Riccioni

et al. 2005

Appl Environ Microbiol

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The quality and market price of truffles vary with the species and, traditionally, the place of origin. The premium species Tuber magnatum produces white truffles and has a patchy distribution restricted to Italy and some Balkan areas. We used polymorphic microsatellites to evaluate 316 specimens grouped into 26 populations sampled across the species' geographic range to determine if natural populations of T. magnatum are genetically differentiated. We found that the southernmost and the northwesternmost populations were significantly differentiated from the rest of the populations. The simple sequence repeat data also could be used to make inferences about the postglacial T. magnatum expansion pattern. This study is the first to identify a genetic and phylogeographic structure in T. magnatum. The presence of a genetic structure can be of practical interest in tracing truffle populations according to their geographic origin for marketing strategies. Evidence for extensive outcrossing in field populations of T. magnatum also is provided for the first time.Tuber spp. are hypogeous ascomycetes that grow in ectomycorrhizal symbioses with some shrub and tree species and produce ascomata, known as truffles. Some truffles are edible and appreciated worldwide. Their quality and market price depend on the species and, traditionally, the place of origin. Natural truffle production has declined dramatically over the past century (12). This decline and the flourishing truffle market have encouraged large-scale programs for growing these fungi through the planting of nursery-produced mycorrhizal trees. These programs have been widely developed for some species, e.g., Tuber melanosporum Vittad. and Tuber uncinatum Chatin, and most of the commercial demand for these truffles is satisfied by these artificial plantations rather than natural field collections (12). How the deliberate introduction of foreign strains into native populations has affected truffle production and biodiversity remains unknown.The complexity of the truffle life cycle, the difficulties of growing these fungi under controlled conditions, and the lack of reliable phenotypic markers to differentiate morphologically similar species have been major obstacles to understanding the distribution, propagation, and fructification of these hypogeous fungi. Molecular markers have been developed to type most of the economically important truffle species (2,10,15,19,23,24,25,33,34), but studies of the environmental and molecular determinants for different portions of the life cycle generally are lacking. The spatial distribution and ecological requirements of these symbiotic fungi vary by species. Some species are widely distributed and have pronounced morphological and molecular variability (9,11,22,25,27,32), while others have a more restricted distribution and little intraspecific polymorphism in either morphological or genetic traits (3,4,8,9,26). Tuber magnatum Pico, which produces white ascomata, is harvested only in Italy and some countries on the Balkan Peninsul...

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“…Except for a handful of studies on particular genera (Gardes, et al 1990, Agerer, Kraigher & Javornik 1996, Hibbett & Donoghue 1996, Farnet, Roux & Le Petit 1999, Gomes et al 1999, Vasiliauskas, Johanneson & Stenlid 1999 there has been little work across most broad groups of larger basidomycete fungi to test this assumption. Likewise localised variation has been examined in very few ascomycete ectomycorrhizal fungi (Guillemaud et al 1996, Kagan-zur 1998. While the situation in the Northern Hemisphere, and possibly even the eastern part of Australia , may be quite different, it is apparent that intraspecific variation in the ITS is sufficiently common in Western Australian fungi to necessitate the examination of more than one collection of a species when conducting molecular investigations, whether for community studies or phylogenetic analysis.…”

Section: Significance For Interpreting the Ecology Of Eucalyptus Ecosmentioning

confidence: 99%

Interspecific and intraspecific variation of ectomycorrhizal fungi associated with Eucalyptus ecosystems as revealed by ribosomal DNA PCR-RFLP

Glen

Tommerup

Bougher

et al. 2001

Mycological Research

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Gondwanan vegetation, and the Australian region in particular, is species rich for ectomycorrhizal fungi in epigeous and hypogeous forms with over 100 species recorded in small (1 ha) patches of forests. Distinguishing co-occurring ectomycorrhizal fungi as root associations in native (natural or wildlands) vegetation or plantations and discriminating them from other larger basidiomycetes, e.g. wood and leaf litter decomposer fungi, places large demands on molecular identification, especially if interspecific similarities and intraspecific variation occur in target sequences. One hundred and nine species of larger basidiomycetes from a single forest location were characterised by PCR-RFLP profiles of two genomic regions (nuclear rDNA ITS and mtLSU). Over one-third of the species for which multiple isolates were tested showed intraspecific variation in either one or both genomic regions. This remarkably high variation questions previous assumptions about intraspecific ITS sequence variation and highlights the value of integrated molecular and morphological databases including voucher specimens. It also emphasises the value of molecular investigations that use more than one genomic region. Interspecific similarities were common among the Cortinariaceae, especially in the ITS region. Discrimination of most Cortinariaceae species was achieved using variation in the mtLSU region in conjunction with the ITS. This new information raises the possibilities that the ITS sequence is more conserved and the mtLSU more variable than among species of the other 23 families. In the other families, interspecific ITS variation was greater and the mtLSU profiles grouped species within families. The high variation in the two genomic regions indicated possible differences in the fungal population structure between two adjacent, differently managed blocks of Eucalyptus marginata forest. The significance of this variation to ecology, biodiversity assessment and ecosystem management are discussed.

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Variability of nuclear and mitochondrial ribosomal DNA of a truffle species (Tuber aestivum)

Cited by 22 publications

References 9 publications

Tuber aestivum and Tuber uncinatum: two morphotypes or two species?

Tuber aestivum and Tuber uncinatum: two morphotypes or two species?

Genetic and Phylogeographic Structures of the Symbiotic Fungus Tuber magnatum

Interspecific and intraspecific variation of ectomycorrhizal fungi associated with Eucalyptus ecosystems as revealed by ribosomal DNA PCR-RFLP

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