Variations in seed germination of Hippophae salicifolia with different presoaking treatments

Abstract

Mature seeds of H. salicifolia, collected from five provenances (i.e. Hanuman Chatti, Helang, Lata, Rambara and Janggal Chatti) in west Himalaya, India, were treated with stratification (at 4ºC for 15, 30 and 60 days) and in different concentrations of GA 3 (5, 10, 20 mM), KNO 3 (50, 100, 200 mM) and Thiourea (50, 100, 200 mM) solution to determine the variations in seed germination. Results reveal that the germination rates of seeds from different provenances under different pre-sowing treatments are significantly increased compared to those in control (24%-30%). The seeds treated with Thiourea (100 mM) have highest germination rate (76%-83% for different seed sources), followed by those (63%-71% for different seed sources) pretreated with stratification (4 o C, 30 days). GA 3 treatment significantly shortens the mean germination time (MGT) and improves seed germination percentage. Considering the practical applicability and cost effectiveness, thiourea (100 mM) and stratification (at 4 o C) treatments for seed germination are recommended for mass multiplication through seeds of H. salicifolia in village/forest nurseries of the west Himalaya, India.

Effects of pre-sowing treatments on the germination and early growth performance of Pouteria campachiana

“…Different approaches of breaking seed dormancy in order to enhance germination rate and increase the germination process have been suggested by many authors (Airi et al 2009;Azad et al 2010). These include physical scarification of the seed coat by nicking, piercing with needles and knives, and/or using abrasion paper (Schmidt 2000).…”

Germination and short-term storage of Hippophae rhamnoides L. seeds and its ex-situ reintroduction potential assessment under North East Indian conditions

“…Under natural in vivo conditions, propagation becomes difficult as a result of various germination obstacles in Hippophae sp. seeds (Li and Schroeder 2003;Busing and Slabaugh 2008;Airi et al 2009;Frochot et al 2009). These obstacles are mainly because of hard seed coat or embryo dormancy that could be overcome by various chemical and mechanical treatments (Landis et al 1996).…”

“…Seeds also form the source and end material for genetic manipulation of plants. Efficiency of seed germination of H. salicifolia has been reported currently (Sankhyan et al 2005;Airi et al 2009). However, the literature cited in recent years does not deal with any comprehensive report on in vitro seed germination and storage of H. rhamnoides.…”

“…Seed germination in Hippophae sp. is also inhibited by several biotic factors such as hard coat and embryo dormancy (Landis et al 1996;Sankhyan et al 2005;Airi et al 2009;Frochot et al 2009). …”

Germinación y ruptura de latencia en semillas de Daphne giraldii Nitsche (Thymelaeaceae) provenientes del noroeste de China

“…Some researchers (Airi, Bhatt, Bhatt, Rawal, & Dhar, 2009;Azad, Rahman, & Matin, 2011) have studied different pre-sowing treatments for seed germination to break seed dormancy and thereby enhance the rate of germination and accelerate the germination process. Seed dormancy may be overcome by chemical treatments, incremental stratification and seed burial (Ooim, Auld, & Whelan 2006;Travlos et al, 2007;Merritt et al, 2007).…”