Variable effects of oxytetracycline on antibiotic resistance gene abundance and the bacterial community during aerobic composting of cow manure

Qian, Xiaoqing; Sun, Wei; Gu, Jie; Wang, Xiaojuan; Sun, Jiajun; Yin, Yanan; Duan, Manli

doi:10.1016/j.jhazmat.2016.05.002

Cited by 238 publications

(68 citation statements)

References 52 publications

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“…According to PCoA, PC1, and PC2 accounted for 70.49% of the total variation in the bacterial community (Supplementary Figure S4). The bacterial community structure was separated significantly during different composting periods, thereby indicating that the composting period played an important role in shaping the microbial community, as also shown in previous studies (Qian et al, 2016a; Zhang et al, 2016b). However, Cu2000 was most distinct from the control beyond day 2, and it tended toward the days 0–2 clusters (Supplementary Figure S4).…”

Section: Resultssupporting

confidence: 83%

“…Thus, the abundance of intI1 decreased by 0.7–1.1 logs during the thermophilic phase, but its abundance was stable throughout the subsequent composting period. Cu2000 significantly improved the abundance of intI1 , and a high absolute abundance of intI1 in compost can enhance the dissemination of ARGs in the soil environment (Su et al, 2015; Qian et al, 2016a). …”

Section: Resultsmentioning

confidence: 99%

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Effects of Copper Addition on Copper Resistance, Antibiotic Resistance Genes, and intl1 during Swine Manure Composting

et al. 2017

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Copper is one of the most abundant heavy metals present in swine manure. In this study, a laboratory-scale aerobic composting system was amended with Cu at three levels (0, 200, and 2000 mg kg-1, i.e., control, Cu200, and Cu2000 treatments, respectively) to determine its effect on the fate of copper resistance genes [copper resistance genes (CRGs): pcoA, cusA, copA, and tcrB], antibiotic resistance genes [antibiotic resistance genes (ARGs): erm(A) and erm(B)], and intl1. The results showed that the absolute abundances of pcoA, tcrB, erm(A), erm(B), and intl1 were reduced, whereas those of copA and cusA increased after swine manure composting. Redundancy analysis showed that temperature significantly affected the variations in CRGs, ARGs, and intl1. The decreases in CRGs, ARGs, and intI1 were positively correlated with the exchangeable Cu levels. The bacterial community could be grouped according to the composting time under different treatments, where the high concentration of copper had a more persistent effect on the bacterial community. Network analysis determined that the co-occurrence of CRGs, ARGs, and intI1, and the bacterial community were the main contributors to the changes in CRGs, ARG, and intl1. Thus, temperature, copper, and changes in the bacterial community composition had important effects on the variations in CRGs, ARGs, and intl1 during manure composting in the presence of added copper.

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Effects of Copper Addition on Copper Resistance, Antibiotic Resistance Genes, and intl1 during Swine Manure Composting

et al. 2017

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“…The attenuation efficiency of target genes by non‐sterile and sterile larvae treatments was higher than that by the control treatment. Their average reduction rates and treatment times were, respectively, higher and lower than those in previous studies, such as in the polymers‐adding swine manure composting (8.1%–96.7% decrease of 35 days) (Guo et al ., ), in cow manure composting (below 35.1% reduction of 40 days) (Qian et al ., ), in commercial poultry manure composting (average 37.1% decline of 30 days) (Xie et al ., ) and in hyperthermophilic composting of sewage sludge (average 89% removal of 45 days) (Liao et al ., ). For the attenuated tet genes, we used the sum of their attenuated values in a sample to approximate the total attenuation of the treatment process (Fig.…”

Section: Resultsmentioning

confidence: 99%

Rapidly mitigating antibiotic resistant risks in chicken manure by Hermetia illucens bioconversion with intestinal microflora

Cai

et al. 2018

Environmental Microbiology

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Summary Antibiotic resistance genes (ARGs) in animal manure are an environmental concern due to naturally occurring bacteria being exposed to these wastes and developing multidrug resistance. The bioconversion of manure with fly larvae is a promising alternative for recycling these wastes while attenuating ARGs. We investigated the impact of black soldier fly (BSF, Hermetia illucens) larval bioconversion of chicken manure on the persistence of associated ARGs. Compared with traditional composting or sterile larval treatments (by 48.4% or 88.7%), non‐sterile BSF larval treatments effectively reduced ARGs and integrin genes by 95.0% during 12 days, due to rapid decreases in concentrations of the genes and associated bacteria as they passed through the larval gut and were affected by intestinal microbes. After larval treatments, bacterial community composition differed significantly, with the percentage of Firmicutes possibly carrying ARGs reduced by 65.5% or more. On average, human pathogenic bacteria populations declined by 70.7%–92.9%, effectively mitigating risks of these bacteria carrying ARGs. Environmental pH, nitrogen content and antibiotic concentrations were closely related to both bacterial community composition and targeted gene attenuation in larval systems. Selective pressures of larval gut environments with intestinal microbes, larval bacteriostasis and reformulation of manure due to larval digestion contributed to ARG attenuation.

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“…Many studies have examined the effects of a variety of technologies on the reduction of antibiotics, ARB and ARGs, including but not limited to anaerobic digestion, composting, and activated sludge (Feng, Casas, Ottosen, Moller, & Bester, ; Qian, Sun, Gu, Wang, Zhang, et al., ; Shan, Wang, Fang, Chu, & Jiang, ; Zhang et al., , ). Nevertheless, studies quantifying the effects of these processes on the control of antibiotic resistance are often conflicting, as variables including animal age, livestock waste properties, and operational conditions are different (Qian, Sun, Gu, Wang, Sun, et al., ; Selvam, Xu, Zhao, & Wong, ). Additionally, though various single processes have been investigated, the integrated treatment system is rarely assessed for effectiveness on antibiotic resistance control.…”

Section: Introductionmentioning

confidence: 99%

Fates of antibiotic resistance genes in a distributed swine wastewater treatment plant

Yuan

Zhai

Mao

et al. 2019

Water Environment Research

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This study explores the prevalence, emission, and reduction of five ARGs (sulI, tetA, mphB, qnrD, and mcr‐1) and integron (intI) through a distributed swine wastewater purification facility and the effluent‐receiving environment. Typical metal resistance genes (MRGs), pathogenic bacterial indicators, the bacterial community, and wastewater properties were also explored to determine their effects on the fates of ARGs. Results indicated that the purification process could hardly effectively remove ARGs’ prevalence. 3.1 × 104–7.1 × 108 copies/L were present after purification, and 4%–57% of them persisted in the subsequent creek and adjacent soil. 16S rRNA sequencing suggested that the discharge of wastewater significantly changed the bacterial community in receiving creek and soil. Molecular ecological networks analysis detected the wide co‐occurrence among ARGs, MRGs, and PBGs, which could further facilitate the propagation of antibiotic resistance. ARG incidence and specific bacterial genera were closely correlated, suggesting an extensive hosting relationship. Redundancy analyses showed wastewater organics and nutrients showed positive correlation to most ARGs’ abundance, but negatively correlated to their relative abundance. Practitioner points Fate of five ARGs and intI was studied in a swine wastewater treatment system. The treatment process could not effectively reduce ARGs’ abundance. ARGs and pathogens in wastewater were transferred to the receiving creek and soil. The network analysis found wide co‐occurrence among ARGs, metal resistance genes, and pathogens. Wastewater nutrients positively correlated to ARG's abundance but negatively correlated to their relative abundance.

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Variable effects of oxytetracycline on antibiotic resistance gene abundance and the bacterial community during aerobic composting of cow manure

Cited by 238 publications

References 52 publications

Effects of Copper Addition on Copper Resistance, Antibiotic Resistance Genes, and intl1 during Swine Manure Composting

Effects of Copper Addition on Copper Resistance, Antibiotic Resistance Genes, and intl1 during Swine Manure Composting

Rapidly mitigating antibiotic resistant risks in chicken manure by Hermetia illucens bioconversion with intestinal microflora

Fates of antibiotic resistance genes in a distributed swine wastewater treatment plant

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