Variation in the polyphenol composition, antioxidant, and anticancer activity among different Amaranthus species

House, Nivedya Cheerakuzhy; Puthenparampil, Drisya; Malayil, Dhilna; Narayanankutty, Arunaksharan

doi:10.1016/j.sajb.2020.09.026

Cited by 45 publications

(23 citation statements)

References 30 publications

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“…LCMS was conducted in gradient elution mode using a C18 column having the size of 4.6 × 15.0 cm and inner diameter of 5 μm; the 100% methanol (A) and formic acid in water (0.1%, v/v) had been used as the mobile phase at gradient elution mode. The HPLC elution ranges and MS setup were conducted as described in our previous publication by House et al (2020) .…”

Section: Methodsmentioning

confidence: 99%

Phytochemical profiling of Azima tetracantha Lam. leaf methanol extract and elucidation of its potential as a chain-breaking antioxidant, anti-inflammatory and anti-proliferative agent

Malayil

Jose²,

Narayanankutty³

et al. 2021

Saudi Journal of Biological Sciences

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Azima tetracantha, a traditional medicinal plant included in the order Brassicales and family Salvadoraceae, is widely used as a dietary supplement in folklore medicines. The plant is also used for the treatment of rheumatism, diarrhea and other inflammatory disorders. The present investigation focused on the phytochemical composition, radical scavenging, reducing potential and anti-proliferative activities of the A. tetracantha leaves. Quantitative estimation of the polyphenols and flavonoids revealed significantly elevated levels in the methanol extract. Corroborating with this, methanol extract exhibited higher in vitro anti-radical scavenging effect against 2,2-diphenyl-1- picrylhydrazyl (34.14 ± 2.19 μg/mL), and hydrogen peroxide (44.96 ± 1.77 μg/mL), as well as ferric reducing properties (58.24 ± 6.98 μg/mL). The methanolic extract also showed strong lipoxygenase (71.42 ± 6.36 μg/mL) and nitric oxide inhibitory activities (94.23 ± 8.11 μg/mL). Cytotoxic activity against MCF7 cells was found to be higher (IC50= 37.62 ± 2.94 μg/mL), than that of MDAMB231 cells (IC50= 69.11 ± 5.02 μg/mL). The qPCR-based analysis indicated dose-dependent increase in the expression of the pro-apoptotic genes such as executioner caspases and apoptotic protease activating factor-1. Overall, the results indicated the possible use of methanol extract of A. tetracantha leaves as a chain-breaking antioxidant molecule and are capable of inhibiting inflammatory enzymes and the proliferative potential of breast cancer cells.

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Section: Methodsmentioning

confidence: 99%

Phytochemical profiling of Azima tetracantha Lam. leaf methanol extract and elucidation of its potential as a chain-breaking antioxidant, anti-inflammatory and anti-proliferative agent

Malayil

Jose²,

Narayanankutty³

et al. 2021

Saudi Journal of Biological Sciences

Self Cite

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“…Among others, plant-based foods reduce the risk of diabetes, cancer, and cardiovascular diseases [ 3 , 4 ] because, in addition to micro- and macronutrients, they contain many bioactive components such as flavanols, carotenoids, and anthocyanins which have been shown to have health-promoting properties. Phenolic compounds are the most abundant secondary metabolites in plants, accumulating in all tissues and organs, and are at the center of research due to their antioxidant, anti-hypertensive, anti-inflammatory, and anti-carcinogenic effects [ 5 , 6 ]. The increasing awareness of society on the subject has rapidly increased the market demand for fresh products rich in nutritional value and functional properties.…”

Section: Introductionmentioning

confidence: 99%

The Combination of Mild Salinity Conditions and Exogenously Applied Phenolics Modulates Functional Traits in Lettuce

Zhang

Martinelli

Senizza

et al. 2021

Plants

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The quest for sustainable strategies aimed at increasing the bioactive properties of plant-based foods has grown quickly. In this work, we investigated the impact of exogenously applied phenolics, i.e., chlorogenic acid (CGA), hesperidin (HES), and their combinations (HES + CGA), on Lactuca sativa L. grown under normal- and mild-salinity conditions. To this aim, the phenolic profile, antioxidant properties, and enzyme inhibitory activity were determined. The untargeted metabolomics profiling revealed that lettuce treated with CGA under non-stressed conditions exhibited the highest total phenolic content (35.98 mg Eq./g). Lettuce samples grown under salt stress showed lower phenolic contents, except for lettuce treated with HES or HES + CGA, when comparing the same treatment between the two conditions. Furthermore, the antioxidant capacity was investigated through DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,20-azinobis-(3-ethylbenzothiazoline-6-sulfonate)), and FRAP (ferric reducing antioxidant power) assays, coupled with metal-chelating activity and phosphomolybdenum capacity. An exciting increase in radical scavenging capacity was observed in lettuce treated with exogenous phenolics, in both stress and non-stress conditions. The inhibitory activity of the samples was evaluated against target health-related enzymes, namely cholinesterases (acetylcholinesterase; AChE; butyryl cholinesterase; BChE), tyrosinase, α-amylase, and α-glucosidase. Lettuce treated with HES + CGA under non-stress conditions exhibited the strongest inhibition against AChE and BChE, while the same treatment under salinity conditions resulted in the highest inhibition capacity against α-amylase. Additionally, CGA under non-stress conditions exhibited the best inhibitory effect against tyrosinase. All the functional traits investigated were significantly modulated by exogenous phenolics, salinity, and their combination. In more detail, flavonoids, lignans, and stilbenes were the most affected phenolics, whereas glycosidase enzymes and tyrosinase activity were the most affected among enzyme assays. In conclusion, the exogenous application of phenolics to lettuce represents an effective and green strategy to effectively modulate the phenolic profile, antioxidant activity, and enzyme inhibitory effects in lettuce, deserving future application to produce functional plant-based foods in a sustainable way.

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“…The study also observed higher levels of phenolic contents in A. spinosus and high flavonoid content in A. dubius, corroborating to their high antioxidant potential. Among all, A. spinosus and A. dubius also showed better anti-inflammatory and antiproliferative activities [72]. It is well-known that phytoconstituents type and concentration may be influenced by geographical, environmental, and genetic factors, as well as other physiological differences [73].…”

Section: Chemical-based Assaysmentioning

confidence: 98%

A Review of Recent Studies on the Antioxidant Activities of a Third-Millennium Food: Amaranthus spp.

2020

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Amaranth (Amaranthus spp.) plant commonly refers to the sustainable food crop for the 21st century. The crop has witnessed significant attention in recent years due to its high nutritional value and agronomic advantages. It is a relatively well-balanced cosmopolitan food that is a protector against chronic diseases. Usually, the antioxidant activities of amaranth are held responsible for its defensive behavior. Antioxidant activity of plants, generally, is attributed to their phytochemical compounds. The current interest, however, lies in hydrolysates and bioactive peptides because of their numerous biological functions, including antioxidant effect. While the importance of bioactive peptides has been progressively recognized, an integrated review of recent studies on the antioxidant ability of amaranth species, especially their hydrolysates and peptides has not been generated. Hence, in this review, we summarize studies focused on the antioxidant capacity of amaranth renewal over the period 2015–2020. It starts with a background and overall image of the amaranth-related published reviews. The current research focusing on in vitro, in vivo, and chemical assays-based antioxidant activity of different amaranth species are addressed. Finally, the last segment includes the latest studies concerning free radical scavenging activity and metal chelation capacity of amaranth protein hydrolysates and bioactive peptides.

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Variation in the polyphenol composition, antioxidant, and anticancer activity among different Amaranthus species

Cited by 45 publications

References 30 publications

Phytochemical profiling of Azima tetracantha Lam. leaf methanol extract and elucidation of its potential as a chain-breaking antioxidant, anti-inflammatory and anti-proliferative agent

Phytochemical profiling of Azima tetracantha Lam. leaf methanol extract and elucidation of its potential as a chain-breaking antioxidant, anti-inflammatory and anti-proliferative agent

The Combination of Mild Salinity Conditions and Exogenously Applied Phenolics Modulates Functional Traits in Lettuce

A Review of Recent Studies on the Antioxidant Activities of a Third-Millennium Food: Amaranthus spp.

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