Variable lipoprotein haemagglutinin (vlhA) gene sequence typing of mainly DutchMycoplasma synoviaeisolates: comparison withvlhAsequences from Genbank and with amplified fragment length polymorphism analysis

Dijkman, Remco; Feberwee, Anneke; Landman, W. J. M.

doi:10.1080/03079457.2014.958980

Cited by 13 publications

(13 citation statements)

References 43 publications

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“…Similarly, 15 samples originating from the oviduct of hens producing eggs with EAA represented nine STs belonging to at least three different CCs (ST12 Cplx, ST26 Cplx and ST56 Cplx). This is in agreement with earlier research showing that isolates with distinct vlhA ST can be obtained from the oviduct of hens producing EAA (Dijkman et al, 2014). Further research is needed to identify the genetic factors of M. synoviae that may explain its variations in tissue tropism and disease inducing potential.…”

Section: Discussionsupporting

confidence: 90%

“…This study showed that MLST based on five genes has a higher discriminatory power than vlhA sequence typing. Moreover, recent studies have shown that vlhA sequence typing can be used successfully for the differentiation between MS-H and M. synoviae field strains in specific geographical areas (Harada et al, 2009;Bayatzadeh et al, 2014), but is not always discriminatory enough (Jeffery et al, 2007;Ogino et al, 2011;Dijkman et al, 2014). Recently, it was shown that the MS-H vaccine strain could be distinguished from all M. synoviae field strains (Shahid et al, 2014) by high resolution melting curve analysis and sequencing of the obg gene making this gene a better target for vaccine strain differentiation.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years sequencing of the variable lipoprotein and haemagglutinin (vlh) A gene (Benčina et al, 2001;Hong et al, 2004;Jeffery et al, 2007;Hammond et al, 2009;Harada et al, 2009;Wetzel et al, 2010;El-Gazzar et al, 2012;Dijkman et al, 2014) or restriction fragment length polymorphism of the vlhA gene (Bayatzadeh et al, 2014) had replaced several other molecular typing techniques, like restriction endonuclease analysis of DNA (Morrow et al, 1990b;Ley & Avakian, 1992), pulsed-field gel electrophoresis (Marois et al, 2001;Dufour-Gesbert et al, 2006;Harada et al, 2009), amplified fragment length polymorphism (Feberwee et al, 2005), analysis of the 16S to 23S rRNA intergenic spacer region of M. synoviae (Ramirez et al, 2006(Ramirez et al, , 2011 and random amplified polymorphic DNA analysis (Geary et al, 1994;Marois et al, 2001;Feberwee et al, 2005;Dufour-Gesbert et al, 2006) for typing M. synoviae isolates as these were too labour intensive and therefore costly for large scale application.…”

Section: Introductionmentioning

confidence: 99%

“…The discriminatory power of vlhA sequence typing for molecular epidemiology of at least Dutch M. synoviae isolates proved to be insufficient and a typing technique based on more genes was expected to improve discriminatory power (Dijkman et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Development and evaluation of a multi-locus sequence typing scheme for Mycoplasma synoviae

2016

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Reproducible molecular Mycoplasma synoviae typing techniques with sufficient discriminatory power may help to expand knowledge on its epidemiology and contribute to the improvement of control and eradication programmes of this mycoplasma species. The present study describes the development and validation of a novel multi-locus sequence typing (MLST) scheme for M. synoviae. Thirteen M. synoviae isolates originating from different poultry categories, farms and lesions, were subjected to whole genome sequencing. Their sequences were compared to that of M. synoviae reference strain MS53. A high number of single nucleotide polymorphisms (SNPs) indicating considerable genetic diversity were identified. SNPs were present in over 40 putative target genes for MLST of which five target genes were selected (nanA, uvrA, lepA, ruvB and ugpA) for the MLST scheme. This scheme was evaluated analysing 209 M. synoviae samples from different countries, categories of poultry, farms and lesions. Eleven clonal clusters and 76 different sequence types (STs) were obtained. Clustering occurred following geographical origin, supporting the hypothesis of regional population evolution. M. synoviae samples obtained from epidemiologically linked outbreaks often harboured the same ST. In contrast, multiple M. synoviae lineages were found in samples originating from swollen joints or oviducts from hens that produce eggs with eggshell apex abnormalities indicating that further research is needed to identify the genetic factors of M. synoviae that may explain its variations in tissue tropism and disease inducing potential. Furthermore, MLST proved to have a higher discriminatory power compared to variable lipoprotein and haemagglutinin A typing, which generated 50 different genotypes on the same database.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development and evaluation of a multi-locus sequence typing scheme for Mycoplasma synoviae

2016

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“…Genotyping based on the vlhA gene is used worldwide for the molecular differentiation of M . synoviae strains, but this method was proved to be unreliable in the discrimination of the MS-H vaccine strain [11,18–19]. High resolution melt-curve analysis based assay was developed to identify specific SNPs in the obg gene in order to distinguish the ts + MS-H vaccine strain from its ts - re-isolates and field strains [11].…”

Section: Discussionmentioning

confidence: 99%

Development of mismatch amplification mutation assays for the differentiation of MS1 vaccine strain from wild-type Mycoplasma synoviae and MS-H vaccine strains

et al. 2017

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Mycoplasma synoviae is an economically significant pathogen in the poultry industry, inducing respiratory disease and infectious synovitis in chickens and turkeys, and eggshell apex abnormality in chickens. Eradication, medication and vaccination are the options for controlling M. synoviae infection. Currently there are two commercial, live, attenuated vaccines available against M. synoviae: the temperature sensitive MS-H vaccine strain and the NAD independent MS1 vaccine strain. Differentiation of vaccine strains from field isolates is essential during vaccination and eradication programs. The present study provides melt-curve and agarose gel based mismatch amplification mutation assays (MAMA) to discriminate the MS1 vaccine strain from the MS-H vaccine strain and wild-type M. synoviae isolates. The assays are based on the A/C single nucleotide polymorphism at nt11 of a HIT family protein coding gene. The melt- and agarose-MAMAs reliably distinguish the MS1 vaccine strain genotype from the MS-H vaccine strain and wild-type M. synoviae isolate genotype from 102 template number/DNA sample. No cross-reactions with other avian Mycoplasma species were observed. The assays can be performed directly on clinical samples and they can be run simultaneously with the previously described MAMAs designed for the discrimination of the MS-H vaccine strain. The developed assays are applicable in laboratories with limited facilities and promote the rapid, simple and cost effective differentiation of the MS1 vaccine strain.

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Identification of a new genetic marker in Mycoplasma synoviae vaccine strain MS-H and development of a strategy using polymerase chain reaction and high-resolution melting curve analysis for differentiating MS-H from field strains

Zhu

Konsak

Olaogun

et al. 2017

Veterinary Microbiology

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Variable lipoprotein haemagglutinin (vlhA) gene sequence typing of mainly DutchMycoplasma synoviaeisolates: comparison withvlhAsequences from Genbank and with amplified fragment length polymorphism analysis

Cited by 13 publications

References 43 publications

Development and evaluation of a multi-locus sequence typing scheme for Mycoplasma synoviae

Development and evaluation of a multi-locus sequence typing scheme for Mycoplasma synoviae

Development of mismatch amplification mutation assays for the differentiation of MS1 vaccine strain from wild-type Mycoplasma synoviae and MS-H vaccine strains

Identification of a new genetic marker in Mycoplasma synoviae vaccine strain MS-H and development of a strategy using polymerase chain reaction and high-resolution melting curve analysis for differentiating MS-H from field strains

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