Variation of bacteriologic and serologic Salmonella enterica prevalence between cohorts within finishing swine production farms

Rostagno, Marcos H.; Hurd, H. Scott; McKean, James D.

doi:10.1016/j.foodres.2011.01.062

Cited by 10 publications

(7 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The overall prevalence reported in this study is higher than others that have targeted the finishing phase in the US (Wang et al, 2010;Rostagno et al, 2011). Several explanations can be in base such as farm factors, study design, sampling strategy and diagnostic test (Rajic et al, 2005).…”

Section: Discussioncontrasting

confidence: 55%

The use of quantitative Real-Time PCR to estimate Salmonella shed in fecal samples from naturally infected finishing pigs

Pires

Funk

Manuzon

et al. 2011

International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork

View full text Add to dashboard Cite

The objective of this study was to describe the shedding pattern of Salmonella in finishing pigs, as well to quantify the Salmonella load. A longitudinal study was conducted in 12 cohorts of pigs in a multi-site farrow-to-finish production system. At the beginning of each cohort, 50 pigs (10 ± 2 weeks old) were randomly selected and individually identified. Individual pig fecal samples were collected and cultured every 2 weeks for 16 weeks (8 collections). Further, quantitative real-time PCR (q-PCR) targeting the invA gene was performed in a subset of the culture negative samples (555) and in all available culture positive samples. At the time of submission, Salmonella was cultured from 397 (8.74%) of 4540 individual fecal samples. Overall incidence of Salmonella was 24.8% (149/600 pigs). The proportion of positive samples decreased over the finishing period from 16.75% (10 weeks old) to 4.30% (24-26 weeks old). At the present, all Salmonella culture negative samples subjected to q-PCR were PCR negative. Of culture positive samples, 16% were detected by q-PCR, and only 3 % of the samples were within the quantifiable range of detection (>103 colony forming units per gram of feces). Of those samples within the quantifiable range, the bacterial concentration ranged from 1.05x103 to 1.73x106 invA gene copies/g feces. The results suggest that point estimates of Salmonella prevalence may not accurately describe the Salmonella status of finishing pigs. The majority of pigs shed Salmonella at low concentrations. These preliminary data can contribute to quantitative risk assessments of the association between concentrations of Salmonella shed by pigs during the finishing phase and contributions to carcass contamination at slaughter.

show abstract

Section: Discussioncontrasting

confidence: 55%

The use of quantitative Real-Time PCR to estimate Salmonella shed in fecal samples from naturally infected finishing pigs

Pires

Funk

Manuzon

et al. 2011

International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork

View full text Add to dashboard Cite

show abstract

“…Estimates of Salmonella prevalence in finishing pigs in the USA range from 3·4% to 48% [10, 11, 15, 21–26]. The observed proportion of Salmonella -positive samples and cohort prevalence were within the range of these reports.…”

Section: Discussionmentioning

confidence: 72%

“…A limited number have assessed the faecal prevalence over time, with longitudinal studies showing high variability in Salmonella shedding at the farm, cohort and individual animal level [8][9][10][11][12][13][14][15]. Most of these studies have used a cross-sectional study design.…”

Section: Introductionmentioning

confidence: 99%

Longitudinal study of Salmonella shedding in naturally infected finishing pigs

2012

View full text Add to dashboard Cite

A 3-year longitudinal study was conducted on a multi-site farrow-to-finish production system. For each of 18 cohorts at three finishing sites, 50 pigs were randomly selected. Faecal samples were collected every 2 weeks for 16 weeks. Salmonella was cultured from 453 (6·6%) of 6836 faecal samples. The pig-level incidence of Salmonella was 20·8% (187/899 pigs). Salmonella prevalence varied between cohorts and within pigs. The adjusted Salmonella prevalence decreased over the finishing period from 6·4% to 0·8%. Intermittent detection of Salmonella was found in more than 50% of pigs that were positive at more than one collection. The finding that the majority of pigs shed intermittently has implications for surveillance and research study design when determining Salmonella status. The variability in shedding over time, as well as between and within cohorts and pigs suggests that there may be time-variant risk factors for Salmonella shedding in swine.

show abstract

“…Active surveillance samples from chickens from 2007 onward were included, however, the majority of samples were obtained through passive surveillance and the data are therefore biased toward strains causing clinical disease in food-production animals or identified through diagnostic testing for other diseases. Asymptomatic/subclinical infection is common in some animal species and the full diversity of circulating S. Typhimurium at the population level is therefore unlikely to be captured (Rostagno et al, 2012; Troxell et al, 2015). Submissions are also influenced by variation in farmer and veterinarian behavior (Schwaber et al, 2004; Laupland et al, 2007; Rempel and Laupland, 2009).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance Diversity Suggestive of Distinct Salmonella Typhimurium Sources or Selective Pressures in Food-Production Animals

et al. 2019

View full text Add to dashboard Cite

Salmonella enterica subsp. enterica serovar Typhimurium is a common cause of enterocolitis in humans globally, with multidrug resistant (MDR) strains posing an enhanced threat. S. Typhimurium is also a pathogen in food-production animals, and these populations can act as reservoirs of the bacterium. Therefore, surveillance and control measures within food-production animal populations are of importance both to animal and human health and have the potential to be enhanced though improved understanding of the epidemiology of S. Typhimurium within and between food-production animal populations. Here, data from Scotland and national surveillance England and Wales data for isolates from cattle ( n = 1115), chickens ( n = 248) and pigs ( n = 2174) collected between 2003 and 2014 were analyzed. Ecological diversity analyses and rarefaction curves were used to compare the diversity of observed antimicrobial resistance (AMR) profiles between the host species, and within host species populations. Higher AMR profile diversity was observed in isolates from pigs compared to chickens across diversity measures and isolates from cattle for three of four diversity measures. Variation in AMR profile diversity between production sectors was noted, with higher AMR diversity of isolates from broiler compared to layer chickens, breeder compared to rearer and finisher pigs and beef compared to dairy cattle. Findings indicate variation in AMR profile diversity both within and between food-production animal host species. These observations suggest alternate sources of AMR bacteria and/or variation in selective evolutionary pressures within and between food-production animal host species populations.

show abstract

Variation of bacteriologic and serologic Salmonella enterica prevalence between cohorts within finishing swine production farms

Cited by 10 publications

References 39 publications

The use of quantitative Real-Time PCR to estimate Salmonella shed in fecal samples from naturally infected finishing pigs

The use of quantitative Real-Time PCR to estimate Salmonella shed in fecal samples from naturally infected finishing pigs

Longitudinal study of Salmonella shedding in naturally infected finishing pigs

Antimicrobial Resistance Diversity Suggestive of Distinct Salmonella Typhimurium Sources or Selective Pressures in Food-Production Animals

Contact Info

Product

Resources

About