Variation of Collagenous and Non-Collagenous Proteins of Human Knee Joint Menisci with Age and Degeneration

Ingman, A.M.; Ghosh, Peter; Taylor, T.K.F.

doi:10.1159/000212017

Cited by 59 publications

(25 citation statements)

References 5 publications

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“…The total collagen content was derived from hydroxyproline by multiplying its value by a factor of 7.4 as suggested by Ingman et al . [27]. The determination of total sulphated GAG content was based on the method described by Ferndale et al .…”

Section: Methodsmentioning

confidence: 99%

Experimental research Evidence for apoptosis, MMP-1, MMP-3 and TIMP-2 expression and their effect on the mechanical and biochemical properties of fresh viable knee medial meniscal allografts and autografts in the rabbit

Zwierzchowski¹,

Stasikowska-Kanicka²,

Janus³

et al. 2012

aoms

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IntroductionThe study sought evidence for apoptosis, the expression of MMP-1, MMP-3 and TIMP-2 and their effect on the mechanical and biochemical properties of rabbit fresh knee medial meniscal grafts in a 6-month follow-up.Material and methodsForty white male New Zealand rabbits were chosen for the study. The medial meniscus was excised from 28 animals and stored under tissue culture conditions for 2 weeks, following which 14 of them were implanted as autografts and 14 as allografts. When the animals were euthanized, 20 menisci were used for immunohistochemical examinations. Apoptosis (TUNEL method) and MMP-1, MMP-3 and TIMP-2 immunoexpression were estimated semiquantitatively. The other 20 menisci were subjected to biochemical analysis and their degree of elasticity was evaluated.ResultsAn increased level of apoptosis (p <0.05) was observed both in allografts (1.57 ±0.98) and autografts (0.86 ±0.69); no statistical differences existed between them. An increased level of metalloproteinases and TIMP-2 expression was observed only in the allografts (p < 0.05). The highest decrease of degree of elasticity and the most significant changes in biochemical composition were observed in allografts (p < 0.05).ConclusionsThe studies confirmed the existence of excessive apoptosis in both kinds of fresh viable medial meniscal implants: auto- and allografts. Our results suggest that apoptosis and increased MMP-1 and MMP-3 expression have an adverse effect on the biological properties of implants. The results of experimental studies on humans indicate the need to devise a method of apoptosis inhibition in transplanted menisci to improve long-term results.

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Section: Methodsmentioning

confidence: 99%

Experimental research Evidence for apoptosis, MMP-1, MMP-3 and TIMP-2 expression and their effect on the mechanical and biochemical properties of fresh viable knee medial meniscal allografts and autografts in the rabbit

Zwierzchowski¹,

Stasikowska-Kanicka²,

Janus³

et al. 2012

aoms

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“…58 The noncollagenous matrix proteins showed the most profound changes, decreasing from 21.9% ± 1.0% (dry weight) in neonates to 8.1% ± 0.8% between the ages of 30 to 70 years. 80 After 70 years of age, the noncollagenous matrix protein levels increased to 11.6% ± 1.3%. Peters and Smillie observed an increase in hexosamine and uronic acid with age.…”

Section: Maturation and Aging Of The Meniscusmentioning

confidence: 96%

The Basic Science of Human Knee Menisci

Fox

Bedi

Rodeo

2011

Sports Health: A Multidisciplinary Approach

478

263

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Context:Information regarding the structure, composition, and function of the knee menisci has been scattered across multiple sources and fields. This review contains a concise, detailed description of the knee menisci—including anatomy, etymology, phylogeny, ultrastructure and biochemistry, vascular anatomy and neuroanatomy, biomechanical function, maturation and aging, and imaging modalities.Evidence Acquisition:A literature search was performed by a review of PubMed and OVID articles published from 1858 to 2011.Results:This study highlights the structural, compositional, and functional characteristics of the menisci, which may be relevant to clinical presentations, diagnosis, and surgical repairs.Conclusions:An understanding of the normal anatomy and biomechanics of the menisci is a necessary prerequisite to understanding the pathogenesis of disorders involving the knee.

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“…As a consequence, GAG content is high in the medial 2/3. 9 Entire width of the reformed menisci was stained diffusely in light red which also suggested that the entire width of the reformed menisci received tension force equally by altered biomechanics and different histology. Different shape and histology of the reformed menisci and different GAG distribution and contents may explain the inferiority of the reformed menisci.…”

Section: Discussionmentioning

confidence: 96%

Glycosaminoglycan Changes in Regenerative Menisci of the Immature Rabbits — Histological and Spectrophotometric Quantitation

Moon

Kim

Chu

et al. 2014

J. Musculoskelet. Res.

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Purpose: This study was conducted primarily to compare the contents and distribution of Glycosaminoglycan (GAG) in the normal and regenerated menisci, and partly to elucidate the role of GAG in the meniscus to fulfill its function. Method: A total of 60 immature rabbits, weighing 300-400 gm and aged 4 weeks, were used for the study. Total medial meniscectomy in the right knee joint was performed in all rabbits. The medial menisci of the left knee joints were used as control. After 16, 20, 24 and 28 weeks postoperatively, the amount of GAG in normal and regenerated menisci were measured. For the measurement of quantity and distribution of GAG contents, photon absorptiometry and Safranin O staining method were used. Results: (1) In 48 of the 60 medial meniscectomized knees, menisci were regenerated in various sizes with degenerative changes of joints, especially on tibial side.(2) In normal menisci, GAG was distributed at inner and middle 1/3, while GAG in regenerated menisci was diffusely found in the entire width. (3) The concentration of GAG per 1 mg of regenerated menisci were 4:36 AE 1:68, 4:58 AE 1:14, 4:65 AE 2:21, 4:60 AE . Downloaded from www.worldscientific.com by UNIVERSITY OF AUCKLAND LIBRARY -SERIALS UNIT on 03/22/15. For personal use only.24, and 28 weeks groups during the observation periods ( p > 0:05). 5. There was a distinctive difference in Safranin O stainability by every 4 g increase of GAG in both 1 mg of normal and regenerated menisci. Conclusion: This study showed the quantitative change of GAG between normal and regenerated menisci, and comparative analysis of GAG contents in the menisci by biochemical method and histological method were found to be useful tools.

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Variation of Collagenous and Non-Collagenous Proteins of Human Knee Joint Menisci with Age and Degeneration

Cited by 59 publications

References 5 publications

Experimental research Evidence for apoptosis, MMP-1, MMP-3 and TIMP-2 expression and their effect on the mechanical and biochemical properties of fresh viable knee medial meniscal allografts and autografts in the rabbit

Experimental research Evidence for apoptosis, MMP-1, MMP-3 and TIMP-2 expression and their effect on the mechanical and biochemical properties of fresh viable knee medial meniscal allografts and autografts in the rabbit

The Basic Science of Human Knee Menisci

Glycosaminoglycan Changes in Regenerative Menisci of the Immature Rabbits — Histological and Spectrophotometric Quantitation

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