Variation in lettuce plants regenerated from protoplasts

Engler, Dean E.; Grogan, R. G.

doi:10.1093/oxfordjournals.jhered.a109978

Cited by 42 publications

(22 citation statements)

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“…Also, 42% of the primary regenerants exhibited some loss in fertility, the genetic basis of which is not known. Engler and Grogan [6] have reported that lettuce plants regenerated from leaf mesophyll protoplasts frequently show similar infertility.…”

Section: Discussionmentioning

confidence: 98%

Genetic instability during embryogenic cloning of celery

Orton¹

1985

Plant Cell Tiss Organ Cult

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Genetically marked tissues of celery (Apium graveolens) were employed to contrast genetic and chromosomal stability in serially bulk-transferred callus and regenerated plants. After six months in culture, 84% of the callus cells were karologically indistinguishable from normal, while the remainder exhibited chromosome loss and/or fusion. All of 50 clones derived from this tissue expressed the control phenotype with respect to heterozygous isozyme markers. Of 95 plants regenerated from the same tissue, 94 were phenotypieally indistinguishable from the original explant donor, and cytogeneric analyses revealed the presence in 4.3% of an accessory chromosome, while the remainder were normal diploids. Analysis of the selfed progeny of these regenerated plants revealed the presence of a new recessive mutation causing abnormal leaf morphology at a frequency of 1.8%. Only one of 40 cells in 12-month-old callus tissue was karyologicaily indistinguishable from normal, the remainder consisting primarily of hypodiploids. The observation that aU 50 clones were phenotypicaLly heterozygous was statistically inconsistent with the hypothesis that hypodiploidy was associated with random complete chromosome loss. The culture had, at this point, lost the ability to regenerate. It is speculated that embryogenic cloning of celery may be suitable under certain circumstances for direct field establishment, but that levels of new genetic variation are sufficiently high to preclude its use for seed production.

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Section: Discussionmentioning

confidence: 98%

Genetic instability during embryogenic cloning of celery

Orton¹

1985

Plant Cell Tiss Organ Cult

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“…Protoc1onal variation also was reported for rice (Abdullah et al, 1989;Ogura et al, 1987) and several other crops (Engler and Grogan, 1984;Lörz and Scowcroft, 1983;Shahin and Spivey, 1986;Shepard et al, 1980). The field performance of 125 R1 plants regenerated from protoplasts of four japonica cultivars was reported by Ogura et al , (1987).…”

Section: Useful Clonal Variation In Cereal Cropsmentioning

confidence: 78%

Clonal Variation in Cereals and Forage Grasses

Rush

Xie

Croughan

et al. 1998

Somaclonal Variation and Induced Mutations in Crop Improvement

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“…Selection of somaclonal variants has successfully been used to generate cultivars in a number of plants, including apple (Donovan et al 1994), banana (Cote et al 1993), celery (Heath-Pagliuso et al 1988), cucumber (Burza and Malepszy 1995;Filipecki et al 2005), garlic (Novak et al 1982), lettuce (Engler and Grogan 1984), peach (Hammerschlag 1990), strawberry (Swartz et al 1981;Toyoda et al 1991;Takahashi et al1992;Hammerschlag et al 2006), and tomato (Evans and Sharp 1983;Barden et al 1986). Although it is important to use a regeneration method that results in a high frequency of somaclonal variation in the regenerated plants so as to efficiently select useful somaclonal variants (Ezura et al 1995), in Japanese butterbur, it was difficult to control the high frequency occurrence of undesirable variation, which was observed in the regenerants from callus cultures (Morishita 1991).…”

Section: ϫ2mentioning

confidence: 99%

Breeding of Japanese butterbur (Petasites japonicus) by using flowerhead culture

Iwamoto

2009

Plant Biotechnology

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Variation in lettuce plants regenerated from protoplasts

Cited by 42 publications

References 0 publications

Genetic instability during embryogenic cloning of celery

Genetic instability during embryogenic cloning of celery

Clonal Variation in Cereals and Forage Grasses

Breeding of Japanese butterbur (Petasites japonicus) by using flowerhead culture

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