Variations in the Level of Urinary Thiobarbituric Acid Reactant in Healthy Humans under Different Physiological Conditions.

Abstract

The level of urinary thiobarbituric acid (TBA) reactants in healthy human subjects due to malonaldehyde derivatives was measured to assess the lipid peroxidation status of the whole body. For each subject the TBA reactant level over a day varied over a 2-3 fold range while the daily level varied over a 1.5-3 fold range under normal life-style conditions. One of the factors causing an increase in the reactant level within a single day may be the subjects's physical activity, because the reactant level of each subject was higher in the afternoon or in the evening than in the morning. Remaining awake all night or hard exercise caused a dramatic increase in the reactant level over a day and in the daily reactant level. The reactant level within a single day for a subject was increased 5.5 fold and the daily level 3 fold by remaining awake all night, and the level within a day was increased 22 fold by hard exercise while the corresponding daily level was increased 7 fold. It is unlikely that food, alcohol and smoking greatly affect the reactant level. The results suggest that increased physical activity enhances lipid peroxidation in the whole body and thus the increased urinary excretion of malonaldehyde derivatives.

Assessment of lifestyle effects on the overall antioxidant capacity of healthy subjects.

“…In agreement with this suggestion, in healthy subjects, remaining awake all night was responsible for an important increase in urine level of thiobarbituric acid-reactive substances, which are a known indicator of lipid peroxidation (37). Other experimental studies have demonstrated that psychologic stress decreases DNA repair (38) and inhibits radiation-induced apoptosis (39) in human blood leukocytes.…”

Violence Exposure, A Chronic Psychosocial Stressor, and Childhood Lung Function

“…Emotionally stressed rats have increased levels of 8-deoxy-hydroxy-guanosine (8-OhdG), a commonly used biomarker of oxidative stress (57). Similarly, staying awake all night increased the levels of thiobarbituric acid reactive substances (an indicator of lipid peroxidation) in humans (58). It is plausible that chronic stress may enhance airway inflammation through similar mechanisms to influence lung function.…”

Wheat Bran and Oat Bran Effectively Reduce Oxidative Stress Induced by High-Fat Diets in Pigs

“…In general, the blood MDA concentration is not regarded as a very precise parameter. Since lipid peroxidation products measured in excreted urine most probably indicate the total oxidative status of the whole body [22,23], the measurement of a 24-hour rate of MDA urine excretion is generally considered much more accurate than measurement of the MDA blood concentration. Additionally, the study by Yang et al [4] measured the serum MDA concentration.…”