Variable Ca sensitivity of a K-selective channel in intact red-cell membranes

Lew, Virgilio L.; Ferreira, H. G.

doi:10.1038/263336a0

Cited by 136 publications

(60 citation statements)

References 18 publications

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“…Additionally, when treated with Ca 2+ and A23187, human erythrocytes and lymphocytes respond by budding to release membrane vesicles (Allan and Michell, 1975;Allan et al, 1976a;PodszywalowBartnicka et al, 2007). This treatment also induces several other biochemical changes, including K + efflux (Lew and Ferreira, 1976;Dupuis et al, 1989), the breakdown of polyphosphoinositides, a rise in 1,2-diacylglycerol and phosphatidate concentrations (Allan et al, 1976b;Allan and Michell, 1978;Ponnappa et al, 1980;Allan and Thomas, 1981;Butikofer et al, 1993), and a selective loss of glycosylphosphatidylinositol-anchored membrane proteins (Butikofer et al, 1993). The treatment of hematopoietic cells with A23187 can thus be regarded as a model with which to investigate the biochemical mechanisms responsible for membrane composition during intracellular calcium elevation.…”

Section: Discussionmentioning

confidence: 99%

Macrophage Recognition of Cells with Elevated Calcium Is Mediated by Carbohydrate Chains of CD43

Miki

Oguri

Hirano

et al. 2013

Cell Struct. Funct.

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ABSTRACT. Macrophages remove deteriorating cells (those undergoing apoptosis and oxidation) via poly-Nacetyllactosaminyl chains on CD43 caps, a major cell-surface glycoprotein. Unusually high intracellular calcium levels are also deteriorating for cells and tissue. Here we artificially elevated calcium levels in cells and examined the mechanism by which this elevation was resolved by macrophages. Results showed that treatment with the calcium ionophore A23187 and ionomycin induces capping of CD43 on Jurkat cells, which are subsequently recognized and phagocytosed by macrophages, indicating that macrophages regard cells with elevated calcium as targets for removal. Further tests showed that A23187-and ionomycin-treated Jurkat cells did not induce apoptotic changes such as DNA fragmentation or phosphatidylserine expression, indicating that these cells were removed despite still being viable. Jurkat cells pretreated with anti-CD43 antibody or those with poly-Nacetyllactosaminyl chains containing oligosaccharides inhibited macrophage binding, indicating that macrophages recognize the poly-N-acetyllactosaminyl chains on CD43. Binding was also inhibited by treating macrophages with anti-nucleolin antibody, indicating that recognition occurs through nucleolin, a cell-surface receptor. Further, nucleolin-transfected HEK293 cells bound A23187-treated cells, and this binding was inhibited by in the presence of oligosaccharides. Taken together, these results show that elevated calcium levels induce CD43 capping, and macrophages remove the cells if their nucleolin receptors can bind to the poly-Nacetyllactosaminyl chains of capped CD43.

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Section: Discussionmentioning

confidence: 99%

Macrophage Recognition of Cells with Elevated Calcium Is Mediated by Carbohydrate Chains of CD43

Miki

Oguri

Hirano

et al. 2013

Cell Struct. Funct.

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“…In some experiments cytoplasmic Ca2+ was controlled by a modification of the method used on human erythrocytes by Lew & Ferreira (1976). Rodent red cells were obtained and prepared as described previously, then washed twice in flux medium of the composition (mM): KCI, 75; NaCl, 75; glucose or inosine, 10; MgC12, 0 5; CaCI2, 0 5; MOPS,15 (pH 7 9 at room temperature).…”

Section: Temperature On K+ Permeabilitymentioning

confidence: 99%

“…The slope of the relationship -In (1 -At/A ) vs. time was then computed by least-squares linear regression analysis, giving the rate constant for 42K equilibration (see Lew & Ferreira, 1976 exhibited the classically biphasic dependence originally described for human red cells (Glynn, 1956) at both 37 and 5 'C in both species (Figs. 1 and 2).…”

Section: Temperature On K+ Permeabilitymentioning

confidence: 99%

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Differential effects of temperature on three components of passive permeability to potassium in rodent red cells.

Hall¹,

Willis²

1984

The Journal of Physiology

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SUMMARYThe effect of temperature on ouabain-insensitive fluxes of K+ was characterized in red cells from a non-hibernator (guinea-pig) and a hibernator (thirteen-lined ground squirrel).1. The residual K+ influx which remains in the presence ofouabain and bumetanide, and which is linearly dependent on [K+]. was the same in the erythrocytes of the two species at low temperature (5 TC). 2. At 5 'C co-transport of K+ was abolished in guinea-pig red cells but was still present in ground squirrel red cells.3. In guinea-pig cells, ouabain-and-bumetanide-insensitive K+ flux was increased by Ca2+ at low temperatures. This flux was inhibited by quinine and selective for K+ over Na+, indicating activation of the Ca2+-sensitive K+ pathway (Gardos channel).4. Ouabain-and-bumetanide-insensitive K+ permeability in red cells from the gound squirrel was insensitive to Ca2+ added to the medium at low temperature. When ground squirrel red cells were depleted of ATP or treated with A23187, Ca2+ induced a flux which was inhabitable by quinine. Hence, ground squirrel red cells possess Gairdos channels.5. The temperature sensitivity of the K+ channels was assessed using A23187-mediated K+ influx as a measure of Gardos channel activation. The influence of temperature on the Ca2+-stimulated K+ fluxes under these conditions was indistinguishable between the two species.6. It is concluded that K+ loss through the Ca2+-sensitive K+ channel is minimal in hibernators' erythrocytes because of more efficient regulation of cytoplasmic Ca2+ during cold storage.

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“…A number of agents such as Pb2' [ 1,2], propranolol ] 3,4] or the divaient cation ionophore A23187 + Ca2* [5,6] produce in the human erythrocyte a loss of K* by activation of the so-called Ca2'-dependent K' channel. The same effects were obtained treating the cells with the electron donor system ascorbate t phenazine methosulphate (asc-PMS), the K fluxes being preventedin this case by several presumed oxidoreductase inhibitors (chlorproma~ne, atebrin, ~t~mycin A).…”

Section: Introductionmentioning

confidence: 99%

Effects of several inhibitors on the K⁺ efflux induced by activation of the Ca²⁺‐dependent channel and by valinomycin in the human red cell

1980

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Variable Ca sensitivity of a K-selective channel in intact red-cell membranes

Cited by 136 publications

References 18 publications

Macrophage Recognition of Cells with Elevated Calcium Is Mediated by Carbohydrate Chains of CD43

Macrophage Recognition of Cells with Elevated Calcium Is Mediated by Carbohydrate Chains of CD43

Differential effects of temperature on three components of passive permeability to potassium in rodent red cells.

Effects of several inhibitors on the K⁺ efflux induced by activation of the Ca²⁺‐dependent channel and by valinomycin in the human red cell

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Variable Ca sensitivity of a K-selective channel in intact red-cell membranes

Cited by 136 publications

References 18 publications

Macrophage Recognition of Cells with Elevated Calcium Is Mediated by Carbohydrate Chains of CD43

Macrophage Recognition of Cells with Elevated Calcium Is Mediated by Carbohydrate Chains of CD43

Differential effects of temperature on three components of passive permeability to potassium in rodent red cells.

Effects of several inhibitors on the K+ efflux induced by activation of the Ca2+‐dependent channel and by valinomycin in the human red cell

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Effects of several inhibitors on the K⁺ efflux induced by activation of the Ca²⁺‐dependent channel and by valinomycin in the human red cell