Variation in the intracellular polypeptide profiles from different isolates of bovine virus diarrhoea virus

Abstract

Variation of the intracellular polypeptides induced in calf testis cells by 5 cloned isolates of bovine virus diarrhoea virus (BVDV) was examined. Three of the isolates were cytopathic (NADL, C 2415 and Pe 515 c) and two were non-cytopathic (C 1226 and Pe 515 nc) in these cells. The isolates Pe 515 c and Pe 515 nc were both isolated from an animal with clinical signs of mucosal disease. In cells infected with NADL, 8 virus specific proteins (vp 1 to vp 8) with molecular weights ranging from 120,000 (vp 1) to 23,000 (vp 8) were detected. Isolates C 2415 and Pe 515 c gave a similar array of polypeptides to NADL, but the 3 cytopathic isolates could be distinguished by the variation in the molecular weights of some of the proteins. The non-cytopathic isolates could also be distinguished from each other by this type of molecular variation; however, one feature that characterised these strains, when compared to the cytopathic isolates, was the absence of vp 2. Comparison of the polypeptides induced by Pe 515 c and Pe 515 nc showed that apart from the lack of vp 2 in the Pe 515 nc virus profile, the molecular weights of the other viral proteins were similar. This supports serological evidence that for mucosal disease to occur the pair of cytopathic and non-cytopathic viruses must be closely related. Four of the polypeptides induced by Pe 515 c were shown to be glycoproteins.

Cytopathic and Non-cytopathic Biotypes of Border Disease Virus Induce Polypeptides of Different Molecular Weight with Common Antigenic Determinants

“…they fail to induce synthesis of a polypeptide comparable in M r to the cytopathic 80K protein (Pocock et al, 1987;Donis & Dubovi, 1987c). They do, however, induce synthesis of a polypeptide of approximately 120K comparable in size to the higher Mr CP virus protein.…”

Recent Advances in Pestivirus Research

“…The fact that pairs of ncp and cp BVDV isolates from MD-affected animals are serologically indistinguishable (but distinct from other pairs) suggests that only minor alterations have occurred. Comparison of the viral polypeptides of the two BVDV biotypes has revealed a difference: whereas cp BVDV isolates possess both p80 and p125 (among other proteins), ncp viruses display only p125 (Akkina, 1982;Donis & Dubovi, 1987c;Pocock et al, 1987). Earlier peptide mapping data have shown p80 and p125 to be structurally related (Purchio et al, 1984a).…”

Comparisons of the Pestivirus Bovine Viral Diarrhoea Virus with Members of the Flaviviridae

“…In cells infected with cytopathic BVDV, a portion of the p125 is cleaved to yield p54 and p80. This processing event appears to be deficient in cells infected with non-cytopathic BVDV (Donis & Dubovi, 1987;Pocock et al, 1987).…”