Variation in nodulation and N2 fixation by the Gliricidia sepium/Rhizobium spp. symbiosis in a calcareous soil

Sanginga, N.; Manrique, K.; Hardarson, G.

doi:10.1007/bf00335847

Cited by 23 publications

(9 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results on nodulation test demonstrate that G. sepium preferentially forms nodules with strains of the Rhizobium genus which is in accordance with the observations of Halliday & Somasegaran (1983), Sanginga et al (1991), Turk & Keyser (1992), Moreira et al (1993), de Lajudie et al (1994), Dommergues et al (1998) who described G. sepium as preferentially forming efficient nitrogen-fixing symbioses with fast-growing rhizobia.…”

Section: Discussionsupporting

confidence: 94%

“…For this reason, it is important to continue to widen the collection of strains able to form efficient symbioses with G. sepium under differing soil conditions, as has already been done for Calliandra calothyrsus (Lesueur et al 1996(Lesueur et al , 2001. From a symbiotic point of view, G. sepium has been described as being a species having a specificity for symbiosis with fast-growing rhizobia (Halliday & Somasegaran 1984;Sanginga et al 1991;Moreira et al 1993;De Lajudie et al 1994). However, Awonaike et al (1992) and Ndiaye & Ganry (1997) showed that G. sepium could also be nodulated by slowgrowing rhizobia.…”

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

Diversity and effectiveness of rhizobial strains from Gliricidia sepium native to Reunion Island, Kenya and New Caledonia

Thiao

Neyra

Isidore³

et al. 2004

World J Microbiol Biotechnol

View full text Add to dashboard Cite

The aim of this study was to use several approaches to assess the diversity occuring in a collection of rhizobial strains that had been isolated from Gliricidia sepium (Jacq.) Walp. originating from Kenya, Reunion Island and New Caledonia. Results showed that G. sepium establishes an effective symbiosis only with fast-growing rhizobia. This suggests that this legume nodulates preferentially with this type of rhizobia, although ineffective nodules were observed on root systems of plants inoculated with slow-growing rhizobia. It was possible to distinguish several groups of strains which have the same behaviour in terms of utilization of amino acids and sources of carbon and intrinsic resistance to antibiotics and salinity. Six molecular groups were distinguished by profiles obtained using PCR/RFLP techniques. No relationship was demonstrated between phenotypic groups and molecular groups. Neither was it possible to find a relationship between the geographic origins of the strains and their distribution in the several phenotypic and/or molecular groups. In an experiment carried out under glasshouse conditions, we observed that all strains from the collection significantly improved the growth of G. sepium cultivated in a substrate totally devoid of nitrogen. The strain GsK5 induced the greatest stimulation of plant growth.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Introductionmentioning

confidence: 96%

Diversity and effectiveness of rhizobial strains from Gliricidia sepium native to Reunion Island, Kenya and New Caledonia

Thiao

Neyra

Isidore³

et al. 2004

World J Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Similar variations in the proportions and amounts of N2 fixed have been observed for G. sepium growing under identical conditions. Percentage Ndfa ranged from 26 to 68% between the 25 provenances examined (Sanginga et al, 1991b). Such variations were confirmed in field conditions although values were lower than in pot experiments (Sanginga et al, 1994b).…”

Section: Tree Host Provenance or Genotypic Variation In N2 Fixationmentioning

confidence: 54%

Management of biological N2 fixation in alley cropping systems: Estimation and contribution to N balance

1995

Self Cite

View full text Add to dashboard Cite

Residue N use efficiency in alley cropping systems Definitions Measurement of % N use efficiency Nitrogen not taken up by the crop in alley cropping Management practices for an increased N use efficiency in alley cropping systems Acknowledgements References AbstractAlley cropping is being widely tested in the tropics for its potential to sustain adequate food production with low agricultural inputs, while conserving the resource base. Fast growth and N yield of most trees used as hedgerows in alley cropping is due greatly to their ability to fix N2 symbiotically with Rhizobium. Measurements of biological N2 fixation (BNF) in alley cropping systems show that some tree species such as Leucaena leucocephala, Gliricidia sepium and Acacia mangium can derive between 100 and 300 kg N ha-l yr-l from atmospheric N2, while species such as Faidherbia albida and Acacia senegal might fix less than 20 kg N ha-1 yr-i. Other tree species such as Senna siamea and S. spectabilis are also used in alley cropping, although they do not nodulate and therefore do not fix N2. The long-term evaluation of the potential or actual amounts of N2 fixed in trees however, poses problems that are associated with their perennial nature and massive size, the great difficulty in obtaining representative samples and applying reliable methodologies for measuring N2 fixed. Strategies for obtaining representative samples (as against the whole tree or destructive plant sampling), the application of 15N procedures and the selection criteria for appropriate reference plants have been discussed.Little is known about the effect of environmental factors and management practices such as tree cutting or pruning and residue management on BNF and eventually their N contribution in alley cropping. Data using the 15N labelling techniques have indicated that up to 50% or more of the tree's N may be below ground after pruning. In this case, quantification of N2 fixed that disregards roots, nodules and crowns would result in serious errors and the amount of N2 fixed may be largely underestimated. Large quantities of N are harvested with hedgerow prunings (> 300 kg N ha-1 yr-1) but N contribution to crops is commonly in the range of 40-70 kg N ha-l season. This represents about 30% of N applied as prunings; however, N recoveries as low as 5-10% have been reported. The low N recovery in maize (Zea mays) is partly caused by lack of synchronization between the hedgerow trees N release and the associated food crop N demand. The N not taken up by the associated crop can be immobilized in soil organic matter or assimilated by the hedgerow trees and thus remain in the system. This N can also be lost from the system through denitrification, volatilization or is leached beyond the rooting zone. Below ground contribution (from root turnover and nodule decay) to an associated food crop in alley cropping is estimated at about 25-102 kg N ha-1 season-1. Timing and severity of pruning may allow for some management of underground transfer of fixed N2 to associated crops. However many...

show abstract

“…In a pot experiment with Leucaena leucocephala and Acacia albida at Vienna, Sanginga et al (1990) reported that the %Ndfa of the former plant ranged from 37 to 74, while that of the latter plant ranged from 6 to 37. In a greenhouse experiment, Gliricidia septium grown in pots for 12 weeks derived 45% of its N from N 2 fixation (Sanginga et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Natural abundance of 15N in tropical plants with emphasis on tree legumes

Yoneyama

Muraoka

Murakami³

et al. 1993

Plant Soil

View full text Add to dashboard Cite

Natural abundance of tSN (6 tSN) of leaves harvested from tropical plants in Brazil and Thailand was analyzed. The 8 ~SN values of non-Nz-fixing trees in Brazil were +4.5 -+ 1.9%o, which is lower than those of soil nitrogen (+8.0-+2.2%0). In contrast, mimosa and kudzu had very low 6 ~SN values ( -1 . 4 -+ 0 . 5 % @ The t5 1-~N values of Panicum maximum and leguminous trees, except Leucaena leucocephala, were similar to those of non-N2-fixing trees, suggesting that the contribution of fixed N in these plants is negligible. The 6 15N values of non-N2-fixing trees in Thailand were +4.9 +_ 2.0%0. Leucaena leucocephala, Sesbania grandiflora, Casuarina spp. and Cycas spp. had low 6 tSN values, close to the value of atmospheric N 2 (0%e), pointing to a major contribution of N 2 fixation in these plants. Cassia spp. and Tamarindus indica had high 6 15N values, which confirms that these species are non-nodulating legumes. The 6 ~SN values of Acacia spp. and Gliricidia sepium and other potentially nodulating tree legumes were, on average, slightly lower than those of non-N2-fixing trees, indicating a small contribution of N 2 fixation in these legumes.

show abstract

Variation in nodulation and N2 fixation by the Gliricidia sepium/Rhizobium spp. symbiosis in a calcareous soil

Cited by 23 publications

References 9 publications

Diversity and effectiveness of rhizobial strains from Gliricidia sepium native to Reunion Island, Kenya and New Caledonia

Diversity and effectiveness of rhizobial strains from Gliricidia sepium native to Reunion Island, Kenya and New Caledonia

Management of biological N2 fixation in alley cropping systems: Estimation and contribution to N balance

Natural abundance of 15N in tropical plants with emphasis on tree legumes

Contact Info

Product

Resources

About