VARIASI GENETIK IKAN BAUNG, Mystus nemurus DARl BEBERAPA WADUK DI JAWA YANG DIANALISIS DENGAN MARKER MITOKONDRIA D-LOOP

Nugroho, Estu; Hadie, Wartono; Sudarto, Sudarto

doi:10.15578/jppi.9.1.2003.1-5

Cited by 4 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar phenomena were observed in population variation analysis. Significant genetic differentiation was reported by Nugroho et al (2005) among four reservoir's H. nemurus in Java Island, whereas no significant genetic differentiation was also reported between H. nemurus populations from Jatiluhur and Wonogiri (Nugroho et al 2003).…”

Section: Introductionmentioning

confidence: 84%

“…It had reported that H. nemurus populations in Klawing and Serayu Rivers have a low genetic diversity as observed in their allozyme markers (Nuryanto and Setyaningrum 2008). A complex pattern of genetic diversity and population variations were reported in H. nemurus populations as assessed by RFLP markers of D-loop regions (Nugroho et al 2003;2005). In one hand, low genetic diversity was also observed in H. nemurus from Java and Sumatera as observed by RFLP markers of D-loop region (Nugroho et al 2003).…”

Section: Introductionmentioning

confidence: 99%

“…A complex pattern of genetic diversity and population variations were reported in H. nemurus populations as assessed by RFLP markers of D-loop regions (Nugroho et al 2003;2005). In one hand, low genetic diversity was also observed in H. nemurus from Java and Sumatera as observed by RFLP markers of D-loop region (Nugroho et al 2003). On the other hand, moderate-high genetic diversity was observed in four dam populations of H. nemurus in Java (Nugroho et al 2005).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic diversity assessment of Hemibagrus nemurus from rivers in Java Island, Indonesia using COI gene

2019

View full text Add to dashboard Cite

Abstract. Nuryanto A, Komalawati N, Sugiharto. 2019. Genetic diversity assessment of Hemibagrus nemurus from rivers in Java Island, Indonesia using COI gene. Biodiversitas 20: 2707-2717. Green catfish (Hemibagrus nemurus) is a popular freshwater fish that highly exploited in almost all the rivers in Java Island. The exploited population tends to have low genetic diversity. Meanwhile, separated populations might lead to a genetic difference among the river populations. This study aims to investigate the genetic diversity and population variation of H. nemurus collected at eleven rivers across Java Island. The analysis based on 465 bp fragment of the cytochrome c oxidase 1 gene from 140 individuals. Analysis of overall populations proved that H. nemurus had a high gene diversity (h= 0.935±0.016) and nucleotide diversity (π = 0.073±0.036). Within population analysis also showed that H. nemurus populations showed high levels of gene diversity (h= 0.338±0.128 to 1.000±0.022) and nucleotide diversity (π =0.001±0.001 to 0.071±0.038). Those diversity values indicated that H. nemurus had high level of genetic diversity, except for the Citanduy population. Population comparison showed that significant genetic differences observed among populations (p= 0.000 for both variance component and Fst-value). However, pairwise comparison analysis indicated complex pattern of population differentiation. The high genetic diversity and complex pattern of population differentiation have important implication for H. nemurus conservation in Java Island.

show abstract

Section: Introductionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic diversity assessment of Hemibagrus nemurus from rivers in Java Island, Indonesia using COI gene

2019

View full text Add to dashboard Cite

show abstract

“…Syarat awal yang diperlukan untuk menentukan kekerabatan yang dimiliki adalah dengan pengumpulan informasi dan data dasar genetik suatu spesies. (Nugroho et al, 2003). Sampai saat ini belum ada catatan ilmiah mengenai informasi identifikasi larva ikan dengan sekuen DNA di perairan Danau Ranau, Sumatera Selatan.…”

Section: Pendahuluanunclassified

Identifikasi Jenis Dan Hubungan Kelimpahan Larva Ikan Dengan Kualitas Air Di Danau Ranau, Sumatera Selatan

Wulandari¹,

Herlan²,

Wibowo³

et al. 2019

BAWAL Widya Riset Perikanan Tangkap

View full text Add to dashboard Cite

ABSTRAKIdentifikasi larva ikan seringkali sulit dilakukan. Belum tersedianya kunci identifikasi untuk spesies larva ikan di perairan umum daratan sehingga belum adanya pedoman untuk dapat mengidentifikasi spesies larva ikan secara morfologi maupun meristik. Penelitian ini bertujuan untuk mengidentifikasi spesies larva ikan melalui sekuen DNA sehingga menghasilkan data spesies yang lebih pasti dan hubungan kelimpahan larva ikan dengan parameter kualitas perairan di Danau Ranau, Sumatera Selatan. Lokasi pengambilan sampel identifikasi jenis dan kualitas perairan di enam stasiun di Perairan Danau Ranau, Sumatera Selatan. Hasil identifikasi dengan menggunakan sekuen DNA ditemukan sepuluh jenis larva ikan. Kesepuluh jenis larva ikan tersebut antara lain Rasbora argyrotaenia, Puntius tetrazona, Oreochromis mossambicus, Oreochromis niloticus, Gambusia affinis, Poecilia reticulata, Mystacoleucus marginatus, Channa striata, Trichopsis vittata, and Trichogaster trichopterus. Sekuen DNA kemudian disejajarkan dengan sekuen referen dari perpustakaan data bank gen untuk mengakar pohon dengan menggunakan program BLAST dari NCBI untuk menemukan identitas yang paling dekat keterkaitannya dengan sampel larva-larva ikan yang dianalisis. Hubungan filogeni spesies diantara takson larvalarva ikan di Danau Ranau berdekatan. Kondisi perairan di Danau Ranau tergolong baik untuk biota perairan seperti larva ikan. Kualitas air merupakan parameter yang menentukan kelimpahan larva ikan di Danau Ranau. Larva-larva ikan antar populasi memiliki jarak genetik yang dekat antar satu dengan yang lain. Kelimpahan larva ikan berkorelasi positif dengan turbiditas dan berkorelasi negatif terhadap parameter daya hantar listrik. Kata Kunci: Larva ikan; Kualitas perairan; Sekuen DNA; Danau Ranau ABSTRACTIdentification of fish larva is often difficult. The unavailability of key identification for fish larva species in inland waters so that there are no guidelines for identifying fish larvae morphologically or meristically. This study aims to identify fish larva species from the Ranau Lake waters, South Sumatra through DNA sequences to produce more definitive species data and relationship of abundance of fish larva and water quality of parameters in Ranau Lake waters, South Sumatera. The sampling location of species identification and water quality was six stations in Ranau Lake waters, South Sumatra. The results of the study found the identification of fish larva species using DNA sequence found ten types of fish larva obtained. The ten types of fish larva include Rasbora argyrotaenia, Puntius tetrazona, Oreochromis mossambicus, Oreochromis niloticus, Gambusia affinis, Poecilia reticulata, Mystacoleucus marginatus, Channa striata, Trichopsis vittata, and Trichogaster trichopterus. The DNA sequence was then aligned with the referent sequence from the gene bank data library to root the tree by using the BLAST program from NCBI to find the identity that was closest to the sample of fish larvae analyzed. The relationship of species phylogeny between the fish l...

show abstract

“…Penyediaan benih untuk pembesaran sebagian besar masih mengandalkan tangkapan dari alam. Tujuan budidaya adalah untuk peningkatan produksi dan efisiensi (Nugroho et al, 2003). Oleh sebab itu, agar produksi dapat dilakukan secara optimal dan sesuai harapan maka dibutuhkan induk-induk yang berkualitas baik dan bagus untuk dikembangkan supaya dapat menghasilkan gonad yang berkualitas.…”

Section: Pendahuluanunclassified

PERTUMBUHAN DAN PERKEMBANGAN GONAD IKAN BAUNG (Hemibagrus nemurus) KETURUNAN G-1, G-2, DAN G-3

Kusmini

Kristanto

Widiyati

et al. 2019

Jurnal Riset Akuakultur

View full text Add to dashboard Cite

Ikan baung (Hemibagrus nemurus) telah berhasil didomestikasi oleh Balai Riset Perikanan Budidaya Air Tawar dan Penyuluhan Perikanan, Bogor. Hasil penelitian domestikasi, populasi Cirata memiliki fekunditas dan pertumbuhan yang lebih bagus, populasi ini dikembangkan hingga tiga generasi (G1, G2 dan G3) namun, ada informasi terbatas mengenai perbandingan antara perkembangan gonad dari berbagai generasi ikan. Tujuan penelitian ini adalah untuk mengukur pertumbuhan dan mengamati perkembangan gonad generasi pertama (G-1), kedua (G-2), dan ketiga (G-3). Parameter yang diukur dan diamati meliputi pengukuran panjang, berat, laju pertumbuhan spesifik, morfologi dan berat gonad, diameter oosit, dan indeks gonad somatik (IGS). Pengambilan sampel dilakukan setiap dua bulan dengan mengumpulkan sepuluh sampel ikan dari setiap generasi. Jaringan gonad disiapkan secara histologis dan diwarnai larutan asetokarmin sebagai zat pewarna. Pengamatan menggunakan mikroskop dengan perbesaran 4 x 10. Hasil penelitian menunjukkan bahwa pada usia 222 hari, gonad jantan dan betina secara fisik masih dalam bentuk benang halus. Namun, karakteristik sekunder pada ikan jantan seperti papila sudah mulai tampak meski tidak terlalu jelas. Pada usia 283 hari, ukuran oosit berkisar antara 0.025-0.05 mm. Pada usia 345 hari, telur mulai terlihat dengan ukuran oosit G-1, G-2, dan G-3 masing-masing berkisar antara 0.59 ± 0.24, 0.39 ± 0.15, dan 0.48 ± 0.15 mm. Setelah usia 406 hari, perkembangan gonad mencapai TKG-III dengan diameter rata-rata oosit G-1, G-2, dan G-3 berkisar antara 1.13 ± 0.11, 0.92 ± 0.18, dan 1.11 ± 0.10 mm dengan IGS dari G-1, G-2, dan G-3 dari 8.20%, 0.98 %, dan 4.8%, masing-masing. Tidak ada perbedaan yang signifikan antara diameter oosit G-1, G-2, dan G-3 (P£0,05). Keturunan kedua (G-2) merupakan generasi terbaik untuk dijadikan kandidat budidaya. Asian redtail catfish (Hemibagrus nemurus) has been successfully domesticated by the Research Institute for Freshwater Aquaculture and Fisheries Extension, Bogor. The results of the domestication of Cirata population have better fecundity and growth, this population has been developed for up to three generations (G1, G2 and G3) however, there is limited information regarding the comparison between the ovarian development of different generations of the fish]. This study’s aim was to measure the growth and observe the ovarian development of the first (G-1), second (G-2), and third generations (G-3). The parameters measured and observed included the measurements of length, weight, specific growth rate, morphology and gonad weight, oocyte diameter, and somatic gonad index (IGS). The sampling was carried out every two months by collecting ten fish samples of each generation. Gonad tissues were histologically prepared and stained asetocarmine solution as the coloring agent. The observations used a microscope with a magnification of 4 x 10. The results of the study showed that at the age of 222 days, the male and female gonads were physically still in the form of fine threads. However, secondary characteristics in male fish such as papillae have begun to appear though not very clear. At the age of 283 days, oocytes’ sizes ranged between 0.025-0.05 mm. At the age of 345 days, the eggs were starting to be visible with the oocyte sizes of G-1, G-2, and G-3 ranged between 0.59 ± 0.24, 0.39 ± 0.15, and 0.48 ± 0.15 mm, respectively. After the age of 406 days, the development of gonads reached gonad maturating level III with the average diameter of the oocytes of G-1, G-2, and G-3 ranged between 1.13 ± 0.11, 0.92 ± 0.18, and 1.11 ± 0.10 mm with the IGS of G-1, G-2, and G-3 of 8.20%, 0.98%, and 4.8%, respectively. There was no significant difference among the oocyte diameters of G-1, G-2, and G-3 (P£0.05). The 2nd generation (G2) is the best generation to be a candidate aquaculture.

show abstract

VARIASI GENETIK IKAN BAUNG, Mystus nemurus DARl BEBERAPA WADUK DI JAWA YANG DIANALISIS DENGAN MARKER MITOKONDRIA D-LOOP

Abstract: Variasi genetik beberapa ras ikan baung yang dikoleksi dari Jatiluhur, Cirata, Wonogiri, dan Wadaslintang telah diteliti dengan menggunakan polimorfisme mitokondria DNA D-loop.

Cited by 4 publications

References 0 publications

Genetic diversity assessment of Hemibagrus nemurus from rivers in Java Island, Indonesia using COI gene

Genetic diversity assessment of Hemibagrus nemurus from rivers in Java Island, Indonesia using COI gene

Identifikasi Jenis Dan Hubungan Kelimpahan Larva Ikan Dengan Kualitas Air Di Danau Ranau, Sumatera Selatan

PERTUMBUHAN DAN PERKEMBANGAN GONAD IKAN BAUNG (Hemibagrus nemurus) KETURUNAN G-1, G-2, DAN G-3

Contact Info

Product

Resources

About