Variable number tandem repeat analysis ofMycobacterium bovisisolates from Gyeonggi-do, Korea

Abstract

Bovine tuberculosis (TB) is a major zoonosis that's caused by Mycobacterium bovis (M. bovis). Being able to detect M. bovis is important to control bovine TB. We applied a molecular technique, the variable number tandem repeat (VNTR) typing method, to identify and distinguish the M. bovis isolates from Gyeonggi-do, Korea. From 2003 to 2004, 59 M. bovis clinical strains were isolated from dairy cattle in Gyeonggi-do, Korea, and these cattle had tuberculosis-like lesions. Twenty-four published MIRU-VNTR markers were applied to the M. bovis isolates and ten of them showed allelic diversity. The most discriminatory locus for the M. bovis isolates in Korea was QUB 3336 (h = 0.64). QUB 26 and MIRU 31 also showed high discriminative power (h = 0.35). The allelic diversity by the combination of all VNTR loci was 0.86. Six loci (MIRU 31, ETR-A and QUB-18, -26, -3232, -3336) displayed valuable allelic diversity. Twelve genotypes were identified from the 59 M. bovis isolates that originated from 20 cattle farms that were dispersed throughout the region of Gyenggi-do. Two genotypes [designation index (d.i.) = e, g] showed the highest prevalence (20% of the total farms). For the multiple outbreaks on three farms, two successive outbreaks were caused by the same genotype at two farms. Interestingly, the third outbreak at one farm was caused by both a new genotype and a previous genotype. In conclusion, this study suggests that MIRU-VNTR typing is useful to identify and distinguish the M. bovis isolates from Gyeonggi-do, Korea.

Isolation and Potential for Transmission ofMycobacterium bovisat Human-livestock-wildlife Interface of the Serengeti Ecosystem, Northern Tanzania

“…Findings from this study are consistent with earlier studies conducted by Jeon et al. (), Skuce et al. () and Smittipat et al.…”

The discovery, function and development of the variable number tandem repeats in differentMycobacteriumspecies

“…The genotyping of M. bovis is used mainly to investigate its transmission from domestic animals to human beings (Allix et al, 2006;Furphy et al, 2012;Hilty et al, 2005;Jeon et al, 2008;Price-Carter et al, 2011). For better discrimination, the spoligotyping method was recommended to be combined with VNTR (Lari et al, 2006;Martinez et al, 2008;McLernon et al, 2010;Parreiras et al, 2012;Rodriguez-Campos et al, 2013;Smith et al, 2003).…”

Mycobacterial Interspersed Repetitive Unit-variable Number Tandem Repeat (MIRU-VNTR) Typing Lacks Discriminatory Power in the Genetic Analysis of Bovine Tuberculosis in Egypt

“…The identification of M. bovis by conventional bacteriological techniques is time consuming. Therefore, molecular identification techniques (spoligotyping and mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) analysis) have been developed and were proved to be quicker and reliable in discriminating M. bovis from the other Mycobacterium tuberculosis complex [4][5][6][7][8][9][10][11]. Repeated sequences were identified in the genome of M. bovis and M. tuberculosis and were used to type M. bovis by MIRU-VNTR analysis [4][5][6][7][8][9][10][11].…”