1997
DOI: 10.1084/jem.186.4.589
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Vascular Adhesion Protein 1 (VAP-1) Mediates Lymphocyte Subtype-specific, Selectin-independent Recognition of Vascular Endothelium in Human Lymph Nodes

Abstract: Interactions between lymphocyte surface receptors and their ligands on vascular endothelial cells regulate the exit of lymphocytes from the circulation. Distinct subsets of mononuclear cells bind to high endothelial venules (HEVs) in different lymphoid organs to a different extent, but the molecular mechanisms behind this selectivity have remained poorly characterized. Here we show that vascular adhesion protein-1 (VAP-1) mediates subtype-specific binding of CD8-positive T cells and natural killer cells to hum… Show more

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Cited by 105 publications
(83 citation statements)
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“…Note that the data for Table II was counted using darkfield microscopy of unstained cells, and hence the results (binding avidity of different cell types) can be readily compared with those obtained from frozen section assays in which unstained cells were used as well. vessels in lymph nodes, nor does it support synovial adherence of macrophages isolated from noninflamed gut (6,15). Hence, in the inflamed gut some nonphysiological stimuli involved in the synthesis of the as yet uncharacterized leukocyte ligand of VAP-1 apparently takes place, possibly on those macrophage subtypes that appear to be unique for IBD intestine (37).…”
Section: Discussionmentioning
confidence: 98%
See 1 more Smart Citation
“…Note that the data for Table II was counted using darkfield microscopy of unstained cells, and hence the results (binding avidity of different cell types) can be readily compared with those obtained from frozen section assays in which unstained cells were used as well. vessels in lymph nodes, nor does it support synovial adherence of macrophages isolated from noninflamed gut (6,15). Hence, in the inflamed gut some nonphysiological stimuli involved in the synthesis of the as yet uncharacterized leukocyte ligand of VAP-1 apparently takes place, possibly on those macrophage subtypes that appear to be unique for IBD intestine (37).…”
Section: Discussionmentioning
confidence: 98%
“…Normally L-, E-, and P-selectins and their carbohydrate-containing mucin-like counterparts (like P-selectin glycoprotein ligand-1 (PSGL-1) for P-and E-selectin, and peripheral lymph node addressins (PNAd) for L-selectin) mediate the initial phases of extravasation (13). However, CD44 and vascular adhesion protein-1 (VAP-1) also contribute to the rolling of blood-borne cells (14,15). Chemokines are thought to be the key players in converting loosely rolling cells into stably bound cells (16).…”
mentioning
confidence: 99%
“…An increase in the percentage of bound lymphocytes of all input lymphocytes subsequent to CD73 engagement in our binding experiments reflects the size of the CD73-positive cell population. To date, most studies concerning molecules involved in lymphocyte binding to endothelial cells have measured the binding of either unseparated lymphocytes or well-defined lymphocyte populations such as CD4-, CD8-, or CD19-positive cells, and of the studied molecules only the contribution of vascular adhesion protein-1 seems to be subtype restricted, i.e., it preferentially mediates adherence of CD8-positive cells to the endothelium (34). Our present results suggest that trafficking of lymphocytes in the body is not regulated only by the subtype-specific manner that is based on the conventional phenotype definition of the lymphocyte subpopulations.…”
Section: Discussionmentioning
confidence: 99%
“…It is an endothelial adhesion molecule that is up-regulated at sites of inflammation and mediates lymphocyte binding to inflamed endothelium (6). VAP-1 is preferentially involved in adherence of CD8 ϩ (T killer) and CD16 ϩ (NK) lymphocytes to high endothelial venules (HEV) in peripheral lymph nodes and tonsils (7). Since TIL activated according to our protocol are predominantly CD8 ϩ (2,8), it would be possible that VAP-1 participates in homing of TIL.…”
mentioning
confidence: 99%