Vascular Biology of Smooth Muscle Cells and Restenosis

Nankivell, Victoria; Primer, K.; Vidanapathirana, Achini K.; Psaltis, Peter J.; Bursill, Christina A.

doi:10.1007/978-3-030-43683-4_6

Cited by 2 publications

(2 citation statements)

References 68 publications

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“…Women with a history of IRSA are also at greater risk for having a premature coronary event such as coronary arterial disease and restenosis after the affected pregnancy ( Veerbeek et al., 2016 ; Brosens et al., 2017 ; Benagiano et al., 2021 ). A common link between these cardiovascular disorders and IRSA is the dysregulation of vascular smooth muscle cell (VSMC) plasticity ( Whitley and Cartwright, 2010 ; Brosens et al., 2011 ; Low et al., 2019 ; Nankivell et al., 2020 ). In the systemic vasculature, inappropriate VSMC responses to transforming growth factor beta (TGF-β) signaling are implicated in both coronary arterial disease and restenosis ( Suwanabol et al., 2011 ; Low et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Differential affinity chromatography reveals a link between Porphyromonas gingivalis–induced changes in vascular smooth muscle cell differentiation and the type 9 secretion system

Phillips

Reyes

2022

Front. Cell. Infect. Microbiol.

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Porphyromonas gingivalis is implicated in adverse pregnancy outcome. We previously demonstrated that intrauterine infection with various strains of P. gingivalis impairs the physiologic remodeling of the uterine spiral arteries (IRSA) during pregnancy, which underlies the major obstetrical syndromes. Women diagnosed with IRSA also have a greater risk for premature cardiovascular disease in later life. The dysregulated plasticity of vascular smooth muscle cells (VSMCs) is present in both IRSA and premature cardiovascular events. We hypothesized that VSMCs could serve as a bait to identify P. gingivalis proteins associated with dysregulated VSMC plasticity as seen in IRSA. We first confirmed that dams with P. gingivalis A7UF-induced IRSA also show perturbed aortic smooth muscle cell (AoSMC) plasticity along with the P. gingivalis colonization of the tissue. The in vitro infection of AoSMCs with IRSA-inducing strain A7UF also perturbed AoSMC plasticity that did not occur with infection by non-IRSA-inducing strain W83. Far-Western blotting with strain W83 and strain A7UF showed a differential binding pattern to the rat aorta and primary rat AoSMCs. The affinity chromatography/pull-down assay combined with mass spectrometry was used to identify P. gingivalis/AoSMC protein interactions specific to IRSA. Membrane proteins with a high binding affinity to AoSMCs were identified in the A7UF pull-down but not in the W83 pull-down, most of which were the outer membrane components of the Type 9 secretion system (T9SS) and T9SS cargo proteins. Additional T9SS cargo proteins were detected in greater abundance in the A7UF pull-down eluate compared to W83. None of the proteins enriched in the W83 eluate were T9SS components nor T9SS cargo proteins despite their presence in the prey preparations used in the pull-down assay. In summary, differential affinity chromatography established that the components of IRSA-inducing P. gingivalis T9SS as well as its cargo directly interact with AoSMCs, which may play a role in the infection-induced dysregulation of VSMC plasticity. The possibility that the T9SS is involved in the microbial manipulation of host cell events important for cell differentiation and tissue remodeling would constitute a new virulence function for this system.

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Section: Introductionmentioning

confidence: 99%

Differential affinity chromatography reveals a link between Porphyromonas gingivalis–induced changes in vascular smooth muscle cell differentiation and the type 9 secretion system

Phillips

Reyes

2022

Front. Cell. Infect. Microbiol.

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“…The main clinical risk after stent insertion, therefore, remains high platelet adhesion and activation, directly connected to stent-thrombosis; at the site of injury, which is common in stenting procedures, platelets quickly adhere and start to form a plug and blood coagulation that leads to vessel occlusion [ 14 ]. Besides, the uncontrolled proliferation of smooth muscle cells with high risk leads to the narrowing of a blood vessel and restricts blood flow [ 15 ]. These conditions can further lead to heart attack and stroke [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Bio-Performance of Hydrothermally and Plasma-Treated Titanium: The New Generation of Vascular Stents

Benčina

Rawat

Lakota

et al. 2021

IJMS

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The research presented herein follows an urgent global need for the development of novel surface engineering techniques that would allow the fabrication of next-generation cardiovascular stents, which would drastically reduce cardiovascular diseases (CVD). The combination of hydrothermal treatment (HT) and treatment with highly reactive oxygen plasma (P) allowed for the formation of an oxygen-rich nanostructured surface. The morphology, surface roughness, chemical composition and wettability of the newly prepared oxide layer on the Ti substrate were characterized by scanning electron microscopy (SEM) with energy-dispersive X-ray analysis (EDX), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and water contact angle (WCA) analysis. The alteration of surface characteristics influenced the material’s bio-performance; platelet aggregation and activation was reduced on surfaces treated by hydrothermal treatment, as well as after plasma treatment. Moreover, it was shown that surfaces treated by both treatment procedures (HT and P) promoted the adhesion and proliferation of vascular endothelial cells, while at the same time inhibiting the adhesion and proliferation of vascular smooth muscle cells. The combination of both techniques presents a novel approach for the fabrication of vascular implants, with superior characteristics.

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Vascular Biology of Smooth Muscle Cells and Restenosis

Cited by 2 publications

References 68 publications

Differential affinity chromatography reveals a link between Porphyromonas gingivalis–induced changes in vascular smooth muscle cell differentiation and the type 9 secretion system

Differential affinity chromatography reveals a link between Porphyromonas gingivalis–induced changes in vascular smooth muscle cell differentiation and the type 9 secretion system

Bio-Performance of Hydrothermally and Plasma-Treated Titanium: The New Generation of Vascular Stents

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