Variation in Antioxidant Activity and Flavonoid Aglycones in Eggplant (Solanum melongena L.) Germplasm

Piao, Xiang Min; Chung, Jong‐Bae; Lee, Gi An; Lee, Jung Ro; Cho, Gyu Taek; Lee, Ho-Sun; Ho, Kyung; Guo, Jing; Kim, Hong Sig; Lee, Sok Young

doi:10.9787/pbb.2014.2.4.396

Cited by 13 publications

(11 citation statements)

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“…Our results on the peel extracts from DR2 aubergine berries partly agree with literature evidence, mainly regarding the hydroxycinnamic acid composition, while the flavones apigenin and naringenin were firstly identified. Naringenin was also found to be transported and incorporated in the outer cuticle matrix [ 53 ], while apigenin was reported abundant in eggplant leaves [ 54 ].…”

Section: Resultsmentioning

confidence: 99%

A Polyphenol Rich Extract from Solanum melongena L. DR2 Peel Exhibits Antioxidant Properties and Anti-Herpes Simplex Virus Type 1 Activity In Vitro

et al. 2018

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DR2B and DR2C extracts, obtained by ethanolic maceration of peel from commercially and physiologically ripe aubergine berries, were studied for the antioxidative cytoprotective properties and anti-HSV-1 activity, in line with the evidence that several antioxidants can impair viral replication by maintaining reducing conditions in host cells. The antioxidative cytoprotective effects against tBOOH-induced damage were assessed in Caco2 cells, while antiviral activity was studied in Vero cells; polyphenolic fingerprints were characterized by integrated phytochemical methods. Results highlighted different compositions of the extracts, with chlorogenic acid and delphinidin-3-rutinoside as the major constituents; other peculiar phytochemicals were also identified. Both samples reduced reactive oxygen species (ROS) production and exhibited scavenging and chelating properties. DR2C partly counteracted the tBOOH-induced cytotoxicity, with a remarkable lowering of lactate metabolism under both normoxia and hypoxia; interestingly, it increased intracellular GSH levels. Furthermore, DR2C inhibited the HSV-1 replication when added for 24 h after viral adsorption, as also confirmed by the reduction of many viral proteins’ expression. Since DR2C was able to reduce NOX4 expression during HSV-1 infection, its antiviral activity may be correlated to its antioxidant properties. Although further studies are needed to better characterize DR2C activity, the results suggest this extract as a promising new anti-HSV-1 agent.

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Section: Resultsmentioning

confidence: 99%

A Polyphenol Rich Extract from Solanum melongena L. DR2 Peel Exhibits Antioxidant Properties and Anti-Herpes Simplex Virus Type 1 Activity In Vitro

et al. 2018

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“…Study by Kandoliya et al [15] figured that methanolic pulp extract of sample GBL-1 showed the highest TFC (13.25 mg QE/100 g) among six varieties of eggplant. While Piao et al [16] reported that total flavonoid aglycone in sample from 45°N location was the highest compared to other location. It was similar to sample that collected at ≤£80 days in flowering presented the highest total flavonoid aglycone.…”

Section: Discussionmentioning

confidence: 99%

“…The other reasons may be different solvent for extraction process, different site location, etc. The previous study [16], demonstrated that 75% ethanolic leaves extract of different harvest time and different site location gave different result in antioxidant activities by DPPH and ABTS methods. The previous result revealed that sample which collected at ≤80 days in flowering gave the highest antioxidant activity by DPPH method (53.1 µg L-ascorbic acid equivalent (ASC)/mg extract) and ABTS method (73.9 µg Trolox/mg), while sample from 45°N location exhibited the highest antioxidant by DPPH 56.5 µg ASC/mg extract and the highest by ABTS method (75.6 µg Trolox/mg) compared to the others sample.…”

Section: Discussionmentioning

confidence: 99%

“…The previous researchers [15,16] revealed that eggplant (S. melongena) had antioxidant capacity. There was no research regarding the antioxidant activity of different organs of eggplant (S. melongena) which were leaves, fruit, and stem extracted using increasing polarity solvents (n-hexane, ethyl acetate, and ethanol) and tested by DPPH and FRAP assays.…”

Section: Discussionmentioning

confidence: 99%

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Phytochemical Content and Antioxidant Potential of Different Organs of Eggplant (Solanum Melongena L.) Grown in West Java-Indonesia

Fidrianny

Winarsih

Ruslan

2017

Asian J Pharm Clin Res

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Objectives: The goals of this research were to evaluate antioxidant potential from different organs of eggplant (Solanum melongena L.) using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) and correlation of total phenolic and flavonoid content with their inhibitory concentration 50% (IC 50 ) of DPPH, and exhibitory concentration 50% (EC 50 ) of FRAP. Materials and Methods:Each sample was extracted by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant activities were tested using DPPH and FRAP assays, determination of total phenolic and flavonoid content were carried out by ultravioletvisible spectrophotometry and correlation with their IC 50 of DPPH and EC 50 of FRAP capacities were analyzed by Pearson's method. Results:The lowest IC 50 of DPPH scavenging activity 1.14 µg/ml and the lowest EC 50 of FRAP capacity 49.80 µg/ml was given by ethanolic leaves extract of eggplant. Ethanolic leaves extract of eggplant also presented the highest total phenolic content (TPC) (8.87 g gallic acid equivalent/100 g), while the highest total flavonoid content was shown by ethyl acetate leaves extract (24.50 g quercetin equivalent/100 g). There was a significantly negative correlation between TPC in leaves and fruit extracts of eggplant with their IC 50 of DPPH and EC 50 of FRAP. Conclusions:All different extracts of eggplant organs (except n-hexane stem extract) were categorized as a very strong antioxidant by DPPH method. Phenolic compounds in eggplant leaves and fruit extracts were the major contributor in antioxidant activities by DPPH and FRAP methods. DPPH and FRAP showed linear results in antioxidant activities of eggplant leaves, fruit and stem extracts.

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“…Our peel extracts partly agree with literature evidence, mainly regarding the hydroxycinnamic acid composition, while the flavones apigenin and naringenin were identified. Naringenin was also found to be transported and incorporated in the outer cuticle matrix [53], while apigenin was reported abundant in eggplant leaves [54]. [34].…”

Section: Phytochemical Analysismentioning

confidence: 99%

A Polyphenol Rich Extract from Solanum melongena L. DR2 Peel Exhibits Antioxidant Properties and Anti-Herpes Simplex virus Type 1 Activity In Vitro

Sotto

Giacomo

Amatore

et al. 2018

Preprint

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DR2B and DR2C extracts, from peel of commercially and physiologically ripe eggplants, were studied for the antioxidative cytoprotective properties and anti-HSV-1 activity, in line with the evidence that several antioxidants can impair viral replication by maintaining reducing conditions into the host cells. The antioxidative cytoprotective effects against tBOOH-induced damage was assessed in Caco2 cells, while the antiviral activity was studied in Vero cells; phenolic and anthocyanin fingerprint was characterized by integrated phytochemical methods. Results highlighted different compositions of the extracts, with chlorogenic acid and delphinidin-3-rutinoside as the major constituents; other peculiar phytochemicals were also identified. DR2C resulted able to partly counteract the tBOOH-induced cytotoxicity, with a remarkable lowering of lactate metabolism under both normoxia and hypoxia. DR2B and DR2C reduced ROS production, possessed scavenging and chelating properties. Interestingly, DR2C increased intracellular GSH levels. Furthermore, DR2Cinhibited the HSV-1 replication when added for 24 h after viral adsorption, as also confirmed by the reduction of many viral proteins expression. Since DR2C was able to reduce NOX4 expression during HSV-1 infection, its antiviral activity may be correlated to its antioxidant properties. Although further studies are needed to better characterize DR2C activity, the results suggest this extract as a promising new anti-HSV-1 agent.

show abstract

Variation in Antioxidant Activity and Flavonoid Aglycones in Eggplant (Solanum melongena L.) Germplasm

Cited by 13 publications

References 9 publications

A Polyphenol Rich Extract from Solanum melongena L. DR2 Peel Exhibits Antioxidant Properties and Anti-Herpes Simplex Virus Type 1 Activity In Vitro

A Polyphenol Rich Extract from Solanum melongena L. DR2 Peel Exhibits Antioxidant Properties and Anti-Herpes Simplex Virus Type 1 Activity In Vitro

Phytochemical Content and Antioxidant Potential of Different Organs of Eggplant (Solanum Melongena L.) Grown in West Java-Indonesia

A Polyphenol Rich Extract from <em>Solanum melongena</em> L. DR2 Peel Exhibits Antioxidant Properties and Anti-Herpes Simplex virus Type 1 Activity <em>In Vitro</em>

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