Vascularization and gene regulation of human endothelial cells growing on porous polyethersulfone (PES) hollow fiber membranes

Unger, Ronald E.; Peters, Kirsten; Huang, Qiang; Funk, Andreas; Paul, D.; Kirkpatrick, Charles James

doi:10.1016/j.biomaterials.2004.09.047

Cited by 81 publications

(52 citation statements)

References 26 publications

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“…Hence, in light of the critical role of ECs in the angiogenic process, a necessary step to evaluate and properly predict the vascularization potential of biomaterials is to assess the interaction of ECs with the respective substrate. [149][150][151] A great number of works have examined different aspects of ECs such as cell attachment, viability, growth, and phenotypic/genotypic expression on different bone substitutes: collagen, [152] silk fibroin, [153] polyethersulfone, [151] polycaprolactone (PCL), [154] and PLGA. [149] SPCL, a fiber-mesh scaffold and a material proposed by our group for bone regeneration, [13,94,[99][100][101] was also revealed to be compatible and a rather good substrate for ECs.…”

Section: Mature and Precursor Endothelial Cellsmentioning

confidence: 99%

Vascularization in Bone Tissue Engineering: Physiology, Current Strategies, Major Hurdles and Future Challenges

Santos

Reis

2009

Macromolecular Bioscience

389

273

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The lack of a functional vascular supply has, to a large extent, hampered the whole range of clinical applications of 'successful' laboratory-based bone tissue engineering strategies. To the present, grafts have been dependent on post-implant vascularization, which jeopardizes graft integration and often leads to its failure. For this reason, the development of strategies that could effectively induce the establishment of a microcirculation in the engineered constructs has become a major goal for the tissue engineering research community. This review addresses the role and importance of the development of a vascular network in bone tissue engineering and provides an overview of the most up to date research efforts to develop such a network.

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Section: Mature and Precursor Endothelial Cellsmentioning

confidence: 99%

Vascularization in Bone Tissue Engineering: Physiology, Current Strategies, Major Hurdles and Future Challenges

Santos

Reis

2009

Macromolecular Bioscience

389

273

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“…At each time period scaffolds (n = 3) were placed in fresh medium and 2-3μL calcein-AM (Mobitec, Germany) was applied. In viable (living) cells calcein-AM was converted into a fluorescent dye, resulting in the distribution of green fluorescence throughout the cell cytoplasm [35]. Specimens were incubated for 30mins at 37°C and analyzed using a confocal laser scanning microscopy (CLSM) (Leica TCS NT, Wetzlar, Germany).…”

Section: Cytotoxic Response After Cell Seedingmentioning

confidence: 99%

Comparative Characterisation of 3-D Hydroxyapatite Scaffolds Developed Via Replication of Synthetic Polymer Foams and Natural Marine Sponges

Cunningham¹,

Dunne²

2011

J Tissue Sci Eng

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The production of complex inorganic forms, based on naturally occurring scaffolds offers an exciting avenue for the construction of a new generation of ceramic-based bone substitute scaffolds. The following study reports an investigation into the architecture (porosity, pore size distribution, pore interconnectivity and permeability), mechanical properties and cytotoxic response of hydroxyapatite bone substitutes produced using synthetic polymer foam and natural marine sponge performs. Infiltration of polyurethane foam (60 pores/in2) using a high solid content (80wt %), low viscosity (0.126Pas) hydroxyapatite slurry yielded 84-91% porous replica scaffolds with pore sizes ranging from 50μm -1000μm (average pore size 577μm), 99.99% pore interconnectivity and a permeability value of 46.4 x10-10m2. Infiltration of the natural marine sponge, Spongia agaricina, yielded scaffolds with 56-61% porosity, with 40% of pores between 0-50μm, 60% of pores between 50-500μm (average pore size 349 μm), 99.9% pore interconnectivity and a permeability value of 16.8 x10-10m2. The average compressive strengths and compressive moduli of the natural polymer foam and marine sponge replicas were 2.46±1.43MPa/0.099±0.014GPa and 8.4±0.83MPa /0.16±0.016GPa respectively.Cytotoxic response proved encouraging for the HA Spongia agaricina scaffolds; after 7 days in culture medium the scaffolds exhibited endothelial cells (HUVEC and HDMEC) and osteoblast (MG63) attachment, proliferation on the scaffold surface and penetration into the pores.It is proposed that the use of Spongia agaricina as a precursor material allows for the reliable and repeatable production of ceramic-based 3-D tissue engineered scaffolds exhibiting the desired architectural and mechanical characteristics for use as a bone 3 scaffold material. Moreover, the Spongia agaricina scaffolds produced exhibit no adverse cytotoxic response.

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“…[2] Polysulfone (PSU) polymers are widely used in tissue engineering as biomaterials. [3] As biocompatible and non-degradable materials, these polymers have been used in a variety of bio-applications such as hemodialysis, [4] ultrafiltration, [5] filtration, [6] and bioreactor technology [7] as well as drug delivery [8] and cell culture applications. [9] In recent years, the main scientific and applied interest is focused not only on the synthesis of new types of polymeric materials but also on the modification of existing polymers to alter their properties to meet requirements for new applications.…”

Section: Introductionmentioning

confidence: 99%

Polysulfone/Pyrene Membranes: A New Microwell Assay Platform for Bioapplications

Karadag

Yılmaz

Toiserkani

et al. 2011

Macromolecular Bioscience

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The use of PSU-Py prepared by click chemistry as a platform in membrane-bottom microwell plates for oxidase and hydrolase/oxidase-based enzyme assays is studied. For the GOx assay, the postulated fluorescence mechanism is based on the consumption of glucose by dissolved oxygen and GOx in the microwell plates covered with the PSU-Py membrane. For the AG-GOx assay, maltose is used as AG substrate and hydrolyzed to glucose which is then oxidized by the GOx activity. It is shown that the PSU-Py membrane acts as a fluorescence indicator of the enzymatic reactions, and both GOx and AG/GOx enzyme assays are successfully applied for glucose, maltose and acorbose analysis in the range 0.125-2.0 × 10(-3) M glucose, 0.05-0.5 × 10(-3) M maltose, and 0.0125-0.1 mg · mL(-1) acorbose, respectively.

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Vascularization and gene regulation of human endothelial cells growing on porous polyethersulfone (PES) hollow fiber membranes

Cited by 81 publications

References 26 publications

Vascularization in Bone Tissue Engineering: Physiology, Current Strategies, Major Hurdles and Future Challenges

Vascularization in Bone Tissue Engineering: Physiology, Current Strategies, Major Hurdles and Future Challenges

Comparative Characterisation of 3-D Hydroxyapatite Scaffolds Developed Via Replication of Synthetic Polymer Foams and Natural Marine Sponges

Polysulfone/Pyrene Membranes: A New Microwell Assay Platform for Bioapplications

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