Variations in Expression of Cell Membrane Antigens by Cultured Cells

Rosenfeld, C.; Jf, Doré; Choquet, C.; Am, Venuat; Ajuria, E; Marholev, L; Jp, Wastiaux

doi:10.1097/00007890-197310000-00002

Cited by 10 publications

(4 citation statements)

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“…A double block by a thymidine excess has long been used to synchronize cells, and it has long been known that such a synchronization results from a diminution of the progression of cells through the S phase of the cell cycle . The concentrations of thymidine required to achieve cell synchronization, however, were usually 20-50 times higher (Rosenfeld et al, 1973) than the concentrations of thymidine dinucleotide used in the experiments reported here that resulted in S phase accumulation of treated melanoma cells. Indeed, exposure of the melanoma cells to comparable amounts of thymidine mononucleotide (100 µM) failed to result in significant cell cycle changes, although further increasing thymidine mononucleotide concentration up to 300 µM resulted in S phase arrest.…”

Section: Discussionmentioning

confidence: 71%

Thymidine Dinucleotides Induce S Phase Cell Cycle Arrest in Addition to Increased Melanogenesis in Human Melanocytes

Pedeux

Al-Irani

Marteau

et al. 1998

Journal of Investigative Dermatology

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Although the induction of pigmentation following exposure of melanocytes to ultraviolet light in vivo and in vitro is well documented, the intracellular mechanisms involved in this response are not yet fully understood. Exposure to UV-B radiation leads to the production of DNA damage, mainly cyclobutane pyrimidine dimers, and it was recently suggested that the thymidine dinucleotide pTpT, mimicking small DNA fragments released in the course of excision repair mechanisms, could trigger melanin synthesis. We now report that the thymidine dinucleotide pTpT induces melanogenesis both in human normal adult melanocytes and in human melanoma cells. Thus, the SOS-like response suggested by Gilchrest's work to be evolutionary conserved, based primarily on work in murine cells and guinea pigs, is also apparently present in the human. Thymidine dinucleotide is nontoxic to melanoma cells and does not induce apoptosis in these cells, but induces S phase cell cycle arrest and a proliferation slow down. Because thymidine excess in culture medium leads to the synchronization of cells in S phase, we investigated whether this phenomenon was involved in the increase in melanin synthesis. We show that melanin synthesis is specifically triggered by the dimeric form of the thymidine and not by the monomeric form pT. Thus, our data strongly support that thymidine dinucleotides pTpT mimic at least part of the effects of ultraviolet irradiation, and may hence represent an invaluable model in the study of the molecular events involved in melanogenesis induction triggered through DNA damage.

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Section: Discussionmentioning

confidence: 71%

Thymidine Dinucleotides Induce S Phase Cell Cycle Arrest in Addition to Increased Melanogenesis in Human Melanocytes

Pedeux

Al-Irani

Marteau

et al. 1998

Journal of Investigative Dermatology

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“…Species-specific antigens would be represented on both blast and remission leukocytes but might influence the activity of the unabsorbed antisera since the distribution of these antigens amongst blast cells and remission cells is unknown. In the present study, neoantigens appearing by virtue of cell culture techniques (17)(18)(19) are not a factor as they may be in other heteroantisera studies (9).…”

Section: Introductionmentioning

confidence: 56%

“…The use of cells from a single patient avoids many of the problems of using pooled or third party cells, such as alloantigenic differences or antigens acquired in tissue culture (17)(18)(19)). …”

Section: Introductionmentioning

confidence: 99%

“…The reappearance of antigens characteristic of the fetus is known to occur in some human malignancies, and there is evidence that this may occur in leukemia (40). Cell membrane characteristics reflecting the metabolic changes of the phases of the replicative cell cycle may include changes in cell-surface antigenicity (18,19,4143). Leukemic blast cells by virtue of dominance of certain cell-cycle phases may then manifest antigens not seen on remission and normal leukocytes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Specificity of Heteroantisera to Human Acute Leukemia-Associated Antigens

Baker¹,

Ramachandar²,

Taub³

1974

J. Clin. Invest.

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An evaluation of suspension culture systems for the growth of murine lymphoblastoid lines expressing TL and Thy‐1 alloantigens

Zwerner

Runyan

Cox

et al. 1975

Biotech & Bioengineering

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4s a prelude to our studies on T L and Thy-1 differentiation alloantigens, three rnurine Iymphoblastoid cell lines were examined for expression of these components. Optimal conditions for their m i i s culture were also determined. Several suspension culture systems were evaliiattd: a) 50 ml through 500 ml Wheaton and Bellco spinner flasks as well as 1, 4, and 8 liter Whcaton flasks modified for semicontinuous culture conditions, b) 3 lit.er Chemapec Vibrofermentor, and (1) 14 liter New Brunswick fermcntor. Utilizing these typcs of vessels the opt.iinal culture conditions were evaluat,ed as to Lhe effect. of: 1 ) pH, 2) initial concentration of cell inoculum, 3) t.ypes of media, and 4) methods of gmsing and gas mixtures on t,he rat.e of growth and alloant.igen expression. This study dcnionstrateti that cells could be cultured on a sernicont.inuous b:tsis up t.o densities of 2-4 X 106 cells/ml if a vessel of appropriate dimensions was utilized, t.he appropriate medium select.ed, and the p H controlled by CO, and air overlay. Once these paranieters were est,ablished the growth of a given cell line was highly reproducible. Under optimal culture conditions the expression of Thy-1 was maximum while t.he cells were in the exponential stage of growth and reduced during the lag and stationary p h u e s of growth. The expression of T L did not vary as significantly during the various stages of growth. One cell line grown in medium supplemented with 10c/r horse serum expressed less Thy-I than those grown in medium cont.aining 10%. fetal calf serum. The factors affecting cell growth and alloantigen expression have been considered in the design of a large-scale suspension cult.ure facility for cult.uring 1000 liters of cells per week.

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Variations in Expression of Cell Membrane Antigens by Cultured Cells

Cited by 10 publications

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Thymidine Dinucleotides Induce S Phase Cell Cycle Arrest in Addition to Increased Melanogenesis in Human Melanocytes

Thymidine Dinucleotides Induce S Phase Cell Cycle Arrest in Addition to Increased Melanogenesis in Human Melanocytes

Specificity of Heteroantisera to Human Acute Leukemia-Associated Antigens

An evaluation of suspension culture systems for the growth of murine lymphoblastoid lines expressing TL and Thy‐1 alloantigens

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