Variation of cell surface hydrophobicity and biofilm formation among genotypes of<i>Candida albicans</i>and<i>Candida dubliniensis</i>under antifungal treatment

Borecká-Melkusová, Silvia; Bujdáková, Helena

doi:10.1139/w08-060

Cited by 34 publications

(23 citation statements)

References 40 publications

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“…Therefore, a combination with microscopy is necessary. As expected, all tested strains were able to form a biofilm, but this ability proved to be strain-dependent for both C. albicans as ell as C. dubliniensis, as was previously described by Borecká-Melkusová et al [29]. It is of interest that while C. dubliniensis was able to form as vital biofilm as C. albicans on a polystyrene surface, SEM microscopy revealed a morphological diversity between both spp.…”

Section: Discussionsupporting

confidence: 81%

Biofilm formation and adhesive/invasive properties of Candida dubliniensis in comparison with Candida albicans

et al. 2011

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Candida dubliniensis and Candida albicans are closely related spp. exhibiting differences in their virulence potency. This study compared clinical isolates of C. dubliniensis with C. albicans from HIV patients with oropharyngeal candidiasis (OPC) and standard strains in power to form biofilm and their adhesive and invasive properties. Members of both spp. were able to form strong biofilms. However, SEM microscopy confirmed that C. albicans undergoes the more effective yeast-to-hyphae transition than C. dubliniensis with prevalent yeast form and limited ability to form filaments. Kinetic patterns indicated that while the first 30 min are critical for sufficient attachment to a polystyrene surface, adhesion to human carcinoma cell lines (Caco-2 and TR 146) needs additional time with maximal saturation observed at 240 min for both spp. The invasion process was tested on 3D RHE (reconstituted human epithelium) with Caco-2 or TR 146 on the collagen surface. C. albicans rapidly produced hyphae that penetrated the tissue layer, demonstrating substantive invasion within 21 h. In contrast, C. dubliniensis attached to the tissue surface and proliferated, suggesting the formation of a biofilm-like structure. After 21 h, C. dubliniensis was able to penetrate the RHE layer and invade unusually, with a cluster of the yeast cells

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Section: Discussionsupporting

confidence: 81%

Biofilm formation and adhesive/invasive properties of Candida dubliniensis in comparison with Candida albicans

et al. 2011

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“…Although the formation of a biofilm in the environment is a natural process important for the survival of many microorganisms, medical microbiology regards this complex structure as a serious complication during patient treatment or convalescence. Current trends in biofilm studies are aimed at possible ways to eliminate them, mainly via the application of antifungal agents (Kuhn et al , 2002; Al‐Fattani & Douglas, 2004; Seidler et al , 2006; Borecká‐Melkusová & Bujdáková, 2008). However, some authors have published different thoughts on biofilm treatment, such as photodynamic effects (Müller et al , 2007; Dovigo et al , 2009) or using antibodies (Rodier et al , 2003; Fujibayashi et al , 2009; Maza et al , 2009).…”

Section: Resultsmentioning

confidence: 99%

Participation of theCandida albicanssurface antigen in adhesion, the first phase of biofilm development

Bujdáková¹,

Paulovičová²,

Paulovičová³

et al. 2010

FEMS Immunol Med Microbiol

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The lack of work dealing with possible ways of reducing biofilm production via inhibiting Candida albicans adherence in the first stage of biofilm formation was a motivation for this study. The study was focused on two questions: (1) can a decrease in adherence affect the quantity of mature biofilm? and (2) can blocking the surface C. albicans complement receptor 3-related protein (CR3-RP) with polyclonal anti-C3-RP antibody or monoclonal antibody OKM1 significantly contribute to a reduction in adherence during biofilm formation? The presence and quantity the CR3-RP expressed in the biofilm was confirmed by immunofluorescence, immunocytometry and enzyme-linked immunosorbent assay. To determine the changes in adherence of C. albicans CCY 29-3-162 and C. albicans catheter isolate, 30-, 60-, 90-and 120-min time points were selected and viability was determined by XTT assay. The strains were preincubated with both antibodies to block CR3-RP, which proved to be effective at reducing adhesion and the formation of a mature biofilm (64.1-74.6%). The duration of adhesion, between 30 and 120 min, seems to have a significant effect on the mature biofilm. The blocking of CR3-PR by antibodies before adherence affected the fitness of biofilm, which was not able to revitalize in the later stages.

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“…Despite the significant phenotypic and genotypic similarities shared between C. albicans and C. dubliniensis , the comparative virulence of the two species is clearly a very complex topic [40,41]. Borecká-Melkusová [42] verified that the biofilm formation in C. albicans was significantly lower than in C. dubliniensis , and Koga-Ito et al [43] observed that the survival rate and dissemination capacity of C. dubliniensis in mice were lower than C. albicans .…”

Section: Discussionmentioning

confidence: 99%

Oral Candida albicans isolates from HIV-positive individuals have similar in vitro biofilm-forming ability and pathogenicity as invasive Candida isolates

et al. 2011

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BackgroundCandida can cause mucocutaneous and/or systemic infections in hospitalized and immunosuppressed patients. Most individuals are colonized by Candida spp. as part of the oral flora and the intestinal tract. We compared oral and systemic isolates for the capacity to form biofilm in an in vitro biofilm model and pathogenicity in the Galleria mellonella infection model. The oral Candida strains were isolated from the HIV patients and included species of C. albicans, C. glabrata, C. tropicalis, C. parapsilosis, C. krusei, C. norvegensis, and C. dubliniensis. The systemic strains were isolated from patients with invasive candidiasis and included species of C. albicans, C. glabrata, C. tropicalis, C. parapsilosis, C. lusitaniae, and C. kefyr. For each of the acquired strains, biofilm formation was evaluated on standardized samples of silicone pads and acrylic resin. We assessed the pathogenicity of the strains by infecting G. mellonella animals with Candida strains and observing survival.ResultsThe biofilm formation and pathogenicity in Galleria was similar between oral and systemic isolates. The quantity of biofilm formed and the virulence in G. mellonella were different for each of the species studied. On silicone pads, C. albicans and C. dubliniensis produced more biofilm (1.12 to 6.61 mg) than the other species (0.25 to 3.66 mg). However, all Candida species produced a similar biofilm on acrylic resin, material used in dental prostheses. C. albicans, C. dubliniensis, C. tropicalis, and C. parapsilosis were the most virulent species in G. mellonella with 100% of mortality, followed by C. lusitaniae (87%), C. novergensis (37%), C. krusei (25%), C. glabrata (20%), and C. kefyr (12%).ConclusionsWe found that on silicone pads as well as in the Galleria model, biofilm formation and virulence depends on the Candida species. Importantly, for C. albicans the pathogenicity of oral Candida isolates was similar to systemic Candida isolates, suggesting that Candida isolates have similar biofilm-forming ability and virulence regardless of the infection site from which it was isolated.

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Variation of cell surface hydrophobicity and biofilm formation among genotypes ofCandida albicansandCandida dubliniensisunder antifungal treatment

Cited by 34 publications

References 40 publications

Biofilm formation and adhesive/invasive properties of Candida dubliniensis in comparison with Candida albicans

Biofilm formation and adhesive/invasive properties of Candida dubliniensis in comparison with Candida albicans

Participation of theCandida albicanssurface antigen in adhesion, the first phase of biofilm development

Oral Candida albicans isolates from HIV-positive individuals have similar in vitro biofilm-forming ability and pathogenicity as invasive Candida isolates

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