Variations in glutelin and high molecular weight endosperm proteins among subspecies of rice (Oryza sativa L.) detected by two-dimensional gel electrophoresis.

Abstract

Variations in endosperm polypeptides among 16 cultivars of rice were analyzed by two-dimensional gel electrophoresis. One glutelin alpha-subunit (alpha 8) with a molecular mass of 32.5 kDa was exclusively present in indica cultivars. By contrast, the glutelin alpha 3 subunit, with a molecular mass of 32 kDa, was present only in japonica and javanica cultivars. Glutelin alpha-subunits alpha 5a and alpha 5b, which have a molecular mass of 32 kDa and slightly different isoelectric points, differed among rice subspecies, i.e. the former was detected in japonica cultivars and the latter was detected in indica cultivars. Internal amino acid sequences of the indica-specific glutelin alpha-subunits alpha 5b and alpha 8, were analyzed with a gas-phase protein sequencer. Of 46 amino acid residues determined, only two residues differed, and they corresponded with the sequences deduced from type I and type II cDNAs of glutelin subfamily A, respectively. Nine polypeptides of higher molecular mass (35-90 kDa) also differed. In particular, two of these polypeptides designated B and C which were identical to Wx proteins with molecular masses of 58 kDa were strongly expressed in indica endosperms, but only weakly expressed in japonica and javanica. F1 seeds obtained from a cross between japonica and indica cultivars showed an intermediate intensity of Wx proteins and contained all nine glutelin alpha-subunits including both the japonica and indica types. In this experiment, Wx proteins designated B and C and glutelin alpha-subunits could be used to identify differences between japonica and indica cultivars.

Effect of storage proteins on pasting properties and microstructure of Thai rice

“…van der Borght et al (2006) reported the MW of a subunit as 32 kDa and b subunit as 25 kDa. The precursor band of around 58 kDa (Wx proteins) has been reported to express strongly in Indica cultivars compared to those of Japonica and Javanica (Abe, Gusti, Ono, & Sasahara, 1996). Accelerated aging did not have an apparent effect on the MW profiles of the extracted glutelin of Thai rice cultivars, both under non-reducing and reducing conditions.…”

Rice proteomics: A cornerstone for cereal food crop proteomes

“…In rice, protein quality is an important determining value in the nutrient value of cooked rice, and a first study to determine precise varietal and subspecific variations among japonica (6), indica (6), and javanica (4) cultivars, using rice-endosperm proteins was performed in 1996 (Abe et al, 1996). Variations in endosperm polypeptides among these rice cultivars were analyzed by 2-DGE.…”

Proteomics for genetic and physiological studies in plants

“…Several successful attempts were made, using 2-DE to characterize closely related lines of durum wheat [19], flour allergens in bread wheat cultivars [20], different lines of barley [21] differing in malting quality [22], cultivars of cultivated and wild rice [23,24], genotypes of maize [25,26], of sugar cane [27], of Vigna angularis [28] or of carrots [29]. Van Telgen and van Loon [30] examined the chromatin-associated proteins from leaves of different species and varieties of Nicotiana differing in their sensitivity towards tobacco mosaic virus (TMV).…”