Variation in Metabolism of Biochemical Test Substrates by Klebsiella Species: An Epidemiological Tool

Barr, J. G.

doi:10.1099/00222615-11-4-501

Cited by 5 publications

(3 citation statements)

References 7 publications

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“…evidence presented suggests that sufficient discrimination may be obtained by the former method alone. The reproducibility of the techniques employed has been evaluated (Barr, 1978). Repeat samples of urine from the same patient normally yielded the same biotype, and the method allowed the detection of occasional mixed biotypes in catheter specimens.…”

Section: Discussionmentioning

confidence: 99%

“…However, not infrequently and especially in catheterised patients, different biotypes were isolated from the same patient on successive occasions; these changes were often accompanied by increased or decreased antibiotic sensitivity, and by recognisable colonial changes. Barr (1978) and Rennie and Duncan (1974) have previously shown that changes in biotype could occur, especially in patients with tracheostomies, and the latter authors showed that biotype changes were often accompanied by changes in capsular serotype.…”

Section: Multiple Biotypes In Urine: Biotypes In Repeat Samplesmentioning

confidence: 94%

“…Barr (1978) evaluated the reproducibility of the methods used in the discrimination among klebsiella isolates by differential substrate metabolism. When the same methods were applied in the biotyping of E. aerogenes, uniform results were obtained when clinical isolates were typed on four successive days.…”

Section: Multiple Biotypes In Urine: Biotypes In Repeat Samplesmentioning

confidence: 99%

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Biotypes of Klebsiella pneumoniae (sensu lato) and Enterobacter aerogenes characterised by differential substrate metabolism: application of the technique.

Barr¹,

Hogg²

1979

J Clin Pathol

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SUMMARY A biochemical typing method is described for Klebsiella pneumoniae (sensu lato) and Enterobacter aerogenes. The technique depends on differences in metabolism of five carbon substrates-glycerol, inositol, lactose, glucose, and xylose-at two concentrations. Reproducibility is satisfactory and is monitored by the incorporation of control klebsiellae of known biotype.The method has been used for 12 months in the surveillance of urinary tract colonisation in this hospital. Gut carriage of klebsiellae, implicated by several workers as a source of infection, was common among staff and new admissions. Many biotypes were represented which were sensitive to most antibiotics except ampicillin.Klebsiellae, all multiply resistant, were isolated most frequently from urine specimens in two orthopaedic wards. In a longitudinal study in these wards, a sequential dominance in urinary tract colonisation by two klebsiella biotypes was shown, which suggested the presence of cross infection or an environmental reservoir. Confirmatory evidence was obtained from capsular serotypes and R-factor studies.Klebsiellae are one of the major causes of hospital acquired infection. This has led to the development of many methods which aimed to detect identical strains of the same species and thus provide a reliable method for epidemiological studies.Serological (Casewell, 1972;Riser et al., 1976; Palfreyman,1978) Received for publication 7 March 1979 Biochemical typing, however, if feasible, would be quick and inexpensive and could overcome the requirements for special reagents and special expertise often needed by other methods. Barr (1978) identified a number of biochemical test substrates whose differential metabolism provided good discrimination between isolates of klebsiella. In our experience, differential metabolism of these substrates also allowed discrimination between isolates of Enterobacter aerogenes. The various biotypes of Klebsiella pneumoniae (sensu lato) and Enterobacter aerogenes distinguished by the differential metabolism of five selected substrates are described here.This technique is applied to surveillance of biochemical types of klebsiella associated with colonization of the urinary tract. The results suggest that the preponderance of certain biotypes in some wards could be attributed to cross infection or to a common source. Material and methods ORGANISMS: ISOLATION AND IDENTIFICATIONA total of 445 clinical isolates of klebsiellae were examined over a 12-month period. These included 935 on 12 May 2018 by guest. Protected by copyright.

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Section: Discussionmentioning

confidence: 99%

Section: Multiple Biotypes In Urine: Biotypes In Repeat Samplesmentioning

confidence: 94%

Section: Multiple Biotypes In Urine: Biotypes In Repeat Samplesmentioning

confidence: 99%

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Biotypes of Klebsiella pneumoniae (sensu lato) and Enterobacter aerogenes characterised by differential substrate metabolism: application of the technique.

Barr¹,

Hogg²

1979

J Clin Pathol

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The Genus Klebsiella

Brisse¹,

Grimont²,

Grimont³

2006

The Prokaryotes

147

138

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Effects of organic nutrients and growth factors on biostimulation of tributyltin removal by sediment microorganisms and Enterobacter cloacae

Sakultantimetha

Keenan

Beattie

et al. 2010

Appl Microbiol Biotechnol

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Natural attenuation can reduce contamination of tributyltin (TBT), but persistence of the xenobiotic can cause long-term issues in the environment. Biostimulation is used to accelerate biodegradation. This study investigated the ability of individual organic nutrients and growth factors to enhance TBT biodegradation by sediment microorganisms (SED) and Enterobacter cloacae strain TISTR1971 (B3). The supplements that produced high biomass yield were selected for degradation enhancement. For TBT degradation at initial concentration of 0.1 mg/l, negative or limited degradation was observed in some selected supplements indicating that increasing the biomass did not necessarily promote degradation. Consequently, the addition of nutrients was expected to increase both dioxygenase activity and the degrader population. At different concentrations of supplements, a mixture of succinate/glycerol showed the highest removal for SED which reduced TBT by 77%, 75%, and 68% for 0.1×, 1×, and 10× supplement concentration, respectively. For B3, the addition of succinate showed degradation of 49% (0.1×), 75% (1×), and 77% (10×). Most nutrients and amino acids had an inhibitory effect at 1× or 10× levels. Excess amount of the nutrients added can inhibit the initial degradation of TBT. Therefore, TBT biostimulation requires supplements that increase the capability of TBT degraders at an appropriate amount.

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Variation in Metabolism of Biochemical Test Substrates by Klebsiella Species: An Epidemiological Tool

Cited by 5 publications

References 7 publications

Biotypes of Klebsiella pneumoniae (sensu lato) and Enterobacter aerogenes characterised by differential substrate metabolism: application of the technique.

Biotypes of Klebsiella pneumoniae (sensu lato) and Enterobacter aerogenes characterised by differential substrate metabolism: application of the technique.

The Genus Klebsiella

Effects of organic nutrients and growth factors on biostimulation of tributyltin removal by sediment microorganisms and Enterobacter cloacae

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