Variations de l'activité α-amylasique pariétale et intraluminale dans le tube digestif de lapins témoins et à canal pancréatique ligaturé

Abstract

Summary. a-amylase activity variations in the parietal and intraluminal contents of the digestive tract in control and pancreatic duct ligated rabbits. a-amylase activity (E.C.3.2.1 .1) was studied in the parietal and intraluminal contents of different parts of the digestive tract in control (T) and pancreatic duct ligated rabbits (P-). Maximal activity at the parietal level in control rabbits was observed in the median part of the duodenum (392 ! 37 fL M/maltose/g/min at 37 °C). Weak activity (3 p. 100 of the duodenal activity) was seen in other parts of the small and large bowel. Variations in a-amylase activity were noted in the post-ligature period in rabbits. This activity declined by 38 p. 100 2 weeks after ligature ; normal values reappeared 6 weeks later, and 10 weeks after the operation the values were 40 p. 100 higher than those of control rabbits. Maximal intraluminal content activity was observed in the jejunum and ileum of T rabbits (182 !26 and 92 ! 6 y m jmaltosejg jmin at 37 °C).The large bowel showed only 5 p. 100 of the jejunal activity. In P-rabbits, a-amylase activity progressively increased only in the large bowel from 2 and 6 weeks ( x 3) to 10 weeks ( x 6). Suppression of pancreatic digestion was followed by a compensative process. Hyperplasia and hypertrophy of Briinner's glands could explain the rise in a-amylase activity at the duodenal level. The elevation observed in intraluminal contents was certainly of bacterial origin.Il est connu que chez des animaux qui présentent des troubles pancréatiques, il apparaît souvent une variation des activités enzymatiques intestinales. Plus précisé-ment, dans le cas de déficience pancréatique exocrine, certains auteurs ont pu constater une élévation des hydrolases glucidiques, principalement des disaccharidases (Cerda, Preiser et Crane, 1972 ;Madzarovova-Nohejlova, 1976

Insulin and Glucagon Impairments in Relation with Islet Cells Morphological Modifications Following Long Term Pancreatic Duct Ligation in the Rabbit – A Model of Non‐insulin‐dependent Diabete

“…(Magnification 192). than that found in previous data [111 with a maximum (15.1 + 1.2mmol/1) that correlated (r= -0.94, p < 0.001) with the nadir in insulin levels (42 +__ 8pmol/1) on day 30 (Fig. lb).…”

Adaptation of the Activities of the Brush Border Hydrolases along the Small Intestine of the Rabbit after Pancreatic Duct Ligation

“…When they are situated at the external face of the mem brane, the degradation could be chiefly acti vated by bacterial proteases who can hydro lyse brush border enzymes [1,2], In the rab bit, it is of a great interest to observe a prolif eration of microorganisms after pancreatic duct ligation [13]: bacterial proteases [1] might play a major role in the extracellular degradation processes. These bacteria are even able to synthetize an intraluminal alpha amylase at the level of the large intestine after pancreatic duct ligation [12].…”