Variability of the salivary proteins of 20 Brazilian populations of Panstrongylus megistus (Hemiptera: Reduviidae: Triatominae)

Barbosa, Silvia Ermelinda; Diotaiuti, Liléia; Braga, Érika Martins; Pereira, Marcos H.

doi:10.1016/j.actatropica.2004.05.012

Cited by 16 publications

(18 citation statements)

References 27 publications

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“…A similar band was also present in P. lutzi hemolymph, confirming that the genus Panstrongylus has some homogeneity [77] although considerable variation in salivary gland proteins has been shown even within P. megistus populations [78]. Recently, a salivary gland serine protease has been described in P. megistus [79,80], as well as Kazal-type and lipocalin inhibitors.…”

Section: Discussionmentioning

confidence: 76%

“…Nevertheless, work on P megistus is in its infancy with only 45 ESTs reported recently as available for this species [64] so that the detection of many new factors, such as the serpin in the stomach, fat body and hemocytes of the present paper, is to be expected. The fact that serpins have not been reported from other triatomine species, except for only a truncated cDNA cluster in the sialome of Triatoma infestans [31], may reflect the more recent evolution of this vector to the hematophagous habit and the need to adapt to changes in diet [78]. …”

Section: Discussionmentioning

confidence: 99%

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Isolation and molecular characterization of a major hemolymph serpin from the triatomine, Panstrongylus megistus

et al. 2014

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BackgroundChagas disease kills 2.5 thousand people per year of 15 million persons infected in Latin America. The disease is caused by the protozoan, Trypanosome cruzi, and vectored by triatomine insects, including Panstrongylus megistus, an important vector in Brazil. Medicines treating Chagas disease have unpleasant side effects and may be ineffective, therefore, alternative control techniques are required. Knowledge of the T. cruzi interactions with the triatomine host needs extending and new targets/strategies for control identified. Serine and cysteine peptidases play vital roles in protozoan life cycles including invasion and entry of T. cruzi into host cells. Peptidase inhibitors are, therefore, promising targets for disease control.MethodsSDS PAGE and chromatograpy detected and isolated a P. megistus serpin which was peptide sequenced by mass spectrometry. A full amino acid sequence was obtained from the cDNA and compared with other insect serpins. Reverse transcription PCR analysis measured serpin transcripts of P. megistus tissues with and without T. cruzi infection. Serpin homology modeling used the Swiss Model and Swiss-PDB viewer programmes.ResultsThe P. megistus serpin (PMSRP1) has a ca. 40 kDa molecular mass with 404 amino acid residues. A reactive site loop contains a highly conserved hinge region but, based on sequence alignment, the normal cleavage site for serine proteases at P1-P1′ was translocated to the putative position P4′-P5′. A small peptide obtained corresponded to the C-terminal 40 amino acid region. The secondary structure of PMSRP1 indicated nine α-helices and three β-sheets, similar to other serpins. PMSRP1 transcripts occurred in all tested tissues but were highest in the fat body and hemocytes. Levels of mRNA encoding PMSRP1 were significantly modulated in the hemocytes and stomach by T. cruzi infection indicating a role for PMSRP1 in the parasite interactions with P. megistus.ConclusionsFor the first time, a constitutively expressed serpin has been characterized from the hemolymph of a triatomine. This opens up new research avenues into the roles of serine peptidases in the T. cruzi/P. megistus association. Initial experiments indicate a role for PMSRP1 in T. cruzi interactions with P. megistus and will lead to further functional studies of this molecule.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Isolation and molecular characterization of a major hemolymph serpin from the triatomine, Panstrongylus megistus

et al. 2014

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“…Saliva of hematophagous species can differ in its composition between populations of the same species such as analyzed for sand flies [44] and triatomines [37], [45], [46]. Additionally, salivary proteins of arthropods vary not only between populations but also between developmental stages [47], [48].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Guinea Pig Antibody Responses to Salivary Proteins of Triatoma infestans for the Development of a Triatomine Exposure Marker

et al. 2014

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BackgroundSalivary proteins of Triatoma infestans elicit humoral immune responses in their vertebrate hosts. These immune responses indicate exposure to triatomines and thus can be a useful epidemiological tool to estimate triatomine infestation. In the present study, we analyzed antibody responses of guinea pigs to salivary antigens of different developmental stages of four T. infestans strains originating from domestic and/or peridomestic habitats in Argentina, Bolivia, Chile and Peru. We aimed to identify developmental stage- and strain-specific salivary antigens as potential markers of T. infestans exposure.Methodology and Principal FindingsIn SDS-PAGE analysis of salivary proteins of T. infestans the banding pattern differed between developmental stages and strains of triatomines. Phenograms constructed from the salivary profiles separated nymphal instars, especially the 5th instar, from adults. To analyze the influence of stage- and strain-specific differences in T. infestans saliva on the antibody response of guinea pigs, twenty-one guinea pigs were exposed to 5th instar nymphs and/or adults of different T. infestans strains. Western blot analyses using sera of exposed guinea pigs revealed stage- and strain-specific variations in the humoral response of animals. In total, 27 and 17 different salivary proteins reacted with guinea pig sera using IgG and IgM antibodies, respectively. Despite all variations of recognized salivary antigens, an antigen of 35 kDa reacted with sera of almost all challenged guinea pigs.ConclusionSalivary antigens are increasingly considered as an epidemiological tool to measure exposure to hematophagous arthropods, but developmental stage- and strain-specific variations in the saliva composition and the respective differences of immunogenicity are often neglected. Thus, the development of a triatomine exposure marker for surveillance studies after triatomine control campaigns requires detailed investigations. Our study resulted in the identification of a potential antigen as useful marker of T. infestans exposure.

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“…As pesquisas de composição da saliva, durações do ciclo biológico, tamanho dos ovos, variabilidade genética e morfologia da genitália masculina de populações de P. megistus reforçaram a hipótese de diferenças entre as populações do sul, sudeste e nordeste (BARBOSA et al, 1999(BARBOSA et al, , 2001(BARBOSA et al, , 2003(BARBOSA et al, , 2004(BARBOSA et al, , 2006. P. megistus apresenta boa capacidade de invasão de ecótopos artificiais e grande potencial de domiciliação e ainda uma alta taxa de infecção natural por T.…”

Section: Aderido 157unclassified

Caracterização de resistência a inseticidas em populações da subfamilia Triatominae (Hemiptera:Reduviidae), vetores de Trypanosoma cruzi Chagas, 1909

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Variability of the salivary proteins of 20 Brazilian populations of Panstrongylus megistus (Hemiptera: Reduviidae: Triatominae)

Cited by 16 publications

References 27 publications

Isolation and molecular characterization of a major hemolymph serpin from the triatomine, Panstrongylus megistus

Isolation and molecular characterization of a major hemolymph serpin from the triatomine, Panstrongylus megistus

Characterization of Guinea Pig Antibody Responses to Salivary Proteins of Triatoma infestans for the Development of a Triatomine Exposure Marker

Caracterização de resistência a inseticidas em populações da subfamilia Triatominae (Hemiptera:Reduviidae), vetores de Trypanosoma cruzi Chagas, 1909

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