Various antibacterial mechanisms of biosynthesized copper oxide nanoparticles against soilborne<i>Ralstonia solanacearum</i>

Chen, Juanni; Mao, Shuyu; Xu, Zhijian; Ding, Wei

doi:10.1039/c8ra09186b

Cited by 131 publications

(45 citation statements)

References 70 publications

(88 reference statements)

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“…CuO NPs at a 50 µg/mL concentration significantly inhibited the growth of total oral bacteria, extracellular polysaccharide (EPS) production, and biofilm formation on the glass, acrylic dentures, and cultured human epithelial cells as models [ 100 ]. It has also been shown that CuO NPs’ inhibitory concentration on Ralstonia solanacearum biofilms is 125 and 250 µg/mL after an incubation period of 24 and 72 h [ 101 ]. Thus, it is evident that CuO NPs can be potentially effective against biofilms, as shown in different groups of microorganisms.…”

Section: Effects Of Metal Oxide Nps On Plankton Cells and Biofilmmentioning

confidence: 99%

Metal Oxide Nanoparticles Against Bacterial Biofilms: Perspectives and Limitations

2020

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At present, there is an urgent need in medicine and industry to develop new approaches to eliminate bacterial biofilms. Considering the low efficiency of classical approaches to biofilm eradication and the growing problem of antibiotic resistance, the introduction of nanomaterials may be a promising solution. Outstanding antimicrobial properties have been demonstrated by nanoparticles (NPs) of metal oxides and their nanocomposites. The review presents a comparative analysis of antibiofilm properties of various metal oxide NPs (primarily, CuO, Fe3O4, TiO2, ZnO, MgO, and Al2O3 NPs) and nanocomposites, as well as mechanisms of their effect on plankton bacteria cells and biofilms. The potential mutagenicity of metal oxide NPs and safety problems of their wide application are also discussed.

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Section: Effects Of Metal Oxide Nps On Plankton Cells and Biofilmmentioning

confidence: 99%

Metal Oxide Nanoparticles Against Bacterial Biofilms: Perspectives and Limitations

2020

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“…On the one hand, by comparison to silver (Xiu et al, 2012), one might assume that it is only soluble copper, not particles, that are antimicrobial. On the other hand, increased activities of copper-based nanoparticles, above and beyond just copper ion content, have been indicated (Chen et al, 2019;Raffi et al, 2010;Sarkar et al, 2012). Indeed, the issue of whether nanoparticles per se have specific activity against bacteria, remains unclear but important.…”

Section: Introductionmentioning

confidence: 99%

Copper nanoparticles have negligible direct antibacterial impact

et al. 2020

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Introduction: Soluble copper that can be acquired by bacteria is toxic and therefore antimicrobial. Whether nanostructured copper materials, in either disperse or agglomerated form, have antimicrobial impact, aside from that of their dissolution products, is not clear and was herein addressed. Methods: We took five nanostructured copper materials, two metallic, and three oxo-hydroxides with one of these being silicate-substituted. Four agglomerated in the bacterial growth media whilst the silicate-substituted material remained disperse and small (6.5 nm diameter). Antibacterial activity against E. coli was assessed with copper phase distribution measured over time. Using the dose of soluble copper, and benchmark dose non-linear regression modelling, we determined how well this phase predicted antimicrobial activity. Finally, we used Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) analysis to investigate whether membrane adhesion effects by copper were plausible or if intracellular uptake most likely explained the bacterial impact of copper. Results: Comparison over time of antimicrobial activity against particulate or soluble phases of the aquated materials clearly demonstrated that soluble copper but not particulate forms were associated with inhibition of bacterial growth. Indeed, the benchmark dose modelling showed the soluble dose required to cause a 50% reduction in E. coli growth was strongly clustered -for all particle formulations -at 14.5 mg/L (10-19 mg/L 90% confidence interval). By comparison, total copper levels associated with the same reduction in viability varied widely (45-549 mg/L). Finally, in favour of this soluble product dominance in terms of antimicrobial activity, copper had low association with bacterial membrane (something both soluble and particulate materials could do) but showed high intra-bacterial levels (something only soluble copper could do). Conclusion: Taken together our data show that it is the uptake of soluble but not particulate copper, and the intracellular loading not just contact and membrane association, that drives copper toxicity to bacteria. Therapeutic strategies for novel antimicrobial copper compounds should consider these findings.

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“…First, copper acts on a similar principle to silver, as it combines with the thiol groups of key microbial enzymes and inactivates their functions [ 97 ]. Second, the Cu + ion from cuprous oxide (Cu 2 O) forms complexes with the peptides found within the microbial membranes [ 98 ]. Third, the dissociation of Cu 2+ from cupric oxide (CuO) induces ROS generation, leading to disturbances in amino acid and nucleic acid biosynthesis and the associated biochemical processes, e.g., iron displacement from iron–sulfur clusters and zinc or other metal ions competition for protein binding sites, disruption of the microbial membrane, and blocking of cellular respiration [ 60 , 95 , 96 , 98 , 99 ].…”

Section: Inorganic Nanoparticles With Antimicrobial Propertiesmentioning

confidence: 99%

Inorganic Nanoparticles and Composite Films for Antimicrobial Therapies

Spirescu

Chircov

Grumezescu

et al. 2021

IJMS

106

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The development of drug-resistant microorganisms has become a critical issue for modern medicine and drug discovery and development with severe socio-economic and ecological implications. Since standard and conventional treatment options are generally inefficient, leading to infection persistence and spreading, novel strategies are fundamentally necessary in order to avoid serious global health problems. In this regard, both metal and metal oxide nanoparticles (NPs) demonstrated increased effectiveness as nanobiocides due to intrinsic antimicrobial properties and as nanocarriers for antimicrobial drugs. Among them, gold, silver, copper, zinc oxide, titanium oxide, magnesium oxide, and iron oxide NPs are the most preferred, owing to their proven antimicrobial mechanisms and bio/cytocompatibility. Furthermore, inorganic NPs can be incorporated or attached to organic/inorganic films, thus broadening their application within implant or catheter coatings and wound dressings. In this context, this paper aims to provide an up-to-date overview of the most recent studies investigating inorganic NPs and their integration into composite films designed for antimicrobial therapies.

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Various antibacterial mechanisms of biosynthesized copper oxide nanoparticles against soilborneRalstonia solanacearum

Abstract: Green synthesized CuONPs disturb cell metabolism, biofilm formation, physical motility and gene expression in Ralstonia solanacearum, thereby effectively controlling bacterial wilt.

Cited by 131 publications

References 70 publications

Metal Oxide Nanoparticles Against Bacterial Biofilms: Perspectives and Limitations

Metal Oxide Nanoparticles Against Bacterial Biofilms: Perspectives and Limitations

Copper nanoparticles have negligible direct antibacterial impact

Inorganic Nanoparticles and Composite Films for Antimicrobial Therapies

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