Vascular composition, apoptosis, and expression of angiogenic factors in the corpus luteum during prostaglandin F2α-induced regression in sheep

Vonnahme, K. A.; Redmer, Dale A.; Borowczyk, Ewa; Bilski, Jerzy J.; Luther, J. S.; Johnson, Mary Lynn; Reynolds, Lawrence P.; Grazul-Bilska, Anna T.

doi:10.1530/rep.1.01062

Cited by 69 publications

(69 citation statements)

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“…6). Apoptotic cells were also detected in ovine luteal tissues serving as a control (data not shown; see Vonnahme et al 2006, Grazul-Bilska et al 2008. Appearance of apoptotic cells in fetal ovaries was not affected by nutritional treatment.…”

Section: Resultsmentioning

confidence: 82%

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Cellular proliferation and vascularization in ovine fetal ovaries: effects of undernutrition and selenium in maternal diet

Grazul-Bilska¹,

Caton²,

Arndt³

et al. 2009

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Sheep were fed a maintenance (M) diet with adequate (A) Se or high (H) Se concentration from 21 days before breeding to day 135 of pregnancy. From day 50 to day 135 of pregnancy (tissue collection day), a portion of the ewes from ASe and HSe groups were fed restricted (R; 60% of M) diet. Fetal ovarian sections were stained for: 1) the presence of proliferating cell nuclear antigen (a marker of proliferating cells) to determine the proportion of proliferating primordial follicles, or the labeling index (LI; percentage of proliferating cells) for primordial, primary, secondary and antral follicles, stromal tissues, and blood vessels; 2) factor VIII (a marker of endothelial cells) or 3) a presence of apoptotic cells/bodies. The number of proliferating primordial follicles and the LI of primordial follicles was decreased by R and/or HSe diets. The LI was similar for theca and granulosa cells, and for secondary or antral follicles, but was greater in secondary and antral than in primordial and primary follicles. R diet and/or Se affected the LI in all follicle types, in stromal tissues and blood vessels. A dense network of blood vessels was detected in the areas containing secondary to antral follicles, medulla, and hilus, but areas containing primordial follicles were poorly vascularized. The number of apoptotic cells was minimal. These results demonstrate that nutrient restriction and/or Se level in the maternal diet affected cellular proliferation in follicles, blood vessels, and stromal tissues in fetal ovaries. Thus, plane of nutrition and Se in the maternal diet may impact fetal ovarian development and function.

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Section: Resultsmentioning

confidence: 82%

“…As a positive control, tissues sections from the corpora lutea undergoing luteolysis were used as described previously (Vonnahme et al 2006).…”

Section: Tissue Collection and Immunohistochemistrymentioning

confidence: 99%

Cellular proliferation and vascularization in ovine fetal ovaries: effects of undernutrition and selenium in maternal diet

Grazul-Bilska¹,

Caton²,

Arndt³

et al. 2009

Reproduction

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“…However, while the mRNA expression for VEGFR1 is maintained, expression of VEGFR2 declines on day 18 of the cycle. Similarly, Vonnahme et al (2006) show VEGFR1 mRNA expression unchanged 12 h after PGF2α injection, whereas VEGFR2 was reduced 4 h after treatment.…”

Section: Discussionmentioning

confidence: 78%

“…Nonetheless, our results are in physiological agreement with the events that occur at luteolysis. In the ovine CL, PGF2α produced an increase in total VEGF mRNA expression 4 and 8 h after its injection, however, 24 h after PGF2α injection the expression of VEGF was below that of CLs collected before treatment (Vonnahme et al, 2006). PGF2α is released by the endometrium from days 15 to 19 of the bovine oestrous cycle (Ginther et al, 2010).…”

Section: Discussionmentioning

confidence: 95%

The corpora lutea proangiogenic state of VEGF system components is turned to antiangiogenic at the later phase of the oestrous cycle in cows

Guzmán

Macías-Valencia

Fierro

et al. 2015

Animal

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Blood vessel expansion and reduction in the corpus luteum (CL) is regulated by the vascular endothelial growth factor (VEGF) system and linked to the maintenance of the CL. The VEGF system has both angiogenic and antiangiogenic ligands and receptors. Our objective was to evaluate the relationship between the mRNA expression of angiogenic and antiangiogenic members of the VEGF system in the CL, throughout the luteal phase of the oestrous cycle in cows. The CL of 18 cows were collected by transvaginal surgery on days 4, 6, 9, 12, 15 and 18 of the oestrous cycle and the mRNA expression of VEGF system components was evaluated by quantitative real-time PCR. The mRNA expression of VEGF ligands and receptors increased (P < 0.05) from the early-and mid-luteal phase (days 4 to 12) reaching its maximum expression on day 15 of the cycle. We found no expression of VEGF164b throughout the cycle. Expression of sVEGFR1 did not change during the oestrous cycle and exceeded that of the VEGFR1 by 100 times. Nonetheless, as VEGFR1 increased, the relationship between the soluble and membrane receptor decreased (P < 0.01). In contrast, the expression of VEGFR2 was higher than that of its soluble isoform for all days studied, however, the ratio between the membrane-bound and its soluble counterpart decreased continuously throughout the cycle (P < 0.01). Our results show that the expression levels for VEGF ligands, receptors and their antagonistic counterparts are adjusted during CL development and regression, to upregulate angiogenesis early in the oestrous cycle and restrict it at the time of luteolysis.

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“…2). In other species, injection of PGF2α consistently stimulated eNOS mRNA expression and/or NOS activity within the CL of sheep [42], rat [23], and rabbit [22]. Moreover, PGF2α upregulates NOS activity in mature CL (Day 9) but not in immature CL (Day 4) of pseudopregnant rabbits [35].…”

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confidence: 89%

Nitric Oxide and Luteal Blood Flow in the Luteolytic Cascade in the Cow

Shirasuna

2010

Journal of Reproduction and Development

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Abstract. The corpus luteum (CL) of the estrous cycle in the cow is a dynamic organ which has a lifespan of approximately 17-18 days. The main function of the CL is to produce progesterone (P) that requires for achievement and maintenance of pregnancy. If pregnancy does not occur successfully, the CL must regress within a few days to induce the next chance of ovulation. As the CL matures, the steroidogenic cells establish contact with many capillary vessels, consequently the CL is composed of a large number of vascular endothelial cells that can account for up to 50% of all cells. Therefore, blood vessels and blood flow within the CL have an essential role in luteal function. Nitric oxide (NO), a strong vasorelaxant, is now known to play key roles in a variety of physiological process. Indeed, NO has established itself as a polyvalent molecule which plays a decisive role in regulating multiple functions within female reproductive system. In the CL, NO is produced and regulates luteal blood flow, P secretion and apoptosis of luteal cells as well as endothelial cells. This review describes the current investigation for possible roles of NO in the luteolytic cascade within the bovine CL. Key words: Corpus luteum, Luteal blood flow, Luteolysis, Nitric oxide (J. Reprod. Dev. 56: [9][10][11][12][13][14] 2010) he corpus luteum (CL) is a transient organ in the ovary of mammals. The bovine CL rapidly develops within 2-3 days after ovulation, which is accompanied by an active angiogenesis and vascularization from the preovulatory follicle. The main function of the CL is to produce progesterone (P) that requires for achievement and maintenance of pregnancy [1]. If pregnancy does not occur successfully, the CL is only functional for 17-18 days and it must regress within a few days to induce the next chance of ovulation. In ruminants, it is well known that a pulsatile release of prostaglandin F2α (PGF2α) from the uterus on days 17-18 of the estrous cycle is essential to induce regression of the CL [2]. Luteolytic PGF2α induces a drastic decrease in P release from the CL as well as CL volume and blood flow to the CL in the non-pregnant cow [3,4]. Thus, it is easily considered that the bovine CL has well designed mechanisms by which development and regression are effectively controlled.In the cow, the CL is composed of a large number of vascular endothelial cells that can account for up to 50% of all the CL cells [5]. On the other hand, steroidogenic large luteal cells and small luteal cells constitute about 30% of all CL cells [5,6]. The bovine CL also has various cell types such as smooth muscle cells, pericytes, fibrocytes and immune cells, indicating that the CL is a heterogeneous tissue [7,8]. In addition, the CL is constituted by the condensed blood vasculature including large blood vessels and microcapillaries, and the majority of steroidogenic cells are adjacent to one or more capillaries [9]. It is known that a cell-to-cell interaction and these adhesions are essentially important for the CL integrity and physiolog...

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Vascular composition, apoptosis, and expression of angiogenic factors in the corpus luteum during prostaglandin F2α-induced regression in sheep

Cited by 69 publications

References 62 publications

Cellular proliferation and vascularization in ovine fetal ovaries: effects of undernutrition and selenium in maternal diet

Cellular proliferation and vascularization in ovine fetal ovaries: effects of undernutrition and selenium in maternal diet

The corpora lutea proangiogenic state of VEGF system components is turned to antiangiogenic at the later phase of the oestrous cycle in cows

Nitric Oxide and Luteal Blood Flow in the Luteolytic Cascade in the Cow

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