Vascular and plaque imaging with ultrasmall superparamagnetic particles of iron oxide

Alam, Shirjel; Stirrat, Colin; Richards, Jennifer; Mirsadraee, Saeed; Semple, Scott; Tse, George; Henriksen, Peter; Newby, David E.

doi:10.1186/s12968-015-0183-4

Cited by 44 publications

(39 citation statements)

References 69 publications

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“…TEM images showed that labeled ASCs incorporate USPIO by endocytic mechanisms, as already reported. 29 It has been demonstrated that nanoparticles internalized by endocytosis often accumulate inside multivesicular bodies, 30 which, in turn, may fuse with the plasma membrane thus releasing their cargo (exosomes). 31 It is likely that USPIO undergo the same secretion process of the endosomal system.…”

Section: Discussionmentioning

confidence: 99%

Magnetic resonance imaging of ultrasmall superparamagnetic iron oxide-labeled exosomes from stem cells: a new method to obtain labeled exosomes

Busato

Bonafede

Bontempi

et al. 2016

IJN

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Purpose Recent findings indicate that the beneficial effects of adipose stem cells (ASCs), reported in several neurodegenerative experimental models, could be due to their paracrine activity mediated by the release of exosomes. The aim of this study was the development and validation of an innovative exosome-labeling protocol that allows to visualize them with magnetic resonance imaging (MRI). Materials and methods At first, ASCs were labeled using ultrasmall superparamagnetic iron oxide nanoparticles (USPIO, 4–6 nm), and optimal parameters to label ASCs in terms of cell viability, labeling efficiency, iron content, and magnetic resonance (MR) image contrast were investigated. Exosomes were then isolated from labeled ASCs using a standard isolation protocol. The efficiency of exosome labeling was assessed by acquiring MR images in vitro and in vivo as well as by determining their iron content. Transmission electron microscopy images and histological analysis were performed to validate the results obtained. Results By using optimized experimental parameters for ASC labeling (200 µg Fe/mL of USPIO and 72 hours of incubation), it was possible to label 100% of the cells, while their viability remained comparable to unlabeled cells; the detection limit of MR images was of 10 2 and 2.5×10 3 ASCs in vitro and in vivo, respectively. Exosomes isolated from previously labeled ASCs retain nanoparticles, as demonstrated by transmission electron microscopy images. The detection limit by MRI was 3 µg and 5 µg of exosomes in vitro and in vivo, respectively. Conclusion We report a new approach for labeling of exosomes by USPIO that allows detection by MRI while preserving their morphology and physiological characteristics.

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Section: Discussionmentioning

confidence: 99%

Magnetic resonance imaging of ultrasmall superparamagnetic iron oxide-labeled exosomes from stem cells: a new method to obtain labeled exosomes

Busato

Bonafede

Bontempi

et al. 2016

IJN

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Section: Iron Oxide Nanoparticles Imaging Of Plaque Inflammationmentioning

confidence: 99%

“…Based on their size, they are subdivided into several types, better suited for different applications (for example intravascular versus tissue imaging)(2). Their tissue accumulation causes local magnetic field inhomogeneities that can be measured by MRI (2). Alternatively, iron oxides (such as the dextran coated cross-linked iron oxide nanoparticles, CLIO, used in this work)(3), can be labeled with radioisotopes and/or fluorophores and functionalized as multi-modality imaging agents detectable by positron emission tomography (PET) or near infra-red fluorescence (NIRF) imaging, alone or in combination(4).…”

Section: Iron Oxide Nanoparticles Imaging Of Plaque Inflammationmentioning

confidence: 99%

“…Alternatively, iron oxides (such as the dextran coated cross-linked iron oxide nanoparticles, CLIO, used in this work)(3), can be labeled with radioisotopes and/or fluorophores and functionalized as multi-modality imaging agents detectable by positron emission tomography (PET) or near infra-red fluorescence (NIRF) imaging, alone or in combination(4). Iron oxides can be used as non-targeted agents, avidly phagocytized by inflammatory cells and cells of the reticulo endothelial system (2), or can be labeled to target and image specific molecules involved in disease processes (some examples in atherosclerosis are iron oxides targeted to oxidation specific epitopes, or to adhesion molecules on endothelial cells)(5). While targeted and functionalized nanoparticles are typically used in animal models, some non-targeted iron oxides are commercially available.…”

Section: Iron Oxide Nanoparticles Imaging Of Plaque Inflammationmentioning

confidence: 99%

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Intraplaque and Cellular Distribution of Dextran-Coated Iron Oxide Fluorescently Labeled Nanoparticles

Calcagno

Fayad

2017

Circ: Cardiovascular Imaging

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“…T2 and T2*-weighted imaging provide a sensitive method of assessing USPIO accumulation in tissues. In this concise and well-written review, the authors address the basic concepts, developments and applications of novel USPIOS contrast agents for CMR studies of vascular inflammation and atherosclerotic plaques imaging [142]. …”

Section: Vascular Diseasementioning

confidence: 99%

Review of Journal of Cardiovascular Magnetic Resonance 2015

Pennell

Baksi

Prasad

et al. 2016

Journal of Cardiovascular Magnetic Resonance

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There were 116 articles published in the Journal of Cardiovascular Magnetic Resonance (JCMR) in 2015, which is a 14 % increase on the 102 articles published in 2014. The quality of the submissions continues to increase. The 2015 JCMR Impact Factor (which is published in June 2016) rose to 5.75 from 4.72 for 2014 (as published in June 2015), which is the highest impact factor ever recorded for JCMR. The 2015 impact factor means that the JCMR papers that were published in 2013 and 2014 were cited on average 5.75 times in 2015. The impact factor undergoes natural variation according to citation rates of papers in the 2 years following publication, and is significantly influenced by highly cited papers such as official reports. However, the progress of the journal's impact over the last 5 years has been impressive. Our acceptance rate is <25 % and has been falling because the number of articles being submitted has been increasing. In accordance with Open-Access publishing, the JCMR articles go on-line as they are accepted with no collating of the articles into sections or special thematic issues. For this reason, the Editors have felt that it is useful once per calendar year to summarize the papers for the readership into broad areas of interest or theme, so that areas of interest can be reviewed in a single article in relation to each other and other recent JCMR articles. The papers are presented in broad themes and set in context with related literature and previously published JCMR papers to guide continuity of thought in the journal. We hope that you find the open-access system increases wider reading and citation of your papers, and that you will continue to send your quality papers to JCMR for publication.

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Vascular and plaque imaging with ultrasmall superparamagnetic particles of iron oxide

Cited by 44 publications

References 69 publications

Magnetic resonance imaging of ultrasmall superparamagnetic iron oxide-labeled exosomes from stem cells: a new method to obtain labeled exosomes

Magnetic resonance imaging of ultrasmall superparamagnetic iron oxide-labeled exosomes from stem cells: a new method to obtain labeled exosomes

Intraplaque and Cellular Distribution of Dextran-Coated Iron Oxide Fluorescently Labeled Nanoparticles

Review of Journal of Cardiovascular Magnetic Resonance 2015

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