Varied growth, biomass and cellulose content in tobacco expressing yeast-derived invertases

Canam, Thomas; Park, Jiyoung; Yu, Ka Yun; Campbell, Malcolm M.; Ellis, David; Mansfield, Shawn D.

doi:10.1007/s00425-006-0313-1

“…The authors observed a similar phenotype when the extracellular-located yeast invertase was driven under a meristem-specific promoter, which was observed in several other studies (e.g. Lerchl et al, 1995;Fukushima et al, 2001;Canam et al, 2006), confirming the principal function of a yeast-derived invertase in plants.…”

Section: Discussionsupporting

confidence: 79%

Regulation of Arbuscular Mycorrhization by Carbon. The Symbiotic Interaction Cannot Be Improved by Increased Carbon Availability Accomplished by Root-Specifically Enhanced Invertase Activity

Schaarschmidt

¹

,

González

²

,

Roitsch

³

et al. 2007

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The mutualistic interaction in arbuscular mycorrhiza (AM) is characterized by an exchange of mineral nutrients and carbon. The major benefit of AM, which is the supply of phosphate to the plant, and the stimulation of mycorrhization by low phosphate fertilization has been well studied. However, less is known about the regulatory function of carbon availability on AM formation. Here the effect of enhanced levels of hexoses in the root, the main form of carbohydrate used by the fungus, on AM formation was analyzed. Modulation of the root carbohydrate status was performed by expressing genes encoding a yeast (Saccharomyces cerevisiae)-derived invertase, which was directed to different subcellular locations. Using tobacco (Nicotiana tabacum) alcTcwINV plants, the yeast invertase was induced in the whole root system or in root parts. Despite increased hexose levels in these roots, we did not detect any effect on the colonization with Glomus intraradices analyzed by assessment of fungal structures and the level of fungus-specific palmitvaccenic acid, indicative for the fungal carbon supply, or the plant phosphate content. Roots of Medicago truncatula, transformed to express genes encoding an apoplast-, cytosol-, or vacuolar-located yeastderived invertase, had increased hexose-to-sucrose ratios compared to b-glucuronidase-transformed roots. However, transformations with the invertase genes did not affect mycorrhization. These data suggest the carbohydrate supply in AM cannot be improved by root-specifically increased hexose levels, implying that under normal conditions sufficient carbon is available in mycorrhizal roots. In contrast, tobacco rolCTppa plants with defective phloem loading and tobacco pyk10TInvInh plants with decreased acid invertase activity in roots exhibited a diminished mycorrhization.

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“…The authors observed a similar phenotype when the extracellular-located yeast invertase was driven under a meristem-specific promoter, which was observed in several other studies (e.g. Lerchl et al, 1995;Fukushima et al, 2001;Canam et al, 2006), confirming the principal function of a yeast-derived invertase in plants.…”

Section: Discussionsupporting

confidence: 79%

Regulation of Arbuscular Mycorrhization by Carbon. The Symbiotic Interaction Cannot Be Improved by Increased Carbon Availability Accomplished by Root-Specifically Enhanced Invertase Activity

Schaarschmidt

¹

,

González²,

Roitsch³

et al. 2007

View full text Add to dashboard Cite

The mutualistic interaction in arbuscular mycorrhiza (AM) is characterized by an exchange of mineral nutrients and carbon. The major benefit of AM, which is the supply of phosphate to the plant, and the stimulation of mycorrhization by low phosphate fertilization has been well studied. However, less is known about the regulatory function of carbon availability on AM formation. Here the effect of enhanced levels of hexoses in the root, the main form of carbohydrate used by the fungus, on AM formation was analyzed. Modulation of the root carbohydrate status was performed by expressing genes encoding a yeast (Saccharomyces cerevisiae)-derived invertase, which was directed to different subcellular locations. Using tobacco (Nicotiana tabacum) alcTcwINV plants, the yeast invertase was induced in the whole root system or in root parts. Despite increased hexose levels in these roots, we did not detect any effect on the colonization with Glomus intraradices analyzed by assessment of fungal structures and the level of fungus-specific palmitvaccenic acid, indicative for the fungal carbon supply, or the plant phosphate content. Roots of Medicago truncatula, transformed to express genes encoding an apoplast-, cytosol-, or vacuolar-located yeastderived invertase, had increased hexose-to-sucrose ratios compared to b-glucuronidase-transformed roots. However, transformations with the invertase genes did not affect mycorrhization. These data suggest the carbohydrate supply in AM cannot be improved by root-specifically increased hexose levels, implying that under normal conditions sufficient carbon is available in mycorrhizal roots. In contrast, tobacco rolCTppa plants with defective phloem loading and tobacco pyk10TInvInh plants with decreased acid invertase activity in roots exhibited a diminished mycorrhization.Arbuscular mycorrhiza (AM) represents a widespread mutualistic association between soil-born fungi of the phylum Glomeromycota and most land plants. The AM interaction enables the plant to improve its supply of water and mineral nutrients, mainly phosphate. In return, the obligate biotrophic AM fungi are provided with carbon. In the Arum-type interaction, as analyzed here between Glomus sp. and tobacco (Nicotiana tabacum) or Medicago truncatula, the AM fungus colonizes the cortical cells by formation of intra-and intercellular hyphae and very characteristic haustorialike structures, the highly branched intracellular arbuscules. When the carbon supply is sufficient, lipid-rich vesicles are formed intercellularly within the cortex, as fungal storage organs. Intracellular fungal structures are separated from the plant cytoplasm by an extension of the plasma membrane, forming the periarbuscular membrane surrounding the arbuscule. The greatly increased surfaces of host and arbuscule plasma membranes offers optimized conditions for effective nutrient exchange via the established symbiotic interface (for review, see Gianinazzi-Pearson et al., 1996;Harrison, 1999).The exchange of phosphate via the periarbuscular interface and th...

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“…Overexpression of these two genes together significantly increases plant height, with postulated increase in cellulose content and overall plant biomass. Similarly, heterologous expression of invertase in tobacco plants resulted in significant increase in biomass and in cellulose content [90]. Moreover, SPS overexpression led to enhanced plant growth and fiber elongation in tobacco models which can be explained by elevated concentrations of sink sucrose pools compared to wild-type plants [91].…”

Section: Genetic Manipulation Of the Cellulose Synthesis Pathwaymentioning

confidence: 99%

Plant cell wall reconstruction toward improved lignocellulosic production and processability

Abramson

¹

,

Shoseyov

²

,

Shani³

2010

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Varied growth, biomass and cellulose content in tobacco expressing yeast-derived invertases

Cited by 29 publications

References 37 publications

Regulation of Arbuscular Mycorrhization by Carbon. The Symbiotic Interaction Cannot Be Improved by Increased Carbon Availability Accomplished by Root-Specifically Enhanced Invertase Activity

Regulation of Arbuscular Mycorrhization by Carbon. The Symbiotic Interaction Cannot Be Improved by Increased Carbon Availability Accomplished by Root-Specifically Enhanced Invertase Activity

Regulation of Arbuscular Mycorrhization by Carbon. The Symbiotic Interaction Cannot Be Improved by Increased Carbon Availability Accomplished by Root-Specifically Enhanced Invertase Activity

Plant cell wall reconstruction toward improved lignocellulosic production and processability

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