Variable amounts of translatable ferritin mRNA in bean leaves with various iron contents

Mark, F. van der; Bienfait, Frits; Ende, H. van den

doi:10.1016/s0006-291x(83)80167-3

Cited by 33 publications

(14 citation statements)

References 8 publications

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“…Such is not the case: no ferritin mRNA was detected by hybridization (Fig. 2) or translation (30) in iron-depleted cultured soybean cells; similar results were obtained (29) when translatable ferritin mRNA in leaves from iron-deficient and iron-loaded bean plants was measured.…”

Section: Discussionsupporting

confidence: 71%

“…Iron increased the amount of translatable ferritin mRNA in bean leaves and in soybean cell cultures by =40-fold (29,30). To quantify the effect of iron on induction of ferritin mRNA and protein, total cellular RNAs of irontreated soybean cells were analyzed by electrophoresis and by hybridization.…”

Section: Resultsmentioning

confidence: 99%

“…Induction of transcription of ferritin mRNA by iron in animal cells is accompanied by changes in the protein structure (25)(26)(27)(28)(29) and, often, in the iron content (25)(26)(27). In contrast, in the plant cell both the ferritin mRNA and protein induced in iron-treated soybean cells appear to be unchanged from the normal or constitutive type (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Earlier measurements of translatable ferritin mRNAs suggested that in contrast to animals, iron regulation may affect mRNA transcription rather than translation in bean leaves (29) and cultured soybean cells (30). Measurement of ferritin mRNA by translation in cellfree extracts could have been complicated by ferritin-mRNAspecific translational effects (13).…”

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confidence: 99%

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Ferritin gene transcription is regulated by iron in soybean cell cultures.

Lescure

Proudhon

Pesey

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

171

164

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Section: Discussionsupporting

confidence: 71%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Ferritin gene transcription is regulated by iron in soybean cell cultures.

Lescure

Proudhon

Pesey

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

171

164

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“…Copper may have controlled DAO biosynthesis by modulating the level of mRNA coding for DAO as occurs for some metalbinding proteins where the metal regulates the amount of protein synthesized (3,19). However, to date, there are no reports of a metalloenzyme being controlled by its metal cofactor at the level of transcription.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Copper Nutrition and Developmental State on the Biosynthesis of Diamine Oxidase in Clover Leaves

Delhaize¹,

Dilworth²,

Webb³

1986

Plant Physiol.

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The biosynthesis of diamine oxidase (DAO; EC 1.4.3.6) in leaf blades of subterranean clover (Trifolium subterraneum L. cv Seaton Park) was followed by labeling whole plants with "4CO2. A pulse-chase experiment where DAO was immunoprecipitated with anti-DAO antibodies showed that only leaf primordia and the youngest emerged leaves were able to synthesize the enzyme. The amount of DAO in young leaves of clover grown with a range of Cu treatments was determined by its enzymic activity and by single radial immunodiffusion against anti-DAO antibodies; both parameters were highly correlated with the Cu concentration of the leaf. Further, anti-DAO antibodies reacted against apo-DAO prepared in vitro indicating that apo-DAO was absent from Cu-deficient leaves. These results suggest that the biosynthesis of DAO in young clover leaves is controlled by the Cu concentrations of the leaves. Poly(A) mRNA purified from leaf primordia and young emerging leaves of plants with either a high Cu or low Cu supply was translated in wheat germ and rabbit reticulocyte cell-free systems. No differences between the two Cu treatments could be seen in the sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles of the translation products after fluorography. However, anti-DAO antibodies did not detect any DAO synthesized in vitro from either treatment. available.In this paper we confirm, with the use ofantibodies, that DAO is synthesized only in young developing leaves and that apo-DAO is absent from Cu-deficient clover leaves. MATERIALS AND METHODSPlant Material. Subterranean clover (Trifolium subterraneum L. cv Seaton Park) was grown on a Cu-deficient sand that had been treated and potted in 3 kg portions as previously described (13). Fifteen pregerminated clover seeds were sown in each pot and after 13 d the plants were thinned to 6/pot. The basal nutrient contained 280 mg NH4NO3 to avoid problems of Ndeficiency arising from effects of Cu-deficiency on N-fixation in nodules. To study the effect ofCu nutrition on DAO biosynthesis in leaves, plants were grown with the following rates of Cu as CuSO4. 5H20; 0, 200, 400, 800, and 1600 ug/3 kg pot.The nomenclature used to describe the stages of leaf development was that of Delhaize et al. (6) and is as follows:Youngest emerged leaf: the unopened terminal leaf on the stem. These leaves were either enclosed within subtending stipules or supported by short stipules. Leaf size was in the range of 4 to 7 mm. Young folded leaf: the youngest leaf where the leaflets are still opening. Petiole length usually less than 2 cm.Youngest open leaf: the youngest leaf on the main stem where the leaflets have completely opened but have not attained maximum size.Youngest mature leaf: the youngest fully expanded leaf. Leaves younger than the youngest emerged leaf were collectively termed leaf primordia.DAO Assay. DAO activity in leaf homogenates was assayed with an 02 electrode by measuring the rate that 02 was consumed when putrescine was used as a substrate under the conditions described by L...

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Abscisic acid is involved in the iron-induced synthesis of maize ferritin.

1993

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The ubiquitous iron storage protein ferritin has a highly conserved structure in plants and animals, but a distinct cytological location and a different level of control in response to iron excess. Plant ferritins are plastid‐localized and transcriptionally regulated in response to iron, while animal ferritins are found in the cytoplasm and have their expression mainly controlled at the translational level. In order to understand the basis of these differences, we developed hydroponic cultures of maize plantlets which allowed an increase in the intracellular iron concentration, leading to a transient accumulation of ferritin mRNA and protein (Lobréaux,S., Massenet,O. and Briat,J.F., 1992, Plant Mol. Biol., 19, 563–575). Here, it is shown that iron induces ferritin and RAB (Responsive to Abscisic Acid) mRNA accumulation relatively with abscisic acid (ABA) accumulation. Ferritin mRNA also accumulates in response to exogenous ABA. Synergistic experiments demonstrate that the ABA and iron responses are linked, although full expression of the ferritin genes cannot be entirely explained by an increase in ABA concentration. Inducibility of ferritin mRNA accumulation by iron is dramatically decreased in the maize ABA‐deficient mutant vp2 and can be rescued by addition of exogenous ABA, confirming the involvement of ABA in the iron response in plants. Therefore, it is concluded that a major part of the iron‐induced biosynthesis of ferritin is achieved through a pathway involving an increase in the level of the plant hormone ABA. The general conclusion of this work is that the synthesis of the same protein in response to the same environmental signal can be controlled by separate and distinct mechanisms in plants and animals.

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Variable amounts of translatable ferritin mRNA in bean leaves with various iron contents

Cited by 33 publications

References 8 publications

Ferritin gene transcription is regulated by iron in soybean cell cultures.

Ferritin gene transcription is regulated by iron in soybean cell cultures.

The Effects of Copper Nutrition and Developmental State on the Biosynthesis of Diamine Oxidase in Clover Leaves

Abscisic acid is involved in the iron-induced synthesis of maize ferritin.

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