Vascular Grafts and the Endothelium

Hoenig, M.; Campbell, Gordon; Campbell, Julie H.

doi:10.1080/10623320601061615

Cited by 36 publications

(36 citation statements)

References 217 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…12). We have also confirmed (M. Hoenicka, unpublished observations) the finding of many studies, reviewed in 16 , that freshly seeded ECs do not deteriorate under similar conditions. As the bovine vessels in our study were without a proper oxygenation for at least 30 min, an ischemia/reperfusion damage is a reasonable explanation for the loss of ECs in native vessels.…”

Section: Discussionsupporting

confidence: 76%

See 1 more Smart Citation

Effects of Shear Forces and Pressure on Blood Vessel Function and Metabolism in a Perfusion Bioreactor

et al. 2010

View full text Add to dashboard Cite

Bovine saphenous veins (BSV) were incubated in a perfusion bioreactor to study vessel wall metabolism and wall structure under tissue engineering conditions. Group 1 vessels were perfused for 4 or 8 days. The viscosity of the medium was increased to that of blood in group 2.Group 3 vessels were additionally strained with luminal pressure. Groups 1-d through 3-d were similar except that BSV were endothelium-denuded before perfusion. Groups 1-a through 3-a used native vessels at elevated flow rates. Group 3 vessels responded significantly better to noradrenaline on day 4, whereas denuded vessels showed attenuated responses (p<0.001). Tetrazolium dye reduction did not depend on perfusion conditions or time except for denuded vessels. pO 2 gradients across the vessels were independent of time and significantly higher in group 2 (p<0.001). BSV converted glucose stoichiometrically to lactate except vessels of groups 3, 1-d, and 3-d which released more lactate than glucose could supply (p<0.001). Group 1 vessels as well as all vessels perfused with elevated flow rates showed a loss of endothelial cells after 4 days, whereas group 2 and 3 vessels retained most of the endothelium. These data suggest that vessel metabolism was not limited by oxygen supply. Shear forces did not affect glucose metabolism but increased oxygen consumption and endothelial cell survival. Luminal pressure caused the utilization of energy sources other than glucose, as long as the endothelium was intact. Therefore, vessel metabolism needs to be monitored during tissue engineering procedures which challenge the constructs with mechanical stimuli.

show abstract

Section: Discussionsupporting

confidence: 76%

“…Small caliber vascular grafts require a functional antithrombotic surface to avoid early graft failure. Dacron or poly(tetrafluoroethene) (PTFE), which are excellent materials to create large-caliber grafts, are therefore not well suited for coronary bypass surgery 16 or for the treatment of critical lower limb ischemia 20 .…”

Section: Introductionmentioning

confidence: 99%

Effects of Shear Forces and Pressure on Blood Vessel Function and Metabolism in a Perfusion Bioreactor

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Наша команда уже несколько лет изучает проблему взаимодействия организма с биосовместимыми биорезорбируемыми полимерами на клеточном и тканевом уровне [2,3,4]. Данная работа ведется с целью создания полимерных конструкций с био-направленным действием, способных заменить отдельные структуры живого организма, в част-ности, сердечно-сосудистой системы.…”

unclassified

“…Наиболее предпочтительным и физиологич-ным вариантом повышения биосовместимости поверхности сердечно-сосудистых имплантатов является ее эндотелизация, обеспечивающая за-щиту от тромбообразования и гиперплазии нео-интимы [2]. Эндотелизацию графтов возможно проводить in vitro, однако данный подход высо-котехнологичный и требует много времени, по-этому его использование будет ограничено пла-новыми вмешательствами [3].…”

unclassified

Modification by RGD-Peptides of Vascular Grafts of Small Diameter From Polyparolactone: Experimental Study Results

Матвеева¹,

Антонова²,

Sevost'yanova³

et al. 2017

Kompleks. probl. serdečno-sosud. zabol.

View full text Add to dashboard Cite

“…Synthetic polymers turned out to be unsuitable to build small caliber vessel grafts due to their limitations in terms of compliance and surface properties [1]. Instead, various approaches have been suggested to create vessel grafts from natural or biocompatible scaffolds seeded with vascular cells [2].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Requirements of Blood Vessels in a Perfusion Bioreactor

et al. 2009

View full text Add to dashboard Cite

Abstract-Small caliber vessel grafts are one of the major challenges of vascular tissue engineering. A variety of processes have been developed to create vascular grafts from scaffolds and donor cells in bioreactors. In order to optimize such processes, this study focused on monitoring vessel metabolism under conditions typically used in perfusion protocols. Bovine veins were perfused in a bioreactor for four days. Group 1 vessels served as controls and were perfused with standard medium. Medium of group 2 was adjusted to the viscosity of blood. Group 3 vessels were additionally challenged with elevated luminal pressure. Contractile function was assessed in an organ bath. Tissue viability was determined by tetrazolium dye reduction. Oxygen gradients, dextrose consumption, and lactate production were monitored using a blood gas analyzer. KCl induced contractions did not differ between days 0 and 4. Norepinephrine dose-response curves of group 3 vessels achieved a higher maximum contraction on day 4, with no changes of EC 50 . Tissue viability was not altered by any of the perfusion conditions. Oxygen gradients across the vessels did not change with time but were elevated in group 2, with no signs of oxygen depletion. Dextrose consumption and lactate formation of group 1 and 2 vessels appeared to be stoichiometric. In contrast, group 3 vessels produced more lactate than dextrose could supply. These results indicate that conventional oxygenation of culture media is sufficient to meet the oxygen consumption of a functional vessel. Elevated shear forces increased the oxygen demand without affecting dextrose consumption. Elevated shear forces and luminal pressure caused the utilization of alternative energy sources. Thus online monitoring of key metabolic parameters appears to be a desirable feature of perfusion bioreactors for vascular tissue engineering.

show abstract

Vascular Grafts and the Endothelium

Cited by 36 publications

References 217 publications

Effects of Shear Forces and Pressure on Blood Vessel Function and Metabolism in a Perfusion Bioreactor

Effects of Shear Forces and Pressure on Blood Vessel Function and Metabolism in a Perfusion Bioreactor

Modification by RGD-Peptides of Vascular Grafts of Small Diameter From Polyparolactone: Experimental Study Results

Metabolic Requirements of Blood Vessels in a Perfusion Bioreactor

Contact Info

Product

Resources

About