Variation of protein kinase C-α expression in eyestalk removal-activated ovaries in whiteleg shrimp, Litopenaeus vannamei

Chen, Hsiang-Yin; Kang, Bong Jung; Sultana, Zakea; Wilder, Marcy N.

doi:10.1016/j.cbpa.2019.110552

Cited by 4 publications

(4 citation statements)

References 16 publications

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“…Total RNAs extracted from the incubated ovarian explants with RNeasy Mini Kit (250) (Qiagen, Hilden, Germany) were used as templates. Vg and β-actin were amplifed by quantitative real-time PCR using the ABI PRISM 7500 sequence detection system v2.3 (Applied Biosystem, Foster City, California, USA), as given in a previous report [17]. Sequences of primers and probes used in amplifcation are given in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Examination of the Effects of Thoracic Ganglion and Brain Extracts on Vitellogenin Gene Expression in the Ovary of Whiteleg Shrimp, Litopenaeus vannamei, and Possible Vitellogenesis-Stimulating Activity

Sultana

Kang

Wilder

2023

Aquaculture Research

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In order to examine the role of the central nervous system in regulating ovarian maturation in the whiteleg shrimp, Litopenaeus vannamei, we incubated ovarian explants taken from adult shrimp with either brain or thoracic ganglion extracts at the level of five tissue equivalents for 6 h. For assessment of the effects of each tissue extract, transcriptional expression of vitellogenin mRNA in incubated ovarian tissue fragments was analyzed by quantitative real-time PCR. Results showed that crude brain extracts and a 90% aqueous acetonitrile eluate upregulated expression levels in a significant manner. These findings suggest that factors originating from brain tissue are involved in ovarian maturation, but that the thoracic ganglia do not likely harbor significant quantities of stimulatory substances. In the future, this knowledge may be useful in developing technology for inducing maturation and spawning in captive female penaeid.

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Section: Methodsmentioning

confidence: 99%

Examination of the Effects of Thoracic Ganglion and Brain Extracts on Vitellogenin Gene Expression in the Ovary of Whiteleg Shrimp, Litopenaeus vannamei, and Possible Vitellogenesis-Stimulating Activity

Sultana

Kang

Wilder

2023

Aquaculture Research

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“…In L. vannamei , Alnawafleh et al ( 32 ) provided evidence of the involvement of a Ca 2+ /PKC signaling cascade in vitellogenesis. On the contrary, Chen et al ( 150 ) reported that PKC-α isoform may regulate ovarian growth in L. vannamei through a negative-based regulating mechanism. The functioning of Ca 2+ signaling in ovarian development reported in different species is inconsistent, with strong evidence for fundamentally opposite (inducing and hindering) functions being attributed to it ( 15 , 143 , 148 , 150 , 151 ).…”

Section: Methyl Farnesoate (Mf)mentioning

confidence: 97%

Insights on Molecular Mechanisms of Ovarian Development in Decapod Crustacea: Focus on Vitellogenesis-Stimulating Factors and Pathways

2020

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Vitellogenesis in crustaceans is an energy-consuming process. Though the underlying mechanisms of ovarian maturation in decapod Crustacea are still unclear, evidence indicates the process to be regulated by antagonistically-acting inhibitory and stimulating factors specifically originating from X-organ/sinus gland (XO/SG) complex. Among the reported neuromediators, neuropeptides belonging to the crustacean hyperglycemic hormone (CHH)-family have been studied extensively. The structure and dynamics of inhibitory action of vitellogenesis-inhibiting hormone (VIH) on vitellogenesis have been demonstrated in several species. Similarly, the stimulatory effects of other neuropeptides of the CHH-family on crustacean vitellogenesis have also been validated. Advancement in transcriptomic sequencing and comparative genome analysis has led to the discovery of a large number of neuromediators, peptides, and putative peptide receptors having pleiotropic and novel functions in decapod reproduction. Furthermore, differing research strategies have indicated that neurotransmitters and steroid hormones play an integrative role by stimulating neuropeptide secretion, thus demonstrating the complex intertwining of regulatory factors in reproduction. However, the molecular mechanisms by which the combinatorial effect of eyestalk hormones, neuromediators and other factors coordinate to regulate ovarian maturation remain elusive. These multifunctional substances are speculated to control ovarian maturation possibly via the autocrine/paracrine pathway by acting directly on the gonads or by indirectly exerting their stimulatory effects by triggering the release of a putative gonad stimulating factor from the thoracic ganglion. Acting through receptors, they possibly affect levels of cyclic nucleotides (cAMP and cGMP) and Ca 2+ in target tissues leading to the regulation of vitellogenesis. The “stimulatory paradox” effect of eyestalk ablation on ovarian maturation continues to be exploited in commercial aquaculture operations, and is outweighed by the detrimental physiological effects of this procedure. In this regard, the development of efficient alternatives to eyestalk ablation based on scientific knowledge is a necessity. In this article, we focus principally on the signaling pathways of positive neuromediators and other factors regulating crustacean reproduction, providing an overview of their proposed receptor-mediated stimulatory mechanisms, intracellular signaling, and probable interaction with other hormonal signals. Finally, we provide insight into future research directions on crustacean reproduction as well as potential applications of such research to aquaculture technology development.

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“…In female crustacean species, the accumulation of the major yolk protein, vitellin, within the oocytes defines ovarian maturation (Tsukimura, 2001). Vitellogenin (Vtg) is first synthesized in the hepatopancreas and ovaries and is the precursor of vitellin (Chen et al, 2019; Quackenbush, 1992). Vitellogenin is processed into smaller molecules and then accumulated in the developing oocytes as vitellin (Warrier & Subramoniam, 2002).…”

Section: Introductionmentioning

confidence: 99%

Redclaw Crayfish Cherax quadricarinatus LRPPRC : The characterization of a sex‐linked gene and its relationship with vitellogenin

Lin

Guo

Zhao

et al. 2022

Aquaculture Research

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In the Redclaw Crayfish, Cherax quadricarinatus, males grow larger and faster than females, and interest has focused on monosex breeding techniques for the production of males only. The adult male and female sexual characteristics of Cherax quadricarinatus are easily distinguished but cannot be determined in the embryo and juvenile stages. Because there is no DNA marker for the identification of sex in Cherax quadricarinatus, we sequenced its juvenile transcriptome in order to produce a reliable transcript to determine sex and to investigate its sex differentiation mechanisms at the molecular level. In this study, a female sex‐linked expression gene CqLRPPRC (Leucine‐rich PPR‐motif‐containing) of Cherax quadricarinatus was found in the transcriptome. Using semi‐quantitative RT‐PCR analysis, we found that the expression of the LRPPRC (leucine‐rich pentatricopeptide repeat cassette) gene was greater in the ovaries of females than in the testes of males, from the early juvenile stages onwards. Furthermore, in situ hybridization showed that the expression of its sexual dimorphism may be focused in the gonads. The results of an RNA interference treatment showed that a knockdown of CqLRPPRC up‐regulated the expression of vitellogenin (Vtg). The LRPPRC gene, and characteristic of shrimp, are reported here for the first time, and an assay of this gene can provide a molecular test to determine the sex of Cherax quadricarinatus in its different developmental stages, especially those during which no obvious sexual differences are visible.

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Variation of protein kinase C-α expression in eyestalk removal-activated ovaries in whiteleg shrimp, Litopenaeus vannamei

Cited by 4 publications

References 16 publications

Examination of the Effects of Thoracic Ganglion and Brain Extracts on Vitellogenin Gene Expression in the Ovary of Whiteleg Shrimp, Litopenaeus vannamei, and Possible Vitellogenesis-Stimulating Activity

Examination of the Effects of Thoracic Ganglion and Brain Extracts on Vitellogenin Gene Expression in the Ovary of Whiteleg Shrimp, Litopenaeus vannamei, and Possible Vitellogenesis-Stimulating Activity

Insights on Molecular Mechanisms of Ovarian Development in Decapod Crustacea: Focus on Vitellogenesis-Stimulating Factors and Pathways

Redclaw Crayfish Cherax quadricarinatus LRPPRC : The characterization of a sex‐linked gene and its relationship with vitellogenin

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