Variability of partial pressures of CO2 and O2 in resting skeletal muscle

Liew, Hugh D. Van; Rodgers, Barbara J.

doi:10.1016/0026-2862(75)90001-1

Cited by 5 publications

(2 citation statements)

References 21 publications

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“…Values for tissue p o p in resting skeletal muscle reported in the literature show a large distribution, partly due to the technical difficulties of measuring tissue PO, accurately. Estimates in the range of 10-30 mmHg seem, however, to be most frequent (Whalen et al 1974, Van Liew et al 1975, Weiss et al 1976. During exposure of the left tenuissimus muscle to atmospheric oxygen tension (150 mmHg), the total blood flow was 0.7f0.4 ml min-' .…”

Section: Resultsmentioning

confidence: 95%

Blood flow in the rabbit tenuissimus muscle Influence of preparative procedures for intravital microscopic observation

Lindbom¹,

Tuma²,

Arfors³

1982

Acta Physiologica Scandinavica

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The tenuissimus muscle in the rabbit and the cat is a suitable tissue for intravital microscopic investigation of skeletal muscle blood flow. In this study the influence of surgical procedures necessary for direct microscopic observation on the physiological state of the rabbit tenuissimus muscle was assessed by means of blood flow measurements. Mean resting blood flow was 2.8 +/0 0.8 (mean +2- S.D.) ml.min-1.100 g-1 in the left tenuissimus muscle when prepared for microscopic observation as determined by the radioactive microsphere method. This value was not significantly different from that in the intact unexposed muscle in the contralateral leg, 3.3 +/- 1.1. ml.min-1.100 g-1. Exposure of the muscle to atmospheric oxygen tension resulted in a reduction of blood flow to 0.7 +/- 0.4 ml.min-1.100 g -1, suggesting that local metabolic control mechanisms were active. The normal range of vascular control seemed to be maintained, as demonstrated by an increase in blood flow to 64.2 +/- 18.8 ml.min-1.100 g-1 during "maximal" vasodilation induced by topical application of PGE1. The tenuissimus muscle showed a marked sensitivity to mechanical stimulation. Slight stretching of the muscle, similar to what may occur during surgical preparation, resulted in an increase in blood flow to 17.5 +/- 5.7 ml.min-1.100 g-1. Flow values calculated from data obtained by direct microscopic measurements in the tenuissimus muscle agreed well with those obtained by the microsphere method.

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Section: Resultsmentioning

confidence: 95%

Blood flow in the rabbit tenuissimus muscle Influence of preparative procedures for intravital microscopic observation

Lindbom¹,

Tuma²,

Arfors³

1982

Acta Physiologica Scandinavica

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“…Similarly a spatial heterogeneity of car bon dioxide in the extracellular space was described [4][5][6], Extracellular skeletal muscle tissue pH (pH,) distributions were examined in vitro using pH microelectrodes, and a spatial variability with values ranging from 7.0 to 7.4 was observed [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

pH Heterogeneity in Skeletal Muscle Extracellular Fluid

1996

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Oxygen and carbon dioxide are known to be heterogeneously distributed in tissues. Extracellular skeletal muscle tissue pH (pH_t) also exhibits a spatial variability in vitro, but this has not been examined in vivo. pH_t distributions in resting skeletal muscle and the effect of the dispersion of pH_t on ischemia and normoxic hypercarbia was therefore studied in an animal model with a multichannel pH microelectrode. Under resting conditions and spontaneous breathing, local pH_t (from all animals, n = 10) was found to vary between 6.96 and 7.68 (range), and 70% of the values were within a pH of 7.00-7.32. In each animal the maximum pH_t differences (maximum range between the 6 channels of the microelectrode) found were 0.32 ± 0.11 pH units (mean ± SD). During tissue acidosis, induced by ischemia, no significant change in the local pH_t differences in each animal was seen. During normoxic hypercarbia a 2-fold increase in pH_t variability within each animal was noticed (p < 0.01), which suggests that carbon dioxide and buffering effects of the blood are significant factors for the pH_t distribution. The pH_t distribution range found is of similar magnitude as previously described in in vitro studies on skeletal muscle. Locally varying pH_t levels may be of importance as they will affect cellular H⁺ extrusion, membrane potential and volume control of different cell populations differently.

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Effect of Local Metabolic Factors on Vascular Smooth Muscle

Sparks

1980

Comprehensive Physiology

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Variability of partial pressures of CO2 and O2 in resting skeletal muscle

Cited by 5 publications

References 21 publications

Blood flow in the rabbit tenuissimus muscle Influence of preparative procedures for intravital microscopic observation

Blood flow in the rabbit tenuissimus muscle Influence of preparative procedures for intravital microscopic observation

pH Heterogeneity in Skeletal Muscle Extracellular Fluid

Effect of Local Metabolic Factors on Vascular Smooth Muscle

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