Variable Levels of Long Noncoding RNA Expression in DNA Mismatch Repair-Proficient Early-Stage Colon Cancer

Li, Qian; Li, Nanshan; Lao, Yueqiong; Wu, Lin; Jiang, Guojun; Wu, Nan; Wang, Canghai; Liu, Kuiliang; Wu, Jing

doi:10.1007/s10620-017-4465-6

Cited by 9 publications

(8 citation statements)

References 28 publications

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“…In this issue of Digestive Diseases and Sciences , Li et al [7] report their experience with lncRNAs in colorectal cancer (CRC). They extracted total RNA from 4 early-stage CRCs, profiling the RNA using a commercially available microarray that included 77,103 lncRNAs and 18,853 mRNAs.…”

mentioning

confidence: 99%

“…Liu et al [8] have reported that the exosomal lncRNA CRNDE-h is elevated in the serum of patients with CRC, which correlated with the degree of metastasis. [7] Sun and colleagues mined the Gene Expression Omnibus public database that used lncRNA microarrays on cases of CRC, identifying 15 dysregulated lncRNAs, which they validated using quantitative PCR on 84 of CRC samples. [8] Wang et al reported that the lncRNA NNT-AS1 is upregulated in CRC tissues, its abundance correlated with metastasis, and its inhibition in laboratory models decreased the malignant behavior of the cells.…”

mentioning

confidence: 99%

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Non-coding RNA: It’s Not Junk

Boland

2017

Dig Dis Sci

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confidence: 99%

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confidence: 99%

Non-coding RNA: It’s Not Junk

Boland

2017

Dig Dis Sci

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“…As in our previous study [18] , multiplex PCR and immunohistochemistry were used to determine the MMR status. pMMR colonic tissues were de ned as tissue samples with a low frequency of MSI (MSI-L) or microsatellite stability (MSS, no marker tested).…”

Section: Screening Of Pmmr Samples and Total Rna Isolationmentioning

confidence: 99%

Comprehensive Analysis of Differentially Expressed Long Non-Coding RNA-mRNA in The Adenoma–Carcinoma Sequence of DNA Mismatch Repair Proficient Colon Cancer

et al. 2020

Preprint

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Abstract Background DNA mismatch repair proficient colon cancer (pMMR CC) is the most common subtype of sporadic CC. However, the role of long non-coding RNAs (lncRNAs) in pMMR CC carcinogenesis has not been fully elucidated. Methods In the present study, we conducted transcriptomic analysis of lncRNAs-mRNAs in five low-grade intraepithelial neoplasia (LGIN), five high-grade intraepithelial neoplasia (HGIN), four pMMR CC, and five normal control (NC) tissues. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment pathway, and Co-Expression Network analyses were performed to elucidate the functions of lncRNAs and mRNAs as well as their interactions. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate five dysregulated lncRNAs in a large set of colon tissues. Receiver-operating characteristic (ROC) curves were employed to evaluate the performance of the candidate lncRNAs. Results A set of 5783 differentially expressed lncRNAs and 4483 differentially expressed mRNAs were detected among the LGIN, HGIN, pMMR CC, and NC samples. These differentially expressed lncRNAs and mRNAs were assigned to 275 significant GO terms and 179 significant KEGG enriched pathways. qRT-PCR confirmed that the expression of five selected lncRNAs (ENST00000521815, ENST00000603052, ENST00000609220, NR_026543, and ENST00000545920) were consistent with the microarray data. ROC analysis showed that four lncRNAs (ENST00000521815, ENST00000603052, ENST00000609220, and NR_026543) had larger area under the ROC curve (AUC) values than serum carcinoembryonic antigens, thereby distinguishing NC from pMMR CC. Conclusions Several lncRNAs play various roles in the adenoma–carcinoma sequence and may serve as potential biomarkers for the early diagnosis of pMMR CC.

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“…Through analyzing lncRNAs and its regulated mRNA in colon cancer tissues, the data showed that a interaction with lncRNAs and mRNAs was involved in cellular activity of cell division, angiogenesis, apoptotic, et al [59]. LncRNAs maternally expressed gene 3 (MEG3) was located on human chromosome 14.…”

Section: The Role Of Lncrnas In Crcmentioning

confidence: 99%

The Biological Effect and Clinical Application of Long Noncoding RNAs in Colorectal Cancer

Sun¹,

Wang²,

Chen³

et al. 2018

Cell Physiol Biochem

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Colorectal cancer (CRC) is one of the most common malignancies in the world. Easier recurrence and metastasis is the main cause of mortality in CRC patients, and the markers applied for diagnosis and treatment of CRC is still urgently needed to early diagnose and evaluate therapeutic effect. Long noncoding RNA (lncRNA) is a class of noncoding RNA that the length is more than 200 nucleotides. With the development of sequencing technique about transcriptome, increasing lncRNAs are focused on their function and mechanism related to the nosogenesis and pathology of CRC. Recent studies report that lncRNAs acted as crucial role in CRC and could be as biomarker for CRC diagnosis and treatment. In this review, we display the regulation of lncRNA by interacting with DNA, RNA and protein and highlight the double role of lncRNAs as oncogene or anti-tumor gene involved in Wnt signaling pathway, p53 signaling pathway or others to be an regulator in CRC development. Lastly, we discuss some new finding of lncRNAs, especially lncRNA in exosome, which could be as potential markers for diagnosis and treatment of CRC in future.

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Variable Levels of Long Noncoding RNA Expression in DNA Mismatch Repair-Proficient Early-Stage Colon Cancer

Abstract: Differentially expressed lncRNAs were significantly associated with clinical features of patients with pMMR CC and may participate in the tumorigenesis of pMMR CC.

Cited by 9 publications

References 28 publications

Non-coding RNA: It’s Not Junk

Non-coding RNA: It’s Not Junk

Comprehensive Analysis of Differentially Expressed Long Non-Coding RNA-mRNA in The Adenoma–Carcinoma Sequence of DNA Mismatch Repair Proficient Colon Cancer

The Biological Effect and Clinical Application of Long Noncoding RNAs in Colorectal Cancer

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