Variability of virulence of Nectria galligena towards northern hardwoods

Plante, F.; Bernier, Louis

doi:10.1111/j.1439-0329.1997.tb00868.x

Cited by 11 publications

(4 citation statements)

References 17 publications

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“…All pairings of either MAT1-1-1 or MAT1-2-1 strains from different plant hosts resulted in perithecia formation. While a lack of host specificity by N. ditissima has been previously demonstrated using pathogenicity assays (Lortie 1969;Ng and Roberts 1974;Barnard et al 1988;Plante and Bernier 1997), mating among N. ditissima strains infecting co-occurring tree species had not been previously tested until now. Given the lack of reproductive barriers, evolution of host specificity is limited by obligate outcrossing among these co-occurring strains.…”

Section: Discussionmentioning

confidence: 99%

Characterization of mating type genes in heterothallicNeonectriaspecies, with emphasis onN. coccinea, N. ditissima, andN. faginata

et al. 2020

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Neonectria ditissima and N. faginata are canker pathogens involved in an insect-fungus disease complex of American beech (Fagus grandifolia) commonly known as beech bark disease (BBD). In Europe, both N. ditissima and N. coccinea are involved in BBD on European beech (Fagus sylvatica). Field observations across the range of BBD indicate that new infections occur primarily via ascospores. Both heterothallic (selfsterile) and homothallic (self-fertile) mating strategies have been reported for Neonectria fungi. As such, investigations into mating strategy are important for understanding both the disease cycle and population genetics of Neonectria. This is particularly important in the U.S. given that over time N. faginata dominates the BBD pathosystem despite high densities of non-beech hosts for N. ditissima. This study utilized whole-genome sequences of BBD-associated Neonectria spp. along with other publicly available Neonectria and Corinectria genomes and in vitro mating assays to characterize mating type (MAT) loci and confirm thallism for select members of Neonectria and Corinectria.MAT gene-specific primer pairs were developed to efficiently characterize the mating types of additional single ascospore strains of N. ditissima, N. faginata, and N. coccinea and several other related species lacking genomic data. In vitro mating assays were used in combination with molecular results to confirm thallism. These assays also comfirmed the sexual compatibility among N. ditissima strains from different plant hosts.Maximum likelihood phylogenetic analysis of both MAT1-1-1 and MAT1-2-1 sequences recovered trees with similar topology to previously published phylogenies of Neonectria and Corinectria. The results of this study indicate that all Neonectria and Corinectria

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Section: Discussionmentioning

confidence: 99%

Characterization of mating type genes in heterothallicNeonectriaspecies, with emphasis onN. coccinea, N. ditissima, andN. faginata

et al. 2020

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“…In elms, dieback symptoms have been associated with colonization by Nectria sp. (Heybroek, 1993;Plante and Bernier, 1997).…”

Section: Phenotypic Vitality and Wood Mycobiomementioning

confidence: 99%

Core endophytic mycobiome in Ulmus minor and its relation to Dutch elm disease resistance

Macaya‐Sanz

Witzell²,

Collada³

et al. 2023

Front. Plant Sci.

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The core microbiota of plants exerts key effects on plant performance and resilience to stress. The aim of this study was to identify the core endophytic mycobiome in U. minor stems and disentangle associations between its composition and the resistance to Dutch elm disease (DED). We also defined its spatial variation within the tree and among distant tree populations. Stem samples were taken i) from different heights of the crown of a 168-year-old elm tree, ii) from adult elm trees growing in a common garden and representing a gradient of resistance to DED, and iii) from trees growing in two distant natural populations, one of them with varying degrees of vitality. Endophyte composition was profiled by high throughput sequencing of the first internal transcribed spacer region (ITS1) of the ribosomal DNA. Three families of yeasts (Buckleyzymaceae, Trichomeriaceae and Bulleraceae) were associated to DED-resistant hosts. A small proportion (10%) of endophytic OTUs was almost ubiquitous throughout the crown while tree colonization by most fungal taxa followed stochastic patterns. A clear distinction in endophyte composition was found between geographical locations. By combining all surveys, we found evidence of a U. minor core mycobiome, pervasive within the tree and ubiquitous across locations, genotypes and health status.

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“…An extensive dieback caused by the fungus Chalarea fraxinea has been observed in Europe since the 1990s (KRAJ et al, 2012). Differences in timing and severity of the disease among different parts of the range may be associated with the dynamics of the spread, but also with differential, genetically determined susceptibility of local populations to the infection, associated with geographic origin (PLANTE and BERNIER, 1997).…”

Section: Introductionmentioning

confidence: 99%

Gene exchange across a postglacial contact zone in Fraxinus excelsior L.

et al. 2012

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Hybridization between divergent lineages of common ash Fraxinus excelsior L. was studied in Slovakia and adjacent regions of Hungary, the Czech Republic and Poland in the contact zone between two postglacial colonization routes originating from different glacial refugia. Thirty-eight common ash populations were studied using a combination of 6 maternally inherited chloroplast microsatellite markers and 7 biparentally inherited nuclear microsatellites (nSSR). Two predominant chloroplast lineages were identified, between which the boundary was very sharp. Populations containing a mixture of different haplotypes were found only in the immediate proximity of the boundary. Bayesian analysis of population structure based on nSSR loci revealed the existence of two clusters, which largely coincided with chloroplast lineages. Both haplotype frequencies and proportions of clusters identified by the Bayesian analysis exhibited a clinal transition over the hybrid zone, with cline widths of 36 km for chloroplast haplotype frequencies (reflecting gene flow by seeds) and 275 km for Bayesian clusters based on nSSR (reflecting gene flow by pollen and seeds). Chloroplast haplotype diversity increased along the boundary between lineages not only because of admixture, but also due to the presence of rare haplotypes. In contrast, diversity at nuclear loci did not exhibit any geographical trend.

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Variability of virulence of Nectria galligena towards northern hardwoods

Cited by 11 publications

References 17 publications

Characterization of mating type genes in heterothallicNeonectriaspecies, with emphasis onN. coccinea, N. ditissima, andN. faginata

Characterization of mating type genes in heterothallicNeonectriaspecies, with emphasis onN. coccinea, N. ditissima, andN. faginata

Core endophytic mycobiome in Ulmus minor and its relation to Dutch elm disease resistance

Gene exchange across a postglacial contact zone in Fraxinus excelsior L.

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