Variable surface transport modalities on functionalized nylon films revealed with single molecule spectroscopy

Abstract

Functionalization of separation membranes with ion-exchange ligands allows control of the surface mobility of protein molecules facilitating optimized membrane design.

A mechanistic examination of salting out in protein–polymer membrane interactions

“…Additionally, circular dichroism (CD) spectroscopy is utilized to quantify structural changes of proteins at the stationary phase support and relate to TIRF and FPLC results. TIRF single-molecule tracking has been recently shown to reveal interfacial protein dynamics on a range of surfaces, including natural and synthetic polymers (44)(45)(46)(47)(48), and is ideal for measuring interfacial dynamics as it intrinsically suppresses background signal from emitters diffusing in the bulk solution. TIRF microscopy has been applied in many other systems including nanoparticle catalysis (49)(50)(51), DNA hybridization kinetics (52)(53)(54), and protein transport in live cells (54,55).…”

Optical Microscopic Techniques for Synthetic Polymer Characterization

“…These polymers are used to produce protein filtration membranes and cell culture dishes. As model molecules, fluorescently labeled lysozyme , and/or α-lactalbumin, ,, which have a positive and negative net charge at neutral pH, respectively, were used in addition to bovine serum albumin (BSA) and human monoclonal antibody (IgG) …”

“…Tauzin et al investigated the adsorption/desorption behavior of the charged protein molecules on nylon-6,6 surfaces grafted with charged polymers using SMT . They observed the electrostatic enhancement of the interfacial adsorption of lysozyme and α-lactalbumin on anionic and cationic polymer-grafted surfaces, respectively.…”

“…Dynamic Molecular Behavior at Synthetic Polymer Surfaces. SMT and STReM were employed to measure the detailed dynamic behavior of protein molecules at aqueous interfaces with thin films of synthetic polymers, PES blends, 105 nylon-6,6, 36,106,107 and PS. 108 These polymers are used to produce protein filtration membranes and cell culture dishes.…”

“…108 These polymers are used to produce protein filtration membranes and cell culture dishes. As model molecules, fluorescently labeled lysozyme 106,108 and/ or α-lactalbumin, 36,106,107 which have a positive and negative net charge at neutral pH, respectively, were used in addition to bovine serum albumin (BSA) and human monoclonal antibody (IgG). 105 Langdon et al employed single-molecule TIR fluorescence microscopy to study the adsorption behavior of fluorescently labeled BSA and IgG onto thin films of PES blended with PVP, PVAc-co-PVP, and PEGM.…”