Variation in anthocyanins and total phenolics of black raspberry populations

Characterization of a novel anthocyanin profile in wild black raspberry mutants: An opportunity for studying the genetic control of pigment and color

“…Fruit were harvested from this plot in July 2010 for analysis of anthocyanins and other maturity traits (data not shown). Protocols used for fruit harvest and sample preparation were similar to those previously described (Dossett et al, 2008(Dossett et al, , 2010. Briefly, 25 berries from each plant (genotype) were picked, weighed, and added to a bulk fruit sample of the population for each plot.…”

“…Black raspberry fruit is dominated by cyanidin-3-rutinoside and cyanidin-3-xylosylrutinoside, which account for 80% or more of the total anthocyanins (Dossett, Lee, & Finn, 2010;Hong & Wrolstad, 1990a;Ozgen et al, 2008;Tian et al, 2006aTian et al, , 2006bTulio et al, 2008;Wyzgoski et al, 2010). These two main anthocyanins are also more potent phenolic antioxidants (cyanidin-3-xylosylrutinoside > cyanidin-3-rutinoside) compared to the other anthocyanins present in black raspberry fruit , though limited information is available about the potential bioactivity of individual cyanidin-based anthocyanins with different sugar moieties (Tian et al, 2006b;Tulio et al, 2008;Stintzing, Stintzing, Carle, Frei, & Wrolstad, 2002;Stoner et al, 2005) or their relative desirability for product development, food processing, natural colorant usage, and storability (Hager, Howard, Prior, & Brownmiller, 2008;Hong & Wrolstad, 1990a;Stintzing et al, 2002).…”

“…While many of the enzymes involved in anthocyanin biosynthesis have been identified in model plant systems (Petunia · hybrida Hort., Antirrhinum majus L., and Zea mays L.), none of the genes involved in anthocyanin production have been mapped or identified in black raspberry. This is made difficult by the fact that, while anthocyanin deficient (yellow/orange-fruited) mutants have been recognized for more than a century (Card, 1898) and differences in the relative proportion of anthocyanins have been reported (Dossett et al, 2008(Dossett et al, , 2010, little variation in the types of anthocyanins present in black raspberry fruit has been found. While a major effort to identify and map genes involved in anthocyanin biosynthesis and fruit color is underway in red raspberry (Kassim et al, 2009;McCallum et al, 2010), the apparent lack of variation in cultivated black raspberry has limited similar work in this crop.…”

Developing spray-dried powders containing anthocyanins of black raspberry juice encapsulated based on fenugreek gum

“…This fruit due to the presence of anthocyanins is known for some biological functions such as, reducing eyestrain, DNA repair, anticancer, anti-neurodegenerative, and anti-inflammatory activities [2,3]. The anthocyanins stability is undesirably affected by environmental conditions including temperature, pH, oxygen presence, light, ascorbic acid, sugars and metal ions and co-pigments belonging to the various compounds classes [4].…”

Rubus occidentalis (Rosaceae) – a new naturalized raspberry species in the Polish flora

“…The species has relatively modest commercial production when compared to red raspberries or blackberries. Nevertheless, demand for black raspberry fruit has been growing in recent years because of the health benefits resulting from a particularly high content of anthocyanins, and it is now cultivated in many parts of the world with temperate climates (Kresty et al 2006;Dossett et al 2010).…”