Variation of serum iron concentration in young healthy men: within-day and day-to-day changes

Statland, Bernard E.; Winkel, Per; Bokelund, H.

doi:10.1016/s0009-9120(76)80008-2

Cited by 67 publications

(21 citation statements)

References 5 publications

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“…Friedel et al (3), however, demonstrated, that beside contamination with interstitial fluid and damaged tissue, obstruction of venous back flow caused the nonfiltrable plasma elements (e. g. protein) to become more concentrated owing to increased hydrostatic pressure. This phenomenon also holds true for tourniquet application in man (53). The "criticar enzymes especially are falsely elevated in plexus blood from guinea pigs (47) and rats (3, 47, 54-56).…”

Section: Blood Drawing Techniquesmentioning

confidence: 86%

Catalytic Enzyme Activity Concentration in Plasma of Man, Sheep, Dog, Cat, Rabbit, Guinea Pig, Rat and Mouse. Approach to a Quantitative Diagnostic Enzymology. I. Communication

Lindena¹,

Trautschold²

1986

Clinical Chemistry and Laboratory Medicine

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Summary:For the most convenient laboratory animals in experimental medicine such äs the sheep, dog, cat, rabbit, guinea pig, rat and mouse, the catalytic concentrations of seventeen enzymes and the protein and albumin concentrations in plasma were determined. The corresponding data for man were taken from the literature. The experimental conditions were selected so äs to minimize the influence on catalytic activity, variance and statistical distribution pattern of such factors äs handling procedures, the choice of blood drawing technique, the choice of anaesthetic agent for distinct species and the preparation procedure of plasma from the blood specimen. Because these considerations have been largely neglected to date in experimental enzymology, normal values for most enzymes require revision and were in some instances established for the first time in certain species. Our use of assay conditions, which were identical for all animal species, satisfies current requirements in clinical chemistry with respect to standardization and optimization of enzyme catalytic activity concentration measurement, and allowed comparison of these enzymes. The catalytic activities established this way will be the basis for a later comparative study of their kinetics of adjustment in plasma. Katalytische Konzentration von Enzymen im Plasma von Mensch und Versuchstieren Versuch der Begründung einer quantitativen Diagnostisch-!· Enzymologie, I. Zusammenfassung: Bei den in der experimentellen

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Section: Blood Drawing Techniquesmentioning

confidence: 86%

Catalytic Enzyme Activity Concentration in Plasma of Man, Sheep, Dog, Cat, Rabbit, Guinea Pig, Rat and Mouse. Approach to a Quantitative Diagnostic Enzymology. I. Communication

Lindena¹,

Trautschold²

1986

Clinical Chemistry and Laboratory Medicine

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“…Additionally, combinations of iron(II) and ascorbic acid concentrations were used: 18 µmol/L iron(II) with 60 µmol/L ascorbic acid (low iron/ ascorbate) and 590 µmol/L iron(II) with 1.2 mmol/L ascorbic acid (high iron/ascorbate). The low iron and ascorbic acid concentrations resemble human plasma (Statland et al 1976;Simon and Hudes 1999) and the higher concentrations were chosen according to the peptide/iron(II)/ascorbic acid ratio shown most effectively oxidizing peptides (Bollineni et al 2011). The Amadori peptide degradation was always completed within 30 min regardless of the reaction conditions, similar to the buffer condition described above.…”

Section: Kinetics Of Amadori Degradation and Product Formationmentioning

confidence: 99%

Oxidative degradation of N ε-fructosylamine-substituted peptides in heated aqueous systems

2015

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Glycation, or non-enzymatic glycosylation, is a common protein modification formed by reactions between reducing sugars (i.e. aldoses and ketoses) with protein amino groups. Resulting Amadori and Heyns compounds, respectively, can be oxidatively degraded yielding a structurally heterogeneous group of advanced glycation end-products. We have studied this process in aqueous conditions at 95 °C in terms of appearing products and their formation kinetics in the presence or absence of reactive oxygen species (ROS)-generating systems (iron(II) sulfate). RP-HPLC-ESI-MS revealed 20 products, 12 of which were confirmed after synthesis by identical retention times and fragmentation patterns. These products accumulated during the incubation period of 4 h (N(ε)-carboxymethyl-, N(ε)-formyl- and N(ε)-methyl lysine) or appeared intermediately (2-aminoadipic semialdehyde, N(ε)-ethanalyl lysine). Acidic and basic amino acid residues near the glycation site and elevated ROS levels in the reaction mixture had significant effects on both product formation and degradation kinetics.

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“…Friedel et al (15), however, demonstrated that beside contamination with interstitial fluid and damaged tissue, obstruction of venous back flow caused the nonfiltrable plasma elements (e.g. proteins) to become more concentrated owing to the increased hydrostatic pressure, a phenomenon which also holds true for tourniquet application in humans (8). In fact, especially the catalytic activities of the "critical enzymes" determined in plasma from retroorbital plexus are much to high (17)(18)(19).…”

Section: Discussionmentioning

confidence: 99%

Biological, Analytical and Experimental Components of Variance in a Long-Term Study of Plasma Constituents in Rat

Lindena¹,

Büttner²,

Trautschold³

1984

Clinical Chemistry and Laboratory Medicine

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Summary: Statistical analysis of variance was applied to data from determinations of 14 plasma constituents in 25 rats in order to evaluate the analytical* experimental and biological (inter-and intraindividual) component of variance. Blood was taken seven times in intervals of 8-10 days, the last one by catheter technique and the other by heart puncture. The analytical portion of variance was determined by the concurrent analysis of a pool plasma Standard. The experimental component of variance was evaluated by the comparison of the Variation of the catheter values with that of the pooled data froin heart puncture. The coefficient of Variation for the latter may be grouped into three categories: less than 10% for protein, Na + , K + , Ca 2+ ; 10-20% for urea, phosphate and the enzymes äs alanine aminotransferase, choline esterase, alkaline phosphatase and leucine arylamidase and 20^-65% for the other enzymes lactate dehydrogenase, malate dehydrogenase, aspartate aminotransferase and creatine kinase. The results from the samples taken by catheter technique generälly revealed the lower values for the mean äs well äs for the variance. It became evident that the procedure of heart puncture is afflicted with the most aggravating interference factors, thus accounting for most of the experimental component of variance. The observed differences between the single blood drawings, the nonGaussian distribution for several constituents, and the interactions between the components of variance do not always fit for the Statistical concept of additivity of the single components. Therefore the result for the partition öf the variance in single components may be more or less approximative in the strict sense of Statistical values, but it provides a practical guide for clinical chemical research with small laboratory animals. Biologische, analytische und experimentelle Varianzkomponenten von Plasmamerkmalen in einer Langzeitstu

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Variation of serum iron concentration in young healthy men: within-day and day-to-day changes

Cited by 67 publications

References 5 publications

Catalytic Enzyme Activity Concentration in Plasma of Man, Sheep, Dog, Cat, Rabbit, Guinea Pig, Rat and Mouse. Approach to a Quantitative Diagnostic Enzymology. I. Communication

Catalytic Enzyme Activity Concentration in Plasma of Man, Sheep, Dog, Cat, Rabbit, Guinea Pig, Rat and Mouse. Approach to a Quantitative Diagnostic Enzymology. I. Communication

Oxidative degradation of N ε-fructosylamine-substituted peptides in heated aqueous systems

Biological, Analytical and Experimental Components of Variance in a Long-Term Study of Plasma Constituents in Rat

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