Variations of the proteoglycans of the canine intervertebral disc with ageing

Cole, Tak-Chun; Ghosh, Peter; Taylor, T.K.F.

doi:10.1016/0304-4165(86)90082-6

Cited by 68 publications

(27 citation statements)

References 43 publications

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“…2Ϫ incorporation into newly synthesized proteoglycan (aggrecan) at different intervals after injection of radiolabeled materials (39)(40)(41)(42)(43). Such experiments cannot be done in humans and the results do not necessarily apply.…”

Section: Somentioning

confidence: 99%

The human lumbar intervertebral disc: evidence for changes in the biosynthesis and denaturation of the extracellular matrix with growth, maturation, ageing, and degeneration.

Antoniou

Steffen²,

Nelson³

et al. 1996

J. Clin. Invest.

949

842

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Very little is known about the turnover of extracellular matrix in the human intervertebral disc. We measured concentrations of specific molecules reflecting matrix synthesis and degradation in predetermined regions of 121 human lumbar intervertebral discs and correlated them with ageing and Thompson grade of degeneration.Synthesis in intervertebral discs, measured by immunoassay of the content of a putative aggrecan biosynthesis marker (846) and the content of types I and II procollagen markers, is highest in the neonatal and 2-5-yr age groups. The contents of these epitopes/molecules progressively diminished with increasing age. However, in the oldest age group (60-80 yr) and in highly degenerated discs, the type I procollagen epitope level increased significantly.The percentage of denatured type II collagen, assessed by the presence of an epitope that is exposed with cleavage of type II collagen, increased twofold from the neonatal discs to the young 2-5-yr age group. Thereafter, the percentage progressively decreased with increasing age; however, it increased significantly in the oldest group and in highly degenerate discs.We identified three matrix turnover phases. Phase I (growth) is characterized by active synthesis of matrix molecules and active denaturation of type II collagen. Phase II (maturation and ageing) is distinguished by a progressive drop in synthetic activity and a progressive reduction in denaturation of type II collagen. Phase III (degeneration and fibrotic) is illustrated by evidence for a lack of increased synthesis of aggrecan and type II procollagen, but also by an increase in collagen type II denaturation and type I procollagen synthesis, both dependent on age and grade of tissue degeneration. ( J. Clin. Invest. 1996. 98:996-1003.)

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Section: Somentioning

confidence: 99%

The human lumbar intervertebral disc: evidence for changes in the biosynthesis and denaturation of the extracellular matrix with growth, maturation, ageing, and degeneration.

Antoniou

Steffen²,

Nelson³

et al. 1996

J. Clin. Invest.

949

842

View full text Add to dashboard Cite

show abstract

“…A slow age-related dehydratation of disk tissues [1,26] could lead to an increase in hydrophobic contacts. Changes in concentration and composition of proteogly cans surrounding collagen [27][28][29][30] may also play a major role in the modification of its solubility.…”

Section: Discussionmentioning

confidence: 99%

Age-Related Changes in Collagen of Human Intervertebral Disks

Olczyk¹

1992

Gerontology

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Age-related changes of human intervertebral disk collagen have been investigated. A progressive, age-related accumulation of collagen, both in annulus fibrosus and in nucleus pulposus was found. A decrease in collagen solubility was observed during physiological aging. Up to the 3rd-4th decades of life, a significant increase in the aldehyde content in disk collagen has been observed. Nuclei pulposi of the prolapsed disk contain more collagen, and its solubility was higher in comparison to corresponding necropsy material. Some alterations in the collagen of prolapsed disks may be considered as symptomatic of accelerated aging.

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“…It is well-established that the intervertebral disc undergoes changes in composition and structure with age which result in a progression of degeneration [2,3,5,9,10,24,31,34,37,38] and an associated increase in the risk for injury and low back pain [1,5,14,30,37,39,42,43]. Over the last few decades, several studies have shown that changes in the mechanical environment of the disc can result in remodeling, breakdown, and reorganization of the extracellular matrix [ 1, 7,13,[18][19][20][21][22]29,32,33,35,41,44], leading in some cases to signs of accelerated disc degeneration.…”

Section: Introductionmentioning

confidence: 99%

Anabolic and catabolic mRNA levels of the intervertebral disc vary with the magnitude and frequency of in vivo dynamic compression

MacLean

Lee

Alini

et al. 2004

Journal Orthopaedic Research

170

181

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The goal of this study was to characterize the anabolic and catabolic mRNA response of the disc to dynamic loading to determine if variations in the magnitude and/or frequency of loading could elicit different cellular responses. Sixty-eight Wistar rats were instrumented with an Ilizarov-type device spanning caudal disc 8-9. Seventy-two hours after surgery, animals were anesthetized and loaded at either 1 or 0.2 MPa at a frequency of 1, 0.2 or 0.01 Hz for 2 h (6 groups). The surgical control (Sham) animals underwent anesthesia with no loading. Loaded (c8-9) and internal-control discs (c6-7 and c10-11) were dissected and annulus and nucleus tissue were separately analyzed by real-time RT-PCR for levels of anabolic (collagen-1 A1 , collagen-2A1, aggrecan) and catabolic (MMP-3, MMP-13, ADAMTs-4) mRNA. In the nucleus, a frequency-dependent response was seen at 1 MPa with anabolic genes stimulated at 0.01 Hz and catabolic genes at 1 Hz. In the annulus all frequencies resulted in significant up-regulation of catabolic mRNA at 1 MPa loading. In general loading at 0.2 MPa or 0.2 Hz had little effect on gene expression. The results suggest that gene expression of the annulus appears to be more dependent on the magnitude of applied stress, while the nucleus is both magnitude-and frequency-dependent.

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Variations of the proteoglycans of the canine intervertebral disc with ageing

Cited by 68 publications

References 43 publications

The human lumbar intervertebral disc: evidence for changes in the biosynthesis and denaturation of the extracellular matrix with growth, maturation, ageing, and degeneration.

The human lumbar intervertebral disc: evidence for changes in the biosynthesis and denaturation of the extracellular matrix with growth, maturation, ageing, and degeneration.

Age-Related Changes in Collagen of Human Intervertebral Disks

Anabolic and catabolic mRNA levels of the intervertebral disc vary with the magnitude and frequency of in vivo dynamic compression

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