Vascular Endothelial Growth Factor (VEGF) in Human Tooth Germ Center

Mastrangelo, Filiberto; Piccirilli, Marcello; Dolci, Marco; Tetè, Stefano; Speranza, Lorenza; Patruno, Antonia; Gizzi, F.; Felaco, Mario; Artese, Luciano; Lutiis, Maria Anna De

doi:10.1177/039463200501800319

Cited by 13 publications

(8 citation statements)

References 36 publications

(51 reference statements)

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“…Vascular endothelial growth factor is an important signalling protein involved in both vasculogenesis (the de novo formation of the embryonic circulatory system) and angiogenesis (the growth of blood vessels from preexisting vasculature). The presence of VEGF in enamel organs has previously been documented (29), and VEGF is closely associated with the differentiation of the inner enamel epithelium to ameloblasts (30). Monocyte chemotactic protein 1 and VEGF are expressed in the dental follicle and participate in tooth eruption by promoting osteoclastogenesis (31).…”

Section: Discussionmentioning

confidence: 97%

Serotonin and fluoxetine receptors are expressed in enamel organs and LS8 cells and modulate gene expression in LS8 cells

Riksen

Stunes

Kalvik

et al. 2010

European J Oral Sciences

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The selective serotonin re-uptake inhibitor (SSRI) fluoxetine is widely used in the treatment of depression in children and fertile women, but its effect on developing tissues has been sparsely investigated. The aim of this study was to investigate if enamel organs and ameloblast-derived cells express serotonin receptors that are affected by peripherally circulating serotonin or fluoxetine. Using RT-PCR and western blot analysis we found that enamel organs from 3-d-old mice and ameloblast-like cells (LS8 cells) express functional serotonin receptors, the rate-limiting enzyme in serotonin synthesis (Thp1), as well as the serotonin transporter (5HTT), indicating that enamel organs and ameloblasts are able to respond to serotonin and regulate serotonin availability. Fluoxetine and serotonin enhanced the alkaline phosphatase activity in the cell culture medium from cultured LS8 cells, whereas the expression of enamelin (Enam), amelogenin (Amel), and matrix metalloproteinase-20 (MMP-20) were all significantly down-regulated. The secretion of vascular endothelial growth factor (VEGF), monocyte chemotactic protein 1 (MCP-1), and interferon-inducible protein 10 (IP-10) was also reduced compared with controls. In conclusion, enamel organs and ameloblast-like cells express functional serotonin receptors. Reduced transcription of enamel proteins and secretion of vascular factors may indicate possible adverse effects of fluoxetine on amelogenesis.

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Section: Discussionmentioning

confidence: 97%

Serotonin and fluoxetine receptors are expressed in enamel organs and LS8 cells and modulate gene expression in LS8 cells

Riksen

Stunes

Kalvik

et al. 2010

European J Oral Sciences

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“…Therefore, during development, tooth morphogenesis progresses through mutually inductive signalling between interacting oral ephitelium and mesenchymal cells derived from the neural crest (20,(22)(23)(24) …”

Section: Mesenchymal Stem Cells and Teethmentioning

confidence: 99%

Adult Mesenchymal Stem Cells in Dental Research: A New Approach for Tissue Engineering

Trubiani¹,

Orsini²,

Caputi³

et al. 2006

Int J Immunopathol Pharmacol

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Many adult tissues contain a population of stem cells that have the ability to regenerate after trauma, disease or aging. Recently, there has been great interest in mesenchymal stem cells and their roles in maintaining the physiological structure of tissues. The studies on stem cells are thought to be very important and, in fact, it has been shown that this cell population can be expanded ex vivo to regenerate tissues not only of the mesenchymal lineage, such as intervertebral disc cartilage, bone and tooth-associated tissues, but also other types of tissues. Several studies have focused on the identification of odontogenic progenitors from oral tissues, and it has been shown that the mesenchymal stem cells obtained from periodontal ligament and dental pulp could have similar morphological and phenotypical features of the bone marrow mesenchymal cells. In fact a population of homogeneous human mesenchymal stem cells derived from periodontal ligament and dental pulp, and proliferating in culture with a well-spread morphology, can be recovered and characterized. Since these cells are considered as candidates for regenerative medicine, the knowledge of the cell differentiation mechanisms is imperative for the development of predictable techniques in implant dentistry, oral surgery and maxillo-facial reconstruction. Thus, future research efforts might be focused on the potential use of this cell population in tissue engineering. Further studies will be carried out to elucidate the molecular mechanisms involved in their maintenance and differentiation in vitro and in vivo.

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“…31,40 During treatment, decreasing PCT levels reflected defervescence, rapid or gradual clinical improvement and successful anti-microbial therapy, irrespective of the kind of bacterial infection. [49][50][51][52][53][54][55] The PCT concentration was higher in microbial infected than in non-microbial infected patients through the study period (P<0.001). The admission values of CRP were higher in the neutropenic leukemic patients with infection than in leukemic patients without infection .…”

Section: Discussionmentioning

confidence: 93%

Serial procalcitonin responses in infection of children with secondary immunodeficiency

Hatzistilianou

Rekleity

Athanassiadou

et al. 2007

CIM

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Purpose: Procalcitonin has proven to be a sensitive inflammatory marker in non-neutropenic patients. The aim of this study was to determine and compare Procalcitonin with other inflammatory markers in the serum of immunosuppressed children with haematological malignancies; and to assess the predictive value of these mediators in distinguishing between bacterial and non-bacterial infection. Methods & Results: The study included 37 children with acute lymphoblastic leukaemia undergoing intensive chemotherapy. They were divided into 3 groups, A, B and C. Group A consisted of 29 neutropenic children with 94 febrile episodes, group B of 20 neutropenic children with 56 afebrile episodes and group C of 13 non-neutropenic children with 58 afebrile episodes. Serial serum levels of PCT, C-Reactive Protein, Neopterin, Interleukin-6 and NO2/NO3 were all determined on a day-to-day basis for 7 consecutive days. In serum the concentrations of CRP was determined by nephelometry, of PCT by immunoluminescence and of Neopterin, IL-6 and NO2/NO3 by ELISA method. Conclusions: According to our results the Procalcitonin concentration increased rapidly in patients with microbial infection; the response was detectable within 24 hs of the onset of fever due to microbial infections. Procalcitonin is a spesific and sensitive marker of microbial infection in patients with neutropenic fever. The markers, C-Reactive Protein, Interleukin-6 and NO2/NO3 may not help to identify infections and distinguishe the etiology of infection in neutropenic febrile children with acute lymphoblastic leukaemia.

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Vascular Endothelial Growth Factor (VEGF) in Human Tooth Germ Center

Cited by 13 publications

References 36 publications

Serotonin and fluoxetine receptors are expressed in enamel organs and LS8 cells and modulate gene expression in LS8 cells

Serotonin and fluoxetine receptors are expressed in enamel organs and LS8 cells and modulate gene expression in LS8 cells

Adult Mesenchymal Stem Cells in Dental Research: A New Approach for Tissue Engineering

Serial procalcitonin responses in infection of children with secondary immunodeficiency

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